Snack is food that is purchased in a ready-to-consume form and is widely preferred because of its relatively cheap price, easy to obtain, attractive and varied appearance. Rhodamine B dye is one of the colouring substances prohibited for food and is declared as hazardous substance according to Regulation of Health Minister Indonesia No. 722/Menkes/Per/IX/1988 regarding dyes that are declared dangerous and prohibited in Indonesia as well as in the Decree of the Directorate General of POM No. 239/Menkes/Per/V/1985 regarding certain dye stuffs declared as hazardous materials. This study aimed to recognize the levels of Rhodamine B dye in snacks traded. This was descriptive survey research with laboratory examination by analysing Rhodamine B dye on snacks by the results of UV-Vis spectrophotometric method. Population in this study were all snacks marketed in Karuwisi Traditional Market, while the sample was nine snacks consisting of: chili sauce, layer cake, red chocolate cream, red curly crackers, cassava chips, red jelly, red popcorn, cassava crackers, and macaroni crackers because the nine samples were physically identical to Rhodamine B colouring agent, namely the use of a striking red or pink colour. The sampling technique was total sampling. The results revealed that of the nine samples, only one sample was positive for Rhodamine B, namely cassava cracker containing 7.960μg/g. It concluded that red cassava cracker was a wholesale snack at Karuwisi Traditional Market which is positive for Rhodamine B.