This study aims to develop an e-LKPD Based on the Nyeruit Ethnoscience, to describe its validity, practicality, and effectiveness to train Science Literacy on additives and addictive substances. Research and Development (R&D) is used as a research method based on Thiagarajan's four-dimensional model (Define, Design, Develop, Disseminate). 30 students participated as research subjects. Questionnaires were used to obtain validation data, learning practices, student response tests, and teacher responses. Efficacy is measured by pretests and posttests. The data analysis method used SPSS 24.0. As a result of this research and development, the development of an e-LKPD based on Nyeruit Ethnoscience has proven the effectiveness of the materials and media with a very high category of 84.7%. The utility of e-LKPD reaches 91.1% which is a very high standard. The effectiveness of the e-LKPD in teaching science literacy to students in the experimental class achieved a mean N-gain of 0.72, which was highly rated compared to the control class. The average N-gain is 0.54, indicating a moderate rating. Therefore, the development of an ethnoscience-based e-LKPD provides a large category effect size for improving students' science literacy.