Kaposi’s sarcoma in Morocco: a pathological study with immunostaining for human herpesvirus-8 LNA-1

Hbid, Oumkaltoum; Belloul, L.; Fajali, Noura; Ismaïli, Nadia; Duprez, Renan; Tanguy, Myriam; Benomar, Hakima; Tahri, El Hassane; Gessain, Antoine; Huerre, Michel

doi:10.1080/00313020500169453

“…All these formalin-fixed, paraffin-embedded biopsy samples were stained by using hematoxylin–eosin stain and Perl methods. Immunohistochemistry was performed on deparaffinized sections by using monoclonal antibodies directed against CD34 and latent nuclear antigen (LANA-1) ( 12 ). …”

Section: The Studymentioning

confidence: 99%

Human Herpesvirus 8 Genotype E in Patients with Kaposi Sarcoma, Peru

Cassar¹,

Blondot²,

Mohanna³

et al. 2010

Emerg. Infect. Dis.

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“…Elle est classée en 4 formes épidémiologiques: 1) MK classique, touchant le plus souvent les hommes âgés originaires de l'Europe centrale, l'Europe de l'Est et les méditerranéens avec une prédominance chez la population juive; 2) MK endémique (Afrique subsaharienne); 3) MK iatrogénique associée à une immunosuppression; 4) MK épidémique (associée au SIDA/ HIV) [3]. La MK classique se présente habituellement sous forme de macules violines et érythémateuses qui évoluent lentement vers des plaques et des nodules angiomateux.…”

Section: Discussionunclassified

Maladie de kaposi classique avec atteinte surrénalienne: un nouveau cas

Bouzidi¹,

Gallouj²,

Krich³

et al. 2014

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La maladie de kaposi est une pathologie connue généralement dans sa forme épidémique associée au sida ou celle endémique présente dans l'Afrique subsaharienne. La forme classique de cette maladie ou dite également méditerranéenne n'est pas bien connue étant assez rare. Elle touche les hommes âgés HIV séronégatifs originaires de l'Europe centrale, l'Europe de l'Est et les méditerranéens. Elle se manifeste essentiellement par une atteinte cutanée, éventuellement muqueuse mais l'atteinte viscérale reste moins fréquente, dominée par l'atteinte osseuse, pulmonaire et gastrique. La localisation surrénalienne est inhabituelle et très rare. Nous rapportons ce nouveau cas pour mettre le point sur cette pathologie rare et signaler cette localisation atypique.

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“…As Kaposi's sarcoma is infrequent in Pacific Islands, classical immunohistochemistry was performed on deparaffinised sections, using a panel of antibodies including anti-factor VIII, anti-CD31 and anti-CD34 antibodies as well as proliferation cell nuclear antigen antibodies (PCNA) and Ki67, as previously described (Hbid et al, 2005). The HHV-8-specific antibody directed against latent nuclear antigen (LNA-1 encoded by open reading frame 73), was provided by Novocastra (clone 13 B10) and used at a dilution of 1:30, as described elsewhere (Hbid et al, 2005).…”

Section: Pathology and Hhv-8 Serological Studiesmentioning

confidence: 99%

“…As Kaposi's sarcoma is infrequent in Pacific Islands, classical immunohistochemistry was performed on deparaffinised sections, using a panel of antibodies including anti-factor VIII, anti-CD31 and anti-CD34 antibodies as well as proliferation cell nuclear antigen antibodies (PCNA) and Ki67, as previously described (Hbid et al, 2005). The HHV-8-specific antibody directed against latent nuclear antigen (LNA-1 encoded by open reading frame 73), was provided by Novocastra (clone 13 B10) and used at a dilution of 1:30, as described elsewhere (Hbid et al, 2005). Two different immuno-fluorescence assays (IFA) were used, as previously described (Kedes et al, 1996;Plancoulaine et al, 2002): (i) a commercial test (HHV-8 immunofluorescence antibody assay; ABI, Columbia, MD), using the KS-1 cell line as a HHV-8 source of antigens and detecting antibodies mainly directed against lytic HHV-8 antigens and (ii) an in house test using BC-3 cells which express only HHV-8 latent antigens (LNA).…”

Section: Pathology and Hhv-8 Serological Studiesmentioning

confidence: 99%