Kallikrein-Like Activity in Nonpregnant and Pregnant Rat Uterus, Fetal Membranes, Placenta and Amniotic Fluid

Rm, Miatello; Mc, Lama; Es, González; Hl, Nolly

doi:10.1007/978-3-0348-7321-5_14

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…The need to incubate with trypsin extracts that has undergone detergent treatment and extensive dialysis to express the kininogenase activity suggests a latent form of the enzyme. This finding agrees with the need for prolonged autoincubation at 37°C and for acid treatment or trypsinization to reveal the enzymatic activity [8,9,42].…”

Section: Discussionsupporting

confidence: 89%

“…The presence of tissue kallikrein has been suggested by previous observations of a kininogenase activity in rat uterus [42] and in myometrium, placenta, amnion, and amniotic fluid of pregnant women and experimental animals [8,9]. The need to incubate with trypsin extracts that has undergone detergent treatment and extensive dialysis to express the kininogenase activity suggests a latent form of the enzyme.…”

Section: Discussionmentioning

confidence: 85%

“…'This work was supported by Grant 749/92 from Fondo Nacional de Ciencia y Tecnologia (Fondecyt) and a donation from Ricardo Claro. centa, amnion, and amniotic fluid [8,9]; and it has been postulated that kinins participate in the maintenance of the uteroplacental blood flow in established gestation [10]. This role is supported by the current belief that the kallikreinkinin system participates more in paracrine than in systemic control of blood flow [1,2].…”

Section: Introductionmentioning

confidence: 95%

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Uterine Kallikrein in the Early Pregnant Rat1

Valdés

Corthorn

Scicli

et al. 1993

Biology of Reproduction

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Uterine homogenates of cycling and early pregnant Sprague Dawley rats and purified rat urinary kallikrein showed similar curves of displacement of 125I-kallikrein binding to a polyclonal antibody. Uterine kallikrein concentration measured by RIA was 8.7 +/- 2 SEM ng/g wet weight during the cycle (n = 6 in diestrus and metestrus) and 20.8 +/- 2 SEM (n = 7) ng/g wet weight on Day 7 of pregnancy (P7) (p < 0.001). On P7, kallikrein concentration was increased 12.4-fold in the implantation nodes, as compared to the interimplantation segments. Uterine homogenates of rats on P7, submitted to DEAE-cellulose chromatography and Sephadex gel filtration, yielded two fractions containing kallikrein immunoreactivity and kininogenase activity, with molecular masses that ranged from 120-125 kDa and 39-43 kDa, respectively. In the RIA, both fractions displayed parallelism with purified kallikrein. Enzymatic activity was expressed after activation by trypsin. It was inhibited by aprotinin, PMSF, p-amino-benzamidine, and leupeptin, but not by soybean or ovomucoid trypsin inhibitors. Kallikrein mRNA was demonstrated by reverse transcriptase/polymerase chain reaction in uteri of nonpregnant and P7 rats. These results show that rat uterus synthesizes one or more serine proteases that are immunologically and enzymatically related to tissue kallikrein in the implantation node on P7--determined both by an increment of whole uterus kallikrein content and a depletion of the interimplantation segments--suggests that kallikrein may play a role in the vasoactive changes of implantation.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 95%

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Uterine Kallikrein in the Early Pregnant Rat1

Valdés

Corthorn

Scicli

et al. 1993

Biology of Reproduction

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Effects of Kinin Blockade on the Blood Pressure of Salt-Loaded Pregnant Rats

et al. 1995

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We evaluated whether chronic inhibition of bradykinin B2 receptors by the long-acting antagonist D-Arg, [Hyp3, Thi5,D-Tic7,Oic8]-bradykinin (Hoe 140) affects blood pressure of salt-loaded pregnant rats. Pairs of rats fed a high sodium diet (0.84 mmol sodium per gram chow) were mated at 14 weeks of age. Infusion of vehicle or Hoe 140 (300 nmol/d per kilogram body weight) was performed throughout each dam's pregnancy by use of an Alzet osmotic pump implanted in the abdominal cavity. In both groups, no significant change in systolic pressure (tail-cuff plethysmography) or renal blood flow (Doppler flow-meter) was observed from that in the unmated state to that at midterm pregnancy. In the control group, systolic pressure decreased at the 21st day of pregnancy (from 126 +/- 2 to 97 +/- 2 mm Hg, P < .01), and renal blood flow increased (from 6.1 +/- 0.1 to 7.5 +/- 0.2 kHz, P < .01). These changes were nullified by the administration of Hoe 140 (systolic pressure changing from 124 +/- 2 to 118 +/- 4 mm Hg, P = NS; renal blood flow changing from 6.3 +/- 0.2 to 6.2 +/- 0.1 kHz, P = NS). In the group given Hoe 140, placental weight was greater (0.50 +/- 0.01 versus 0.43 +/- 0.01 g in controls, P < .01) and the fetal/placental weight ratio was reduced (4.53 +/- 0.09 versus 5.31 +/- 0.17 in controls, P < .01).(ABSTRACT TRUNCATED AT 250 WORDS)

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Kallikrein-Like Activity in Nonpregnant and Pregnant Rat Uterus, Fetal Membranes, Placenta and Amniotic Fluid

Cited by 2 publications

References 6 publications

Uterine Kallikrein in the Early Pregnant Rat1

Uterine Kallikrein in the Early Pregnant Rat1

Effects of Kinin Blockade on the Blood Pressure of Salt-Loaded Pregnant Rats

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