Karakterisasi  Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE &lt;br&gt;&lt;i&gt;[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]&lt;I&gt;

Mahasri, Gunanti; Fajriah, Ulia; Subekti, Sri

doi:10.20473/jipk.v2i1.11669

Cited by 5 publications

(2 citation statements)

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“…SDS-page analysis is carried out to detect protein molecules based on their molecular weight so that certain types of proteins are known (Roy and Kumar, 2014). According to Mahasri et al (2010), the determination of protein molecular weight can be done by calculating the RF (Retardation Factor), namely, the distance of the protein movement from the initial place divided by the distance of the color movement from the starting place of each protein band. The results of the calculation of the protein band Brachionus sp.…”

Section: Resultsmentioning

confidence: 99%

Exploration of Brachionus sp. Protein on the In Vivo Response of CD4 at Cantang Grouper (Epinephelus sp.) with VNN Infection (Viral Nervous Necrosis)

Martiningsih,

Yanuhar,

Musa

et al. 2023

JAFH

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This study examines the function of Brachionus sp. protein on the Cluster Differentiation-4 (CD4) immune system in cantang grouper infected with VNN. The purpose of this study was to determine the protