Kaempferol, the melanogenic component of Sanguisorba officinalis, enhances dendricity and melanosome maturation/transport in melanocytes

Tang, Huihao; Yang, Lili; Wu, Longlong; Wang, Huimin; Chen, Kaixian; Wu, Huali; Li, Yiming

doi:10.1016/j.jphs.2021.08.009

Cited by 25 publications

(21 citation statements)

References 33 publications

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“…Normal human foreskin-derived epidermal melanocytes were derived from young male adult foreskins (ethnic Han/aged 18–22 years). The detailed protocols were according to our team’s published research ( Tang et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Zebrafish embryos were cultured and selected from the Model Animal Research Center of Nanjing University (Nanjing, China); for the reproduction method, we referred to our team’s published research studies ( Tang et al, 2021 ). PTU was dissolved in water to make stock solutions and then diluted with fresh fish water to 200 mM for all treatments.…”

Section: Methodsmentioning

confidence: 99%

“…Normal human foreskin-derived epidermal melanocytes were derived from young male adult fore-skins (ethnic Han/aged 18–22 years). The detailed protocols were based on our previous publication ( Tang et al, 2021 ). Primary human melanocytes were treated with EFE (40 μg/ml) for 72 h. Total RNA was isolated for the construction of RNA-seq libraries.…”

Section: Methodsmentioning

confidence: 99%

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Epimedium brevicornum Maxim. Extract exhibits pigmentation by melanin biosynthesis and melanosome biogenesis/transfer

Chen

Yang²,

Zhang³

et al. 2022

Front. Pharmacol.

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Epimedium brevicornum Maxim. (Epimedii Folium) is a traditional medicine widely utilized in China for sexual dysfunction and osteoporosis treatment. Recently, studies have reported that Epimedium flavonoid icariin displayed hair growth and melanogenic ability by targeting tyrosinase activity. Nevertheless, icariin hydrolysate icariside II and icaritin cause depigmentation due to their tyrosinase inhibition. These pigment functional discrepancies from Epimedium constituents arouse our great interest. Then, this study focused on the pigmentation effects of Epimedii Folium extract (EFE) on melanin synthesis and melanosome biogenesis/transfer, and further identified the bioactive constituents. First, in in vitro systemic studies, we discovered that the potent melanogenic and repigmented effects of EFE were dependent on concentration and amount of time in multi-melanocytes, normal human skin tissue, and vitiligo perilesional areas. In vivo, EFE exhibited repigmented effect on two kinds of depigmented models of N-phenylthiourea-induced zebrafish and hydroquinone-induced mice. Mechanistically, EFE strongly promoted tyrosinase activity and upregulated the protein expression of tyrosinase families which finally contribute to melanin biosynthesis by activating the MAPK/ERK1/2 signal pathway. In addition, EFE effectively increased melanosome number, accelerated melanosome maturity and cytoplasmic transport through the growth/extension of melanocyte dendrites, and induced melanosome transfer from melanocyte to keratinocyte for pigmentation. The six main flavonoid ingredients were identified among EFE. Compared to others, epimedin B (EB) was confirmed as a high-content, low-toxicity, and effective melanogenic compound in EFE. Taking all these together, this study systematically demonstrates the potential pigmentation effect of Epimedium brevicornum Maxim., and clarifies its related molecular mechanisms and melanogenesis basis. These results give additional insight into Epimedium herb pharmacology and may provide a novel therapy basis for hypopigmentation disorders.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Epimedium brevicornum Maxim. Extract exhibits pigmentation by melanin biosynthesis and melanosome biogenesis/transfer

Chen

Yang²,

Zhang³

et al. 2022

Front. Pharmacol.

Self Cite

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“…Melanocyte biology is also controlled by fibroblasts, which secrete SCF and neuregulin 1 (NRG1), among others. Upon stimulation, melanocytes also secrete a number of signaling molecules such as pro-inflammatory cytokines including IL-1α, IL-2, IL-3, IL-6, IL-10 and tumor necrosis factor α (TNF-α), as well as chemokines including IL-8, CCL2 and transforming growth factor (TGF-β), α-MSH, catecholamines, eicosanoids, serotonin and nitric oxide (NO) [ 1 , 6 , 7 ].…”

Section: Melanocyte Biologymentioning

confidence: 99%

“…Further transformations involving TYR and TYRP1 finally produce the brown color of melanin (Figure 1). chemokines including IL-8, CCL2 and transforming growth factor (TGF-β), α-MSH, catecholamines, eicosanoids, serotonin and nitric oxide (NO) [1,6,7] Melanocytes synthesize melanin through a biochemical pathway called melanogenesis. The process takes place in separate cytoplasmic organelles called melanosomes.…”

Section: Melanocyte Biologymentioning

confidence: 99%

The Modulation of Melanogenesis in B16 Cells Upon Treatment with Plant Extracts and Isolated Plant Compounds

et al. 2022

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Plants are a rich source of secondary metabolites that exhibit numerous desired properties. The compounds may influence the biology of melanocytes, pigment cells that produce melanin, by modulating numerous signaling pathways, including cAMP/PKA, MAPKs and PI3K/AKT. Its downstream target is microphthalmia-associated transcription factor, responsible for the expression of the tyrosinase enzyme, which plays a major role in melanogenesis. Therefore, this literature review aims to provide insights related to melanogenesis modulation mechanisms of plant extracts and isolated plant compounds in B16 cells. Database searches were conducted using online-based library search instruments from 2012 to 2022, such as NCBI-PubMed and Google Scholar. Upregulation or downregulation of signaling pathways by phytochemicals can influence skin hypo- and hyperpigmentation by changing the level of melanin production, which may pose a significant cosmetic issue. Therefore, plant extracts or isolated plant compounds may be used in the therapy of pigmentation disorders.

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Germacrone, isolated from Curcuma wenyujin, inhibits melanin synthesis through the regulation of the MAPK signaling pathway

Li,

Chen,

Wang

et al. 2024

J Nat Med

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Kaempferol, the melanogenic component of Sanguisorba officinalis, enhances dendricity and melanosome maturation/transport in melanocytes

Cited by 25 publications

References 33 publications

Epimedium brevicornum Maxim. Extract exhibits pigmentation by melanin biosynthesis and melanosome biogenesis/transfer

Epimedium brevicornum Maxim. Extract exhibits pigmentation by melanin biosynthesis and melanosome biogenesis/transfer

The Modulation of Melanogenesis in B16 Cells Upon Treatment with Plant Extracts and Isolated Plant Compounds

Germacrone, isolated from Curcuma wenyujin, inhibits melanin synthesis through the regulation of the MAPK signaling pathway

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