Karakterisasi dan Uji Sitotoksik Daun Jeruju (Acanthus illicifolius)

Suryati, Suryati; Husni, Elidahanum; Astuti, Winda; Nasty, Ranura

doi:10.25077/jsfk.5.3.207-211.2018

Cited by 6 publications

(8 citation statements)

References 6 publications

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“…The ethanol extract of yield of 19.6%, and n-hexane fraction of 4.3%. These results fraction yield a larger of previous research that is 2.2% 22 . The results of phytochemical screening on ethanol extracts contain flavonoid compounds with red due to colors, tannins (blue), terpenoids (green), alkaloids (orange to red), and steroids (yellow) (Tab 1, fig 1).…”

Section: Resultssupporting

confidence: 77%

The Antibacterial Activity of Acanthus ilicifolius L. n-Hexane Fraction

Warsinah

Wijaya

Ekowati

2022

JSTRP

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Background: Recently, with the high prevalence of diarrhoea caused by bacterial infection, the usage of antibiotics has increased. Antibiotic overuse might lead to several side effects and resistance, suggesting the development of an alternate antibacterial agent. A mangrove plant, Acanthus Illinois, contained triterpenoid, which has antibacterial properties. Aim: This study aimed to evaluate the antibacterial effect of the Acanthus Illinois n-hexane fraction against Escherichia coli and Shigella dysenteriae. Method: The Acanthus Illinois was fraction using n-Hexane, identification of secondary metabolite compound using GC-MS, and evaluation of antibacterial activity against Escherichia coli and Shigella dysenteriae under paper disc methods. This study was designed using a fully randomized design (CRD) with concentrations of fractions 1%, 2%, and 4%. The GC-MS results were compared to the WILEY 9 library and analysed. A prism graph was used to measure the inhibition zone of antibacterial activity. Result: The n-hexane fraction yield is 3.3% and contains sesquiterpene compounds (trans (beta.)-caryophyllene, alpha humulene, naphthalene decahedron-4A-methyl), terpenoid alcohol (3,7,11,15-tetramethyl -2-hexadecane-1-ol), and fatty acids (hexadecanoic acid methyl ester. Acanthus Illinois n-hexane fraction has antibacterial activity against Escherichia coli and Shigella dysenteriae in a dose-dependent manner. Conclusion: The n-hexane fraction of leaves Acanthus ilicifolius contains sesquiterpene, alcohol terpenoids, and fatty, and has antibacterial activity against Escherichia coli and Shigella dysenteriae

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Section: Resultssupporting

confidence: 77%

The Antibacterial Activity of Acanthus ilicifolius L. n-Hexane Fraction

Warsinah

Wijaya

Ekowati

2022

JSTRP

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“…In addition, jeruju leaf extract contains alkaloids compounds, flavonoids, polyphenols, phenyletanoid glycosides and coumarins. The methanol part of thistle leaf extract has a pharmacological effect to inhibit edema (inflammation) (Suryati et al, 2018).…”

Section: Plan Part (Leaf)mentioning

confidence: 99%

“…Mangroves have many benefits that are directly related to human life, ranging from ecological benefits to being a source of food and medicine. Many universities and research institutes have explored various plants as sources of medicine but have not looked at the benefits of mangroves (Suryati et al, 2018). Mangroves are a diverse group of salt-tolerant plants that grow in tropical and subtropical intertidal forests and represent a rich biodiversity of plants, animals and microorganisms (Cai et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Traditionally all parts of this plant are used as a treatment, as in the fruit part it is used for Aphrodisiac and asthma; on the part of the leaves and roots are used for antidiabetic, diuretic, hepatitis, leprosy; the bark of the steam is used for neuralgia, roundworms, rheumatism, skin disease, and abdominal pain. Decoction of jeruju leaves is mostly used for energy recovery after giving birth by boiling and used for bathing water, restoring stamina after giving birth and preventing infection in the uterus (Suryati et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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HPLC Determination of Benzoxazolinone on Jeruju Plant (Acanthus Ilicifolius): Narrative Review

Salamah

Hidayat

Kusuma

et al. 2023

JFSP

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Mangrove plants in Indonesia are the largest in the world, both in terms of area quantity (+42,550 km2) and number of species (+45 species). Mangroves are a diverse group of salt tolerant plants that grow in intertidal tropical and subtropical forests and represent a rich biodiversity of plants, animals and microorganisms. Acanthus ilicifolius is one of the true mangrove plants which is used as medicine. This literature review aims to determine the study of benzoxazolinone compounds in jeruju plants (Acanthus ilicifolius) from previous studies and to determine the methods, plant parts and solvents used. The literature review method related to the jeruju plant (Acanthus ilicifolius) uses an electronic data base google scholar. Nuclear Magnetic Resonance (NMR) spectroscopy analysis method is one of the spectroscopic methods widely used in previous studies, because NMR spectroscopy is useful in determining the structure of a chemical compound, both proton (1H) and carbon (13C) NMR. Jeruju leaf extract contains alkaloids, flavonoids, polyphenols, phenyletanoid glycosides and coumarin compounds. The methanol fraction of jeruju leaf extract has a pharmacological effect to inhibit edema (inflammation). The extraction method used is maceration, because this method is simple method and most widely used. Meanwhile, the solvents that are more widely used are methanol and ethanol solvents.

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“…Therefore, alternative treatment approaches to eliminate resistant tumor cells are needed (Zhang and Chen, 2018). Referring to the description of the explanation put forward by Suryati, et al (2018) explained that looking for sources of compounds found in plants can be used as another option in the process of curing cancer.…”

Section: Introductionmentioning

confidence: 99%

The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells

Maulana

2021

JSCRTE

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Disease cancer caused by abnormal growth of tissue where there has been an error, fast and out of control. Judging from the fact of gender, more than 270,000 women die every year caused by cervical cancer. To inhibit the growth of cancer cells, a compound is needed that causes the cell cycle to stop so that the ability of cell proliferation decreases. Alkaloid compounds can inhibit proliferation through oxidative inhibition processes that can cause cancer. Mangrove plants have potential as anticancer, antimicrobial, and antioxidant. The content of chemical compounds found in mangroves are flavonoids, steroids, alkaloids, phenolites, saponins and tannins. These compounds show high antioxidant activity and are shown to have a real relationship with the properties of the material's bioactivity against cancer cells. One of the mangrove species is Rhizophora apiculata. The purpose of this study was to determine the IC50 value produced by Rhizophora apiculata mangrove leaf extract on HeLa cell viability and to see the effect of Rhizophora apiculata mangrove leaf extract dosage on HeLa cell viability. The method used in this research is the experimental method. The research parameters included yield, proximate test, phytochemical test, toxicity test, total phenol test, cytotoxicity test and LC-MS test. The experimental design used was a simple and complex completely randomized design (CRD) with the Tukey test.The results of this study showed that the highest yield was in the ethanol extract of 5.91%, while the n-hexane and ethyl acetate extracts respectively had yields of 1.18% and 1.31%. The results of the proximate test on the water content of leaves and powder were 64.53% and 13.86%, respectively, the results of the ash content in the leaves and powder of Rhizophora apiculata were 3.94% and 8.41%, respectively. while the water content in the extract obtained the highest yield in the ethanol extract of 21.42%, while the n-hexane extract and ethyl acetate extract were 11.08% and 15.42%, respectively. For phytochemical results, it was found that n-hexane extract only contained alkaloids, flavonoids and steroids. Ethyl acetate extract contains steroid compounds. Meanwhile, the ethanol extract contains the most bioactive compounds, namely saponins, flavonoids, tannins and triterpenoids. The toxicity test using the Brine Shrimp Lethality Test (BSLT) method resulted in the lowest IC50 of ethanol extract at 49.45 ppm while the n-hexane and ethyl acetate extracts were 251.63 ppm and 920.45 ppm respectively. In the total phenol test, the n-hexane extract was 66.79 mg GAE / 100 gr, 222.97 mg GAE / 100 gr ethyl acetate extract and 929.04 mg GAE / 100 gr ethanol extract. HeLa cell cytotoxicity testing using the MTT method (3- (4,5-dimethiltiazol-2-yl) -2,5-dipheniltetra zolium bromide) assay resulted in the highest cell viability value at a dose of 125 ppm of 46.97%. As for the doses of 250 ppm, 500 ppm 1000 ppm, and 2000 ppm resulted in a percentage of viability of 42.95% 37.70% 35.82% and 32.12%, respectively. The IC50 value of Rhizophora apiculata leaf extract was 64.42 ppm. This value indicates that the Rhizophora apiculata extract is toxic to HeLa cells.

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Karakterisasi dan Uji Sitotoksik Daun Jeruju (Acanthus illicifolius)

Cited by 6 publications

References 6 publications

The Antibacterial Activity of Acanthus ilicifolius L. n-Hexane Fraction

The Antibacterial Activity of Acanthus ilicifolius L. n-Hexane Fraction

HPLC Determination of Benzoxazolinone on Jeruju Plant (Acanthus Ilicifolius): Narrative Review

The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells

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