Kainic acid increases the expression of the prohormone convertases furin and PC1 in the mouse hippocampus

Meyer, Arthur E.; Chretien, P; Massicotte, Guy; Sargent, Catherine; Chrétien, Michel; Marcinkiewicz, M.

doi:10.1016/0006-8993(96)00502-1

Cited by 32 publications

(25 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kainic acid-induced seizures have been reported to be associated with increased expression of the PCs furin and PC1 in the mouse hippocampus in a differential manner. 30 Many neuroprotective peptides may require posttranslational processing. They play a role in either protecting neurons from injury or in assisting them to recover from it.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Differentially Expressed Genes in Rat Hippocampus After Transient Global Cerebral Ischemia Using Subtractive cDNA Cloning Based on Polymerase Chain Reaction

Yokota

Uchijima

Nishizawa

et al. 2001

Stroke

View full text Add to dashboard Cite

Background and Purpose-The purpose of this study is to identify new molecules that play important roles in the phenomena that occur in the hippocampus after transient global cerebral ischemia, as clues to better understanding of the mechanisms. Methods-A subtractive cDNA library was established by suppression subtractive hybridization of rat hippocampal tissues after transient global cerebral ischemia. With differential screening of the library, upregulated fragments were identified. The mRNA expression levels of selected genes were measured with semiquantitative reverse transcriptase polymerase chain reaction (PCR). Results-Among more than 100 isolated fragments, approximately half were determined to be identical to known sequences. The rest showed high homology to known sequences, and only 2 did not exhibit homology to any known sequences. The expression of 5 genes identified in this study increased in 24 hours after ischemia to a level twice as high as that in sham-operated controls. These included furin, prosaposin, synaptotagmin IV, heat shock protein 105, and the neutral and basic amino acid transporter (NBAT). The increases in the mRNA expression levels of the genes except NBAT, as revealed by semiquantitative reverse transcription PCR, were statistically significant at both 6 and 24 hours after ischemia. Conclusions-Genes isolated are thought to be associated with production of proteins necessary for degeneration, neuroprotection, and reconstruction of neurons. How the expression of these genes relates to functional changes after ischemia remains to be determined. PCR-based subtractive cDNA cloning is demonstrated to be a useful tool for analyzing in vivo gene expression in animal ischemia models. (Stroke. 2001;32:168-174.)

show abstract

Section: Discussionmentioning

confidence: 99%

“…13 mRNA was isolated with oligo(dT) 30 -latex [Oligotex-(dT) 30 , Takara], and double-stranded cDNA (dscDNA) was synthesized by the method of Gubler and Hoffman 14 with oligo(dT) 18 primers, with the use of a cDNA synthesis kit (Takara).…”

Section: Rna Preparation and Double-stranded Cdna Synthesismentioning

confidence: 99%

Identification of Differentially Expressed Genes in Rat Hippocampus After Transient Global Cerebral Ischemia Using Subtractive cDNA Cloning Based on Polymerase Chain Reaction

Yokota

Uchijima

Nishizawa

et al. 2001

Stroke

View full text Add to dashboard Cite

show abstract

“…However, only a few studies have addressed the possible colocalization of PCs with neurotrophic factors in vivo. So far, furin and NGF have been colocalized by double in situ hybridization in the submaxillary gland (Meyer et al, 1996;Farhadi et al, 1997), whereas BDNF and PC1 were colocalized in the trigeminal ganglion (Meyer et al, 1996). Whether or not NGF and PCs colocalize in the injured sciatic nerve has not yet been demonstrated.…”

mentioning

confidence: 95%

Nerve growth factor and proprotein convertases furin and PC7 in transected sciatic nerves and in nerve segments cultured in conditioned media: Their presence in Schwann cells, macrophages, and smooth muscle cells

Marcinkiewicz

Chen

et al. 1999

J. Comp. Neurol.

View full text Add to dashboard Cite

Synthesis of proteins such as nerve growth factor (NGF) is induced after nerve lesion. The NGF precursor (pro‐NGF) requires a posttranslational processing by proprotein convertases to become active. In this report, we re‐examine the localization of NGF protein and mRNA in injured nerve and show that the candidate pro‐NGF convertases furin and PC7 colocalize with NGF in non‐neuronal cells in nerve. By Northern blot analysis, 1.5‐kb and 1.3‐kb NGF mRNAs were shown to be increased in distal and immediately proximal nerve segments on days 1, 4, and 14 after lesion; by Western blot analysis, NGF proteins of high molecular weight were detected after injury. In vivo, two phases of NGF immunopositivity were observed, in macrophages and perivascular cells shortly after lesion and in endoneurial cells on day 1 and 4. To identify the cells containing NGF, nerve segments were incubated in serum‐containing medium with or without conditioning by white blood cells isolated from the circulation. Both hybridization and immunoreactivity signals for NGF were elevated after incubation of nerve segments for 4 hours in conditioned media, so that cells with NGF immunoreactivity could be identified by antibodies to specific cell markers. In these nerve fragments, Schwann cells, perivascular smooth muscle cells, and macrophages contained NGF immunoreactivity. The concentration of furin and PC7 mRNA also increased in lesioned nerves. By immunocytochemical investigation of nerve explants, furin and PC7 were detected in endoneurial cells, macrophages and perivascular cells and were colocalized with NGF. These in vitro and in vivo findings suggest that both furin and PC7 are associated with NGF in several cell types of the sciatic nerve and, hence, may be implicated in intracellular processing of pro‐NGF. J. Comp. Neurol. 403:471–485, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

“…Plasmids for the prohormone convertases furin, PC1, and PC2 were prepared and used as described by Meyer et al (1996). NGF cDNA (Scott et al 1983) was inserted into the pGEM3Z plasmid, linearized, and used for synthesizing sense and anti-sense cRNA probes with SP6 and T7 polymerase.…”

Section: Complementary Rna Probesmentioning

confidence: 99%

Prohormone Convertases in Mouse Submandibular Gland: Co-localization of Furin and Nerve Growth Factor

Farhadi

Pareek

Day

et al. 1997

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

SUMMARY Nerve growth factor (NGF) in mouse submandibular glands (SGs) is generated from a 35-kD precursor by proteolytic enzymes that have yet to be identified. Prohormone convertases (PCs) cleave the NGF precursor in vitro, and in this study we questioned whether PCs could process salivary NGF in vivo. mRNA coding for PC2 (but not PC1) was detected on Northern blots of SG mRNA and also by in situ hybridization within parasympathetic neurons of intralobular ganglia. Northern blot and in situ hybridization analyses also detect mRNA coding for furin. In SGs of male mice, furin mRNA levels are high at birth and remain high throughout development. In glands from female mice, levels decline during postnatal development and are lower in adults than in newborns. Immunocytochemistry detects furin immunoreactivity in pro-acinar and ductal cells of glands from newborn and pubescent mice. In glands of adults, furin immunoreactivity is detectable in acinar cells but highest levels are present in NGF-containing granular convoluted tubule cells. These data, taken together with those from previous studies, suggest that furin is a candidate processing enzyme for NGF in mouse submandibular glands. (J Histochem Cytochem 45:795-804, 1997)

show abstract

Kainic acid increases the expression of the prohormone convertases furin and PC1 in the mouse hippocampus

Cited by 32 publications

References 53 publications

Identification of Differentially Expressed Genes in Rat Hippocampus After Transient Global Cerebral Ischemia Using Subtractive cDNA Cloning Based on Polymerase Chain Reaction

Identification of Differentially Expressed Genes in Rat Hippocampus After Transient Global Cerebral Ischemia Using Subtractive cDNA Cloning Based on Polymerase Chain Reaction

Nerve growth factor and proprotein convertases furin and PC7 in transected sciatic nerves and in nerve segments cultured in conditioned media: Their presence in Schwann cells, macrophages, and smooth muscle cells

Prohormone Convertases in Mouse Submandibular Gland: Co-localization of Furin and Nerve Growth Factor

Contact Info

Product

Resources

About