K, Fy<sup>a</sup>, and Jk<sup>a</sup> phenotyping of donor RBCs on microplates

Diedrich, Beatrice; Andersson, Jan; Sallander, S.; Shanwell, Agneta

doi:10.1046/j.1537-2995.2001.41101263.x

Cited by 9 publications

(10 citation statements)

References 26 publications

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“…The Chinese tended to have a lower incidence of Le(a-b-) yet with a similar occurrence of Le(a+b+) at 12% [Table 6]. [4820]…”

Section: Discussionmentioning

confidence: 99%

“…[2] The ABO blood group and D status of blood donors and recipients are always taken into account when RBCs are transfused. [8] Other RBC antigens are usually not considered unless the recipient had previously undergone alloimmunization. This points out to the need to phenotyping RBC units to one or several antigenic systems, since only ABO and Rh (D) are usually typed.…”

Section: Introductionmentioning

confidence: 99%

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Red cell phenotyping of blood from donors at the National blood center of Malaysia

Musa

Ahmed

Hashim

et al. 2012

Asian J Transfus Sci

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Background:Human blood groups are polymorphic and inherited integral structures of the red cell membrane. More than 300 red cell antigens have been identified and further categorized into 30 major discrete systems. Their distribution varies in different communities and ethnic groups.Aims:This work was set to determine the prevalence of red cell phenotypes in donors from the major ethnic groups in Malaysia, namely, Malays, Chinese, and Indians.Materials and Methods:The work utilized the dextran acrylamide gel technique in which four types of gel cards were used to identify the blood groups of 594 subjects collected at the National Blood Transfusion Centre, Malaysia.Results:Blood group O and CDe/CDe (R1R1) were the most common in all ethnic groups. The cde/cde (rr) was more prevalent amongst Indians. The rare phenotypes found were cDE/cDE(R2R2) and cDE/CDE(R2Rz). With the Lewis system, the distribution of Le(a-b+) was similar among the ethnic groups. The rarest phenotype Fy(a-b-) was discovered in two donors. Jk(a-b-) was found in seven Malays and in two Indians. In the MNSs system, MN was common in Malays and Chinese, while the MM was more common among Indians. The rare SS was found in 19 donors. Malay and Chinese subjects had high P1 Negative blood but Indians showed high P1 positive blood. Within the Kell System, the very rare KK type was found in six subjects.Conclusions:The results obtained serve as an established database for the distribution of red cell phenotypes based on the blood group systems of donors from the major ethnic groups in Malaysia.

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“…The Chinese tended to have a lower incidence of Le(a-b-) yet with a similar occurrence of Le(a+b+) at 12% [Table 6]. [4820]…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Red cell phenotyping of blood from donors at the National blood center of Malaysia

Musa

Ahmed

Hashim

et al. 2012

Asian J Transfus Sci

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“…However, the phenotype of clinically significant blood group antigens on the donor red blood cells (RBCs) is required to be known at times when alloimmunization is particularly undesirable, such as in young females, pregnant women, and patients who are expected to require repeated transfusions in life, such as thalassemia or sickle cell disease patients. When selecting blood for transfusion to such patients, it would be useful if we have access to already phenotyped RBCs of donor population so that particular antigen typed blood can be given to such patients to prevent alloimmunization [5]. Furthermore, these are beneficial for already immunized patients if the transfusion is urgent and/or if clinically significant alloantibodies to particular antigen/antigens are present in the patient's serum.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, these are beneficial for already immunized patients if the transfusion is urgent and/or if clinically significant alloantibodies to particular antigen/antigens are present in the patient's serum. In such situations, corresponding antigen negative blood can be given to such recipients without much delay [5]. …”

Section: Introductionmentioning

confidence: 99%

Clinically Significant Minor Blood Group Antigens amongst North Indian Donor Population

