Juvenile exposure to vinclozolin shifts sex ratios and impairs reproductive capacity of zebrafish

Lor, Yer; Revak, Andrew; Weigand, Jenna; Hicks, Elisabeth; Howard, Donald; King‐Heiden, Tisha C.

doi:10.1016/j.reprotox.2015.09.003

Cited by 30 publications

(16 citation statements)

References 59 publications

(89 reference statements)

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“…The proportion of female H. azteca (as determined by the absence of male secondary sex characteristics) increased significantly following chronic FIN exposure to the highest concentration tested (96 mg/kg (2 mg/L)), whereas no effects on the proportion of females was observed following exposure to MGA (up to 76 mg/kg or 0.5 mg/L; Table 3 ). Altered sex ratios can arise upon exposure to estrogenic or antiandrogenic compounds from either sex-specific mortality (e.g., Versonnen et al 2004 ) or altered sexual differentiation (e.g., Lor et al 2015 ). It is unclear whether the observed change in sex ratio was due to sex-biased mortality, alteration of secondary sexual characteristics, or reduced growth/delayed development.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of the pharmaceuticals finasteride and melengestrol acetate to benthic invertebrates

Gilroy

Bartlett

Gillis

et al. 2020

Environ Sci Pollut Res

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The toxicity of endocrinologically active pharmaceuticals finasteride (FIN) and melengestrol acetate (MGA) was assessed in freshwater mussels, including acute (48 h) aqueous tests with glochidia from Lampsilis siliquoidea , sub-chronic (14 days) sediment tests with gravid female Lampsilis fasciola , and chronic (28 days) sediment tests with juvenile L. siliquoidea , and in chronic (42 days) sediment tests with the amphipod Hyalella azteca and the mayfly Hexagenia spp. Finasteride was not toxic in acute aqueous tests with L. siliquoidea glochidia (up to 23 mg/L), whereas significant toxicity to survival and burial ability was detected in chronic sediment tests with juvenile L. siliquoidea (chronic value (ChV, the geometric mean of LOEC and NOEC) = 58 mg/kg (1 mg/L)). Amphipods (survival, growth, reproduction, and sex ratio) and mayflies (growth) were similarly sensitive (ChV = 58 mg/kg (1 mg/L)). Melengestrol acetate was acutely toxic to L. siliquoidea glochidia at 4 mg/L in aqueous tests; in sediment tests, mayflies were the most sensitive species, with significant growth effects observed at 37 mg/kg (0.25 mg/L) (ChV = 21 mg/kg (0.1 mg/L)). Exposure to sublethal concentrations of FIN and MGA had no effect on the (luring and filtering) behaviour of gravid L. fasciola , or the viability of their brooding glochidia. Based on the limited number of measured environmental concentrations of both chemicals, and their projected concentrations, no direct effects are expected by these compounds individually on the invertebrates tested. However, organisms are exposed to contaminant mixtures in the aquatic environment, and thus, the effects of FIN and MGA as components of these mixtures require further investigation. Electronic supplementary material The online version of this article (10.1007/s11356-020-10121-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Toxicity of the pharmaceuticals finasteride and melengestrol acetate to benthic invertebrates

Gilroy

Bartlett

Gillis

et al. 2020

Environ Sci Pollut Res

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“…Other currentuse pesticides with endocrine disruptive properties are for example dicarboxamides like the viticultural fungicide vinclozolin (Kortekamp et al 2011). This fungicide has been shown to contribute to shifted sex ratios, an inhibited maturation and reduced fecundity as well as fertility in fish (Lor et al 2015). Although a few studies have explored endocrine disrupting effects of viticultural azole fungicides like tebuconazole and penconazole (e.g., Lv et al 2017;Poulsen et al 2015), they are not yet considered as EDCs by the Pesticide Properties DataBase (PPBD, Agriculture and Environment Research Unit of the University of Hertfordshire 2013) and the PAN International List of Highly Hazardous Pesticides (PAN List of HHPs; Pesticide Action Network International 2019).…”

Section: Introductionmentioning

confidence: 99%

Pesticide exposure affects reproductive capacity of common toads (Bufo bufo) in a viticultural landscape

