The aim of this study was to determine the levels of Ni, Co, Pb, Cd and Hg in the muscle, hepatopankreas, kidney and gonads of European chub (Squalius cephalus) from the middle course of the Nitra River, during spring and autumn seasons by AAS method. The concentrations of metals (mg.kg-1 wet weight) in the muscle ranged as follows: Ni 0.15-1.18, Co 0.09-0.58, Pb 0.39-1.66, Cd 0.04-0.29, Hg 0.85-2.71. Statistically significant differences among individual tissues, as well as between seasons have been recorded. Higher metals concentrations were detected in inner organs than in muscle. Permissible limits for safe consumption in the case of Pb, Cd and Hg have been exceeded in 100%, 95% and 100%, respectively. Currently, for the Co and Ni are not set any permissible limits.