Kallikrein-related peptidase 10 expression in salivary gland tissues                     and tumours

Darling, Mark; Hashem, Nelly N; Zhang, Irene; Mohamed, Aneez; Fung, Kevin; Kwan, Keith; Mara, Tom W.; Daley, Tom D.; Diamandis, Eleftherios P.

doi:10.5301/jbm.2012.10373

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…Human tissue kallikreins are a single family of 15 highly conserved trypsin and chymotrypsin-like serine proteases [54][55][56]. [50,53,57,58].…”

Section: General Overviewmentioning

confidence: 99%

“…They may display trypsin or chymotrypsin-like activity, or both [54,65]. KLKs share characteristic features, some of which overlap with all serine proteases, and those that are Many KLK genes exhibit copy number variations, which result from the use of alternative and multiple promotors, based on their 5'untranslated regions [60,67].…”

Section: Kallikreins As Proteases At the Genomic Mrna And Protein Lmentioning

confidence: 99%

“…An example is the coagulation cascade. Similarly, kallikreins are thought to participate in a variety of complex proteolytic cascade pathways, involved in the regulation of physiologic and pathologic processes [54]. These include: semen liquefaction, skin desquamation, neurodegeneration and tumour promoting or inhibiting effects [49].…”

Section: Kallikreins As Proteases At the Genomic Mrna And Protein Lmentioning

confidence: 99%

“…The highest expression of KLK8 is found in CNS, skin and ovarian tissues; it has also been found in esophagus, testis, tonsillar, renal, breast, and salivary gland tissue [54,71]. KLK8 has been demonstrated in a number of bodily fluids: breast milk, amniotic fluid, seminal fluid, and serum [54].…”

Section: Klk8mentioning

confidence: 99%

“…KLK8 has been demonstrated in a number of bodily fluids: breast milk, amniotic fluid, seminal fluid, and serum [54].…”

Section: Klk8mentioning

confidence: 99%

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Expression of Human Tissue Kallikreins (KLKs) in Polymorphous Low Grade Adenocarcinoma (PLGA)

Cox

2014

Journal of Oral and Maxillofacial Surgery

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Polymorphous low grade adenocarcinoma (PLGA) is the second most common malignant salivary gland tumour of the minor salivary glands. Human tissue kallikreins (KLKs) are a family of highly conserved serine proteases expressed by various tissues throughout the body. KLKs have become powerful tumour markers for the diagnosis of the cancer patient (e.g. PSA (KLK3)). The literature demonstrates a link between KLKs and salivary gland neoplasms. The purpose of this study is to determine levels of KLK mRNA in tissue samples of formalin fixed paraffin embedded polymorphous low grade adenocarcinoma (PLGA). Secondly, we wish to determine if KLK expression is limited to tumour cells alone.Nineteen cases of PLGA were reviewed . A diagnosis of PLGA was confirmed, demographic data was collected, and formalin fixed paraffin-embedded PLGA and normal salivary gland tissue samples were obtained. RNA isolation was achieved, followed by conversion to complementary DNA via reverse transcription. Synthesized DNA primers were added to target kallikrein DNA and through PCR, the quantitative level of expression of KLKs 1-15 was recorded. Samples exhibiting high and low KLK expression were selected for immunohistochemistry staining, using a standard protocol.Results from PCR data reveals statistically significant increase in the mean KLK mRNA expression for KLK1, KLK4, KLK10, KLK12 and KLK15 in PLGA tissue samples, as compared with normal salivary gland tissue (Mann Whitney U test, p<0.05).Immunohistochemistry results demonstrate tumour specific staining. Notably, all samples demonstrating relatively higher KLK mRNA expression showed equivalent or increased staining grade scores relative to the low KLK mRNA expression samples, with respect to a specific KLK.In tissue samples of polymorphous low grade adenocarcinoma there is an increase of KLK1, KLK4, KLK10, KLK12 and KLK15 mRNA relative to normal salivary gland tissue. Furthermore, the tumour cells stain positively and specifically for kallikreins.

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“…Human tissue kallikreins are a single family of 15 highly conserved trypsin and chymotrypsin-like serine proteases [54][55][56]. [50,53,57,58].…”

Section: General Overviewmentioning

confidence: 99%

Section: Kallikreins As Proteases At the Genomic Mrna And Protein Lmentioning

confidence: 99%

Section: Kallikreins As Proteases At the Genomic Mrna And Protein Lmentioning

confidence: 99%

Section: Klk8mentioning

confidence: 99%

“…KLK8 has been demonstrated in a number of bodily fluids: breast milk, amniotic fluid, seminal fluid, and serum [54].…”

Section: Klk8mentioning

confidence: 99%

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Expression of Human Tissue Kallikreins (KLKs) in Polymorphous Low Grade Adenocarcinoma (PLGA)

Cox

2014

Journal of Oral and Maxillofacial Surgery

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Functional Histology of Salivary Gland Pleomorphic Adenoma: An Appraisal

Triantafyllou

Thompson

Devaney

et al. 2014

Head and Neck Pathol

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The complex microstructure of salivary gland pleomorphic adenoma is examined in relation to function. Events related to secretion of macromolecules and absorption, responses to the altered microenvironment and controversies concerning epithelial-mesenchymal transition versus modified myoepithelial differentiation are explored. Their effects on tumor cell phenotypes and arrangements are emphasized. Heterotopic differentiation and attempts at organogenesis are also considered. The approach allows interpreting microstructure independently of histogenetic perceptions, envisaging the tumor cells as a continuum, endorsing luminal structures as the principal components, and defining pleomorphic adenoma as a benign epithelial tumour characterized by variable epithelial-mesenchymal transition, secretion/differentiation and metaplasia.

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Identifying Candidate Biomarkers for Pleomorphic Adenoma: A Case–Control Study

Morrison

Jackson-Boeters

Khan

et al. 2018

Head and Neck Pathol

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Pleomorphic adenoma (PA) is the most common benign salivary gland tumor. Kallikrein-related peptidases have been identified as biomarkers in many human tumors and may influence tumor behavior. We investigated KLK1-15 messenger ribonucleic acid and proteins in PA specimens to determine a KLK expression profile for this tumor. Fresh frozen PA tissue specimens (n = 26) and matched controls were subjected to quantitative real-time reverse transcription polymerase chain reaction to detect KLK1-15 mRNA. Expression of KLK1, KLK12, KLK13, and KLK8 proteins were then evaluated via immunostaining techniques. Statistical analyses were performed with the level of significance set at P < .05. We observed downregulation of KLK1, KLK12, and KLK13 mRNA expression, and immunostaining studies revealed downregulation of the corresponding proteins. Histologic evidence of capsular perforation was associated with increased KLK1 protein expression. Tumor size was not associated with capsular invasion and/or perforation. This study is the first to detail a KLK expression profile for PA at both the transcriptional level and the protein level. Future work is required to develop clinical applications of these findings.

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Kallikrein-related peptidase 10 expression in salivary gland tissues and tumours

Abstract: The finding of lower KLK10 levels in pleomorphic adenoma suggests aberrant expression in a tumour that develops primarily from myoepithelial cells. A kallikrein cascade may play a role in the development and/or outcome of some salivary gland tumours.

Cited by 7 publications

References 34 publications

Expression of Human Tissue Kallikreins (KLKs) in Polymorphous Low Grade Adenocarcinoma (PLGA)

Expression of Human Tissue Kallikreins (KLKs) in Polymorphous Low Grade Adenocarcinoma (PLGA)

Functional Histology of Salivary Gland Pleomorphic Adenoma: An Appraisal

Identifying Candidate Biomarkers for Pleomorphic Adenoma: A Case–Control Study

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