Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells

Tuerxuntayi, Adila; Liu, Yongqiang; Tulake, Ablajan; Kabas, Maidina; Eblimit, Aiden; Aisa, Haji Akber

doi:10.1186/1472-6882-14-166

Cited by 79 publications

(61 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kaliziri is a plant that grows only in the south of Xinjiang in China, and the highlands of Pakistan and India. The extract of this plant has been found to increase tyrosinase, MITF, TRP‐1, and TRP‐2 expression [Tuerxuntayi et al, ]. Furthermore, Matsuyama et al [] studied Capparis spinose plants that were used for spices, diuretics, and hypertension treatment in ancient times and found that the plant extract could increase the melanin content and tyrosinase mRNA of B16 melanoma cells.…”

Section: Discussionmentioning

confidence: 99%

Synergistic effect of rice bran extract and extremely low‐frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis

Kwon

Kim

Jang

et al. 2018

Bioelectromagnetics

View full text Add to dashboard Cite

Melanocytes in hair are located around dermal papilla cells at the tip of the hair follicle. In this study, we examined the melanogenesis of a three‐dimensional (3D) hair dermal papilla model treated with natural extracts and electromagnetic fields (EMFs). The 3D model involved dermal papilla‐like tissue (DPLT), an aggregation of a mixture of dermal papilla cells, and melanocytes in microwells. Rice bran extract (RBE), an EMF, and RBE/EMF were applied to different DPLT groups. The LDH assay indicated no cell stress in all experimental groups, and detection of tyrosinase activity demonstrated high activity in the RBE/EMF group. Western blot analysis of the RBE, EMF, and RBE/EMF groups revealed increased MITF, TRP‐1, and tyrosinase expression. In addition, the mRNA expression of ET‐1, laminin, bFGF, β‐catenin, MITF, and tyrosinase was increased in the RBE/EMF group, as demonstrated by RT‐qPCR analysis. HMB45 and Fontana–Masson immunostaining showed that the RBE/EMF group had the highest melanin content. Therefore, RBE and EMF may be used as a material and therapy, respectively, for the treatment of vitiligo and white hair, through activation of melanogenesis in melanocytes. Bioelectromagnetics. 39:595–603, 2018. © 2018 The Authors. Bioelectromagnetics Published by Wiley Periodicals, Inc..

show abstract

Section: Discussionmentioning

confidence: 99%

Synergistic effect of rice bran extract and extremely low‐frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis

Kwon

Kim

Jang

et al. 2018

Bioelectromagnetics

View full text Add to dashboard Cite

show abstract

“…Butin is one of the main flavonoids in the seeds of V. anthelmintica, which for centuries has been used in Uyghur medicine for the treatment of vitiligo. 'Kaliziri' extract, from seeds of loss of V. anthelmintica, increased the expression of TYR, TRP-1, and TRP-2 via Microphthalmia transcription factor (MITF) in B16 cells (Tuerxuntayi et al, 2014); thus, we have investigated the effects of butin in the vitiligo mice model and its possible mechanisms of action.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Butin on the Vitiligo Mouse Model Induced by Hydroquinone

Huo

Wang

Liu

et al. 2017

Phytotherapy Research

View full text Add to dashboard Cite

Vernonia anthelmintica (L.) Willd has been traditionally used in the treatment of vitiligo in Uyghur medicine. This study used butin, the main component of V. anthelmintica, to study the influence on hydroquinone-induced vitiligo in mice. The animals were randomly divided into six groups: control, model, 8-methoxypsoralen (8-MOP, 4.25 mg/kg), and butin (0.425, 4.25, and 42.5 mg/kg) groups. The number of melanin-containing hair follicles, basal layer melanocytes, melanin-containing epidermal cells, the expression of tyrosinase (TYR) and tyrosinase-related protein-1 (TRP-1), the malondialdehyde (MDA), and cholinesterase (CHE) activity in serum were measured. Our results indicated that compared with the model group, the melanin-containing hair follicles, the expression of TYR and TRP-1 increased, the activity of CHE decreased after treatment with 8-MOP and all doses of butin (p < 0.05, p < 0.01), the basal layer melanocytes and melanin-containing epidermal cells increased significantly after treatment with butin 4.25 and 42.5 mg/kg (p < 0.05, p < 0.01), and the MDA activity decreased after using butin 4.25 and 42.5 mg/kg and 8-MOP (p < 0.05, p < 0.01). Our results support the use of butin on vitiligo, and its possible mechanisms may be related to increase the TYR and TRP-1 protein expression and decrease the activity of MDA and CHE in hydroquinone-induced vitiligo model in mice. Copyright © 2017 John Wiley & Sons, Ltd.

show abstract

“…e HEMs were treated with NaHS at the indicated concentration for 24 h and then incubated in normal medium for another 24 mentation diseases such as albinism and vitiligo. Several previous studies have shown some substances have a regulatory effect on melanin synthesis [21][22][23]. Compared with these agents, the therapeutic advantage of H 2 S is that it is a kind of small molecular weight, water-and lipid-soluble intrinsic gasotransmitter that can freely penetrate into the cell membrane to exert a biological effect.…”

Section: Discussion/conclusionmentioning

confidence: 99%

Hydrogen Sulfide Promotes Cell Proliferation and Melanin Synthesis in Primary Human Epidermal Melanocytes

Ying

Wang

Jiang

et al. 2020

Skin Pharmacol Physiol

View full text Add to dashboard Cite

Background/Aim: Hydrogen sulfide (H 2 S) has been found to act as a physiological intercellular messenger to regulate cell survival. In this study, we evaluated whether H 2 S could promote cell proliferation and melanin synthesis in human epidermal melanocytes (HEMs). Methods: Primary HEMs were cocultured with sodium hydrosulfide (NaHS, the most widely used H 2 S donor) or endogenously overexpressed with cystathionine-γ-lyase (CSE) gene, which is the most predominant H 2 S-producing enzyme. Then, cell viability, intracellular melanin content, tyrosinase (TYR) activity, and expression of microphthalmia-associated transcription factor (MITF), TYR, together with TYR-related protein 1 (TRP-1) in both transcript and protein levels, were detected. Results: We first confirmed that NaHS (10-100 μm) increased cell viability, intracellular melanin content, and TYR activity in a dose-dependent manner. Then, we found that endogenous H 2 S production also promoted cell proliferation, intracellular melanin content, and TYR activity. In addition, we observed the mRNA and protein expression of MITF, TYR, and TRP-1 was significantly up-regulated after NaHS treatment and CSE gene transfection. Conclusions: This study demonstrates that H 2 S promotes cell proliferation and melanin synthesis in HEMs, which indicates pharmacologic regulation of H 2 S may be potential treatment for skin disorders caused by loss of melanocytes or dysfunction of melanogenesis.

show abstract

Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells

Cited by 79 publications

References 28 publications

Synergistic effect of rice bran extract and extremely low‐frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis

Synergistic effect of rice bran extract and extremely low‐frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis

The Effect of Butin on the Vitiligo Mouse Model Induced by Hydroquinone

Hydrogen Sulfide Promotes Cell Proliferation and Melanin Synthesis in Primary Human Epidermal Melanocytes

Contact Info

Product

Resources

About