Antibiotic resistance (ABR) is a global health problem. It is found not only in the health sector, but the ABR issue has to be tackled cross-sectors because the root cause of ABR is multi-sectors such as agriculture, livestock, environment sectors, and community. This study aims to assess the community as patients and farmers who use antibiotics with the knowledge, attitude, and practice (KAP) to ABR issue as scientifical information for improving health policy for the related sectors. The study was a cross-sectional survey of farmers and primary health care (PHC) patients in the Makassar sub-district of East Jakarta. Sample locations selected purposively, such as PHC and farms, have to be in the same subdistrict, and PHC has a minimum of 100 patients visit per day. The Slovin formula calculated the sample size for patients with a standard error of 5% and a simple random sampling method. Sampling calculated for farmers was total sampling, including farmers' assistants. There were 172 persons for patients and 16 persons for farmer respondents. Researchers asked respondents to fill in a questionnaire about the use of antibiotics properly, the efficacy of antibiotics, the potential harm of antibiotic & their practice of using antibiotics. The study started from August to December 2020. The data were analyzed descriptively. The results showed that the majority of respondents (>60%) were 26 to 45 years old, and most of them had senior high school education (>50%). In general, the KAP of most respondents was not good by means that only <50% of them gave proper answers to questions. Most respondents said antibiotics are most effective for relieving pain and healing influenza (±40%). The most answer to the potential harm of antibiotics is allergies and other adverse effects (30 to 45%). Only ±31% of respondents knew that irrational use of antibiotics leads to their resistance. Based on the study results, the PHC should educate the community that lives in the PHC cover area including farmers, not only its patients. The PHC should also actively involve cross-sectors such as the agriculture and livestock sector, the organization of family and community empowerment and welfare, and other related sectors to educate its community to understand better and be aware of the potential harm of ABR. Without multi-sector collaboration or a One Health approach, ABR control is complicated to do.