Lamba

Kaur

Basu

2013

Advances in Hematology

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Background. Racial differences in blood group antigen distribution are common and may result in striking and interesting findings. These differences in blood group antigen distribution are important due to their influence on the clinical practice of transfusion medicine. Study Design and Methods. This is a prospective study, involving 1000 healthy regular repeat voluntary blood donors associated with the department. The clinically significant minor blood group antigens of these donors were studied. Results. Out of 1000 healthy regular repeat voluntary blood donors, 93% were D positive and 2.8% were K positive. Amongst the Rh antigens, e was the most common (99%), followed by D (93%), C (85.1%), c (62.3%), and E (21.5%). Within the MNS blood group system, antigen frequency was M (88%), N (57.5%), S (57.8%), and s (87.5%). Within the Duffy blood group system, antigen frequency was Fya (87.3%) and Fyb (58.3%). Conclusions. This data base will help us to prevent alloimmunisation in young females, pregnant women, and patients who are expected to require repeated transfusions in life by providing them with antigen matched blood. Antigen negative blood can also be made available without delay to already alloimmunized multitransfused patients.

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“…1–4 In the setting of stem cell therapy, delayed red cell engraftment complications due to alloimmunization can be obviated. 5–7 This prophylactic matching can be accomplished by serologic testing 8,9 or by red cell genotype ‘dry’ matching. 10 …”

Section: Introductionmentioning

confidence: 99%

Integration of red cell genotyping into the blood supply chain: a population-based study

Flegel

Gottschall

Denomme

2015

The Lancet Haematology

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Background When problems with compatibility arise, transfusion services often perform time-consuming serologic testing to locate antigen-negative red cell units for safe transfusion. New technologies enabled red cell genotyping for all clinically relevant blood group antigens. We performed mass-scale genotyping and provided access to a large red cell database to meet the demand for antigen-negative red cell units beyond ABO and Rh. Methods A red cell genotype database was established in 2010. Hospitals were given online access to a web-based antigen query portal in 2013 to find antigen-negative units in their inventories. Findings Genotype data were analyzed for 43,066 blood donors covering a set of 42 clinically relevant red cell antigens. Requests were filled for 5661 of 5672 patient encounters (99.8%) requiring antigen-negative red cell units in a multi-ethnic and multi-racial population. Red cell genotyping met the demand for antigen-negative blood in 5339 of 5672 (95%) patient encounters, while 333 remaining requests were filled using serologic data. In a pilot phase, seven community and rural transfusion services searched their local inventories using an online antigen query portal. Interpretation Red cell genotyping has the potential to transform the way antigen-negative red cell units are provided. An antigen query portal may reduce the need to ship blood or perform serologic screening. The wealth of genotype data, easily accessible online, facilitates the supply of affordable antigen-negative red cell units for patient safety. Physicians may recognize these new efficiencies for patient transfusion support. Funding BloodCenter of Wisconsin Diagnostic Laboratories Strategic Initiative and the NIH Clinical Center Intramural Research Program.

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K, Fy^a, and Jk^a phenotyping of donor RBCs on microplates

Abstract: The PEG-IAT microplate method gave reliable results that were suitable for routine phenotyping, thus making available a stock of phenotyped blood at reasonable cost, ready for delivery when required.

Cited by 9 publications

References 26 publications

Red cell phenotyping of blood from donors at the National blood center of Malaysia

Red cell phenotyping of blood from donors at the National blood center of Malaysia

Clinically Significant Minor Blood Group Antigens amongst North Indian Donor Population

Integration of red cell genotyping into the blood supply chain: a population-based study

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K, Fya, and Jka phenotyping of donor RBCs on microplates

Abstract: The PEG-IAT microplate method gave reliable results that were suitable for routine phenotyping, thus making available a stock of phenotyped blood at reasonable cost, ready for delivery when required.

Cited by 9 publications

References 26 publications

Red cell phenotyping of blood from donors at the National blood center of Malaysia

Red cell phenotyping of blood from donors at the National blood center of Malaysia

Clinically Significant Minor Blood Group Antigens amongst North Indian Donor Population

Integration of red cell genotyping into the blood supply chain: a population-based study

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Product

Resources

About

K, Fy^a, and Jk^a phenotyping of donor RBCs on microplates