Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)

Otaviano, A. R.; Tonhati, Humberto; Sena, Janete Apparecida Desidério; Muñoz, Mario

doi:10.1590/s1415-47572005000200010

Cited by 32 publications

(30 citation statements)

References 16 publications

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“…The A and B variants occur in amino acids located relatively close to several glycosylation sites such as amino acids in position 136 and 148 aa of primary structure. In this variation, threonine is replaced by isoleucine in position 136 aa, whereas aspartic acid is replaced by alanine in position 148 aa for A and B, respectively (Otaviano et al, 2005;Azevedo et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Analysis of selected milk traits in Palestinian Holstein-Friesian cattle in relation to genetic polymorphism

Khaizaran¹,

Al-Razem²

2014

J. Cell Anim. Biol.

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Modern dairy cattle breeding strategies depend on linkage analysis and quantitative trait loci (QTL) of genes involved in milk yield and composition. This is because of their biological desired quantitative traits that play key roles in milk production. In this study, three genes directly related to milk production: prolactin (PRL), bovine kappa-casein (K-CN) and the pituitary-specific transcription factor (PIT-1) were analyzed in 144 cows. The aim of this study was to identify polymorphisms in the HolsteinFriesian cattle breed in Palestine in relation to the genetic markers and allelic variants of the three genes. Collection of samples depended on an experimental design that was completely randomized (CRD) and blood samples were collected from different cities across the West Bank, Palestine. The genotypes were determined through the polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) technique. The amplified fragments of PRL (294-bp), K-CN (530-bp) and PIT-1 (451-bp) were digested with RsaI, HindIII and HinfI, respectively. Statistical analysis found that the prolactin allelic substitution (AG, GG) played a role in milk production with a p-value of 0.00643 and α (0.001**), the AG allele of PRL being more favorable for milk production as compared to the GG allele. Genetic variants of the bovine K-CN gene played a role in milk production with a p-value of 0.04071 and α (0.01*), the AA allele possessing more positive effect than the BB and AB alleles. Similarly, the allelic substitution of the PIT-1 gene affected milk production with a p-value of 2.274e-05 and α (0***), the AA allele exercising a more positive effect followed by the AB and BB alleles, respectively. Among the three studied breeds (Friesian, hybrid and local), results show that the Friesian breed possesses higher overall milk production in Palestine as compared to the other two breeds.

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Section: Introductionmentioning

confidence: 99%

Analysis of selected milk traits in Palestinian Holstein-Friesian cattle in relation to genetic polymorphism

Khaizaran¹,

Al-Razem²

2014

J. Cell Anim. Biol.

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“…However, traditional methods based on phenotype observations of cows and their progeny and kin are both time-consuming and expensive. With the advent of molecular techniques, greater productivity can be achieved by determining the potential of an animal even before phenotypes are expressed through the identification of genetic variants of any gene at the DNA level, independent of age and gender (Otaviano et al, 2005). Milk production traits in dairy cattle are governed mainly by genetic factors, whose variation can then be used as molecular markers for milk production traits (Ng-Kwai-Hang et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Association between a polymorphism of the α-lactalbumin gene and milk production traits in Chinese Holstein cows

Zhang¹,

Dong²

2013

Genet. Mol. Res.

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ABSTRACT. The traits particularly important for milk production include milk yield, protein percentage, fat percentage, and the somatic cell score. Alpha-lactalbumin (α-LA) is an important whey protein of cow milk, and is also present in the milk of many other mammalian species. In this study, we analyzed the genetic polymorphisms of the α-LA gene and their relationship to milk production traits (milk yield, protein percentage, fat percentage, and somatic cell score) in Chinese Holstein cows. The goal of this study was to contribute further molecular genetic information related to dairy cattle, to determine the molecular markers that are most closely linked with milk production traits, and to provide a scientific basis for the improvement of economically relevant traits in cows. Fluorescence-based conformation-sensitive gel electrophoresis, DNA sequencing, and ligation detection reaction techniques were used to analyze genetic variations of the α-LA gene (5ꞌ-UTR, exons 1, 2, 3, 4, and 3ꞌ-UTR) in 923 Chinese Holstein cows. One novel single nucleotide polymorphism (SNP), α-LA2516, was identified in exon 4 of the α-LA gene. Allele frequencies were as follows: T 0.674, C 0.326. Association analysis revealed that α-LA2516 was not associated with milk yield, These findings suggest that the SNP α-LA2516 in the α-LA gene likely does not have potential as a molecular marker for milk production traits in Chinese Holstein cows.

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“…The κ-Cn is considerably diff erent from other caseins in structure and other properties (Azevedo et al, 2008). The genetic polymorphism of the κ-Cn gene in ruminant has been well documented providing ca le (Pinders et al, 1991;Rĳ njkels et al, 1997), buff aloes (Pipalia et al, 2001;Otaviano et al, 2005;Patel et al, 2007;Riaz et al, 2008), goats (Angiolillo et al, 2002;Yahyaoui et al, 2003;Prinzenberg et al, 2005;Kiplagat et al, 2010), sheep (Moioli et al, 2007) and yak (Prinzenberg et al, 2008). Eleven variant alleles of the κ-Cn gene have been identifi ed in ca le, namely A, B, C, D, E, F, G, H, Al, and J (Soria et al, 2003).…”

Section: Kappa-casein Genotypic Frequencies Inmentioning

confidence: 99%

Kappa-Casein Genotypic Frequencies in Holstein-Friesian Dairy Cattle in West Java Province

Anggraini¹,

Sumantri²,

Farajallah³

et al. 2010

medpet

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Blood samples were also taken via cocsigalis vein of AI (active and non active) HF bulls at the two National AI Stations in Lembang (25 hds) and Singosari (32 hds). Identifi cation of genetic variationwas by applying PCR-RFLP method. Three genotypes were identifi ed, namely AA, AB, and BB, resulting two alleles of A and B. Results entirely showed that the frequencies of the BB HF female were very low, with the range of 0%-6% (vs AA genotype= 10%-54% and AB genotype= 46%-85%), despite of the relatively high frequency of the B allele over the A allele (23%-48% vs. 52%-77%). The low frequency of the observed BB females might be due to the limited AI active BB bulls used for services by the two national AI stations (0%-4%).

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Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)

Cited by 32 publications

References 16 publications

Analysis of selected milk traits in Palestinian Holstein-Friesian cattle in relation to genetic polymorphism

Analysis of selected milk traits in Palestinian Holstein-Friesian cattle in relation to genetic polymorphism

Association between a polymorphism of the α-lactalbumin gene and milk production traits in Chinese Holstein cows

Kappa-Casein Genotypic Frequencies in Holstein-Friesian Dairy Cattle in West Java Province

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