2021

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Amphibian populations are declining worldwide at alarming rates. Among the large variety of contributing stressors, chemical pollutants like pesticides have been identified as a major factor for this decline. Besides direct effects on aquatic and terrestrial amphibian stages, sublethal effects like impairments in reproduction can affect a population. Therefore, we investigated the reproductive capacity of common toads (Bufo bufo) in the pesticide-intensive viticultural landscape of Palatinate in Southwest Germany along a pesticide gradient. In a semi-field study, we captured reproductively active common toad pairs of five breeding ponds with different pesticide contamination level and kept them in a net cage until spawning. Toads from more contaminated ponds showed an increased fecundity (more eggs) but decreased fertilization rates (fewer hatching tadpoles) as well as lower survival rates and reduced size in Gosner stage 25, suggesting that the higher exposed populations suffer from long-term reproductive impairments. In combination with acute toxicity effects, the detected sublethal effects, which are mostly not addressed in the ecological risk assessment of pesticides, pose a serious threat on amphibian populations in agricultural landscapes.

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“…Expected environmental concentrations for propiconazole have been suggested to be as high as 80 μg/L (DeLorenzo et al, 2001) but monitoring data again reports conazole fungicides at much lower concentrations (< 0.1 -7.7 μg/L) in aquatic environments (Haarstad, 2012;Wightwick et al, 2011). Both fungicides are considered anti-androgenic (Lor et al, 2015;Makynen et al, 2000;Skolness et al, 2013;van Ravenzwaay et al, 2013) and there is evidence that they also elicit oxidative stress (Bruno et al, 2009;Gazo et al, 2013;Radice et al, 1998;Tu et al, 2015). Due to substantial interest in endocrine disruption, studies assessing the toxic impacts of both fungicides have mainly focused on sexual development and related…”

Section: Introductionmentioning

confidence: 99%

“…reproductive performance, and primarily in rodents and fish (Bayley et al, 2002;Gazo et al, 2013;Hotchkiss et al, 2003;Lor et al, 2015;Makynen et al, 2000;Nesnow et al, 2011). However, as indicated this fails to consider possible subtle biochemical effects that might occur during early developmental life-stages, and overlooks a major class of globally threatened vertebrate species.…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiles of striped marsh frog (Limnodynastes peronii) larvae exposed to the anti-androgenic fungicides vinclozolin and propiconazole are consistent with altered steroidogenesis and oxidative stress

2018

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Amphibians use wetlands in urban and agricultural landscapes for breeding, growth and development. Fungicides and other pesticides used in these areas have therefore been identified as potential threats that could contribute towards amphibian population declines. However, relatively little is known about how such chemicals influence sensitive early life-stages or how short episodic exposures influence sub-lethal physiological and metabolic pathways. The present study applied untargeted metabolomics to evaluate effects in early post-hatch amphibian larvae exposed to the anti-androgenic fungicides vinclozolin and propiconazole. Recently hatched (Gosner developmental stage 25) striped marsh frog (Limnodynastes peronii) larvae were exposed for 96 h to vinclozolin at 17.5, 174.8 and 1748.6 nM and propiconazole at 5.8, 58.4 and 584.4 nM. Nuclear Magnetic Resonance (NMR) spectroscopy was performed on polar metabolites obtained from whole-body extracts. Both fungicides altered metabolite profiles compared to control animals at all concentrations tested, and there were notable differences between the two chemicals. Overall responses were consistent with altered steroidogenesis and/or cholesterol metabolism, with inconsistent responses between the two fungicides likely reflecting minor differences in the mechanisms of action of these chemicals. Broad down-regulation of the tricarboxylic acid (TCA) cycle was also observed and is indicative of oxidative stress. Interestingly, formic acid was significantly increased in larvae exposed to vinclozolin but not propiconazole, suggesting this metabolite may serve as a useful biomarker of exposure to androgen-receptor binding anti-androgenic contaminants. This study demonstrates the power of untargeted metabolomics for distinguishing between similarly acting, but distinct, pollutants and for unraveling non-endocrine responses resulting from exposure to known endocrine active contaminants.

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Juvenile exposure to vinclozolin shifts sex ratios and impairs reproductive capacity of zebrafish

Cited by 30 publications

References 59 publications

Toxicity of the pharmaceuticals finasteride and melengestrol acetate to benthic invertebrates

Toxicity of the pharmaceuticals finasteride and melengestrol acetate to benthic invertebrates

Pesticide exposure affects reproductive capacity of common toads (Bufo bufo) in a viticultural landscape

Metabolite profiles of striped marsh frog (Limnodynastes peronii) larvae exposed to the anti-androgenic fungicides vinclozolin and propiconazole are consistent with altered steroidogenesis and oxidative stress

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