Kalman filtering-aided time-resolved solid-surface room temperature phosphorimetry for simultaneous determination of tetracyclines in solution

Alava-Moreno, Fausto; Liu, Yiming; Dı́az-Garcı́a, Marta Elena; Sanz‐Medel, Alfredo

doi:10.1007/bf01243320

Cited by 13 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, attractive features of implementing long-lived luminescent probes for DNA detection concern the large Stokes shift (or singlet−triplet splitting) that renders these probes very suitable for multiple labeling, the complete elimination of fast decaying background fluorescence, and the possibility of using time-resolved approaches for resolution of mixtures. In this connection, the difference between the RTP lifetime of the Pd−TMPyP bound to DNA and that bound to RNA could be exploited for simultaneous RTP determination of both nucleic acids, using a chemometric approach (e.g., Kalman filtering), as we have demonstrated in other RTP systems …”

Section: Resultsmentioning

confidence: 99%

Room-Temperature Phosphorescent Palladium−Porphine Probe for DNA Determination

1997

View full text Add to dashboard Cite

Room-temperature phosphorescence (RTP) enhancement of a palladium-porphine complex by DNA was studied. Studies involving calf thymus DNA and calf liver RNA revealed that the Pd-porphine complex bound to double-stranded DNA displayed intense RTP, while a very weak RTP emission was observed in the presence of equal molar concentrations of RNA. This fact founded a basis for selective determination of DNA in the presence of RNA. Maximum phosphorescence was observed at pH 7, with maximum excitation and emission wavelengths at 435 and 680 nm, respectively. Under optimal experimental conditions, the calibration graphs were linear up to 6 × 10(-)(5) μM DNA. The detection limit was 5 × 10(-)(8) μM and the relative standard deviation (10 replicates) ±2.6% in the linear range. DNA extracted from human colon tissues could be successfully determined.

show abstract

Section: Resultsmentioning

confidence: 99%

Room-Temperature Phosphorescent Palladium−Porphine Probe for DNA Determination

1997

View full text Add to dashboard Cite

show abstract

“…The solid-surface RTP was measured at the spectral maxima, l ex = 393 nm, l em = 615 nm. The Kalman filtering program was written in BASIC 19 and run on the Model 3600 data station. A link program between the spectrofluorimeter and the data interpreter was also written.…”

Section: Instrumentationmentioning

confidence: 99%

Kalman filtering-aided time-resolved solid-surface room temperature phosphorimetry for simultaneous determination of anthracyclines in solution

Alava-Moreno¹,

Valencia-González²,

Dı́az-Garcı́a³

1998

Analyst

Self Cite

View full text Add to dashboard Cite

Daunorubicin, doxorubicin and epirubicin react with EuIII to form complexes which exhibit analytically useful room temperature phosphorescence (RTP). The RTP features of the three complexes are similar and the RTP spectra completely overlap. However, their three phosphorescence decay rates are different, and these differences were utilized to analyse the time-resolved RTP data by Kalman filtering. Simultaneous quantification of all three complexes is demonstrated and a method is proposed for the simultaneous determination of the three anthracyclines in mixtures by RTP optosensing. The analytical errors observed are within +/- 5%.

show abstract

Phosphorescence Measurements, Applications of

Dı́az-Garcı́a

Badía

2000

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Luminescence is a term used for both fluorescence and phosphorescence. These are emission processes following the absorption of light, and the distinction between them is based upon the process of the deactivation mechanism. Phosphorescence has been identified as the light emitted from the triplet excited state to the ground state, a spin‐forbidden transition. Fluorescence is a radiational transition between electronic states of the same multiplicity. The first analytical uses of phosphorescence were published in the 1950s, but the technique was not widely accepted due to the practical aspect of measuring the signal at cryogenic temperatures (77 K). Developments in room temperature phosphorescence (RTP) have given rise to fundamental and practical advances which have stimulated interest in phosphorimetry. Solid‐surface room temperature phosphorescence (SSRTP) has been generally observed by immobilizing the lumiphor on a solid support such as filter paper or polymeric resins. SSRTP has been shown to be a sensitive and selective approach for the analysis of trace components in biological, pharmaceutical, and environmental samples. Techniques such as SSRTP combined with flow injection analysis provide new avenues for optical sensing transduction and improvement of SSRTP techniques. Applications of SSRTP in flowing systems include the sensing of cations, anions, oxygen, glucose, ethanol, antibiotics, and moisture. A number of developments in RTP have focused on sensitized phosphorescence (SP) and on the use of ordered media (micelles and cyclodextrins (CDs)), allowing the observation of RTP in fluid solution. In SP the triplet energy of a donor (analyte) is transferred to an acceptor that phosphoresces in fluid solution and the emission of the acceptor is measured. Therefore, SP can only provide quantitative information. In ordered media, a micelle (or a CD) protects the long‐lived triplet state from the quenching processes that normally take place in solution, allowing both quantitative and qualitative measurements. SP and ordered media RTP provide the limits of detection for many lumiphors in the nmol −1 range and calibration graphs have a wide linear range. As new instrumental and basic insights emerge in SSRTP, Micelle Stabilized Room Temperature Phosphorescence (MSRTP), and solution‐sensitized RTP, these techniques will find much greater use in the trace and ultratrace analysis of dissolved, volatile, and gaseous substances in areas such as pharmaceutical analysis, environmental science, and clinical chemistry.

show abstract

Kalman filtering-aided time-resolved solid-surface room temperature phosphorimetry for simultaneous determination of tetracyclines in solution

Cited by 13 publications

References 20 publications

Room-Temperature Phosphorescent Palladium−Porphine Probe for DNA Determination

Room-Temperature Phosphorescent Palladium−Porphine Probe for DNA Determination

Kalman filtering-aided time-resolved solid-surface room temperature phosphorimetry for simultaneous determination of anthracyclines in solution

Phosphorescence Measurements, Applications of

Contact Info

Product

Resources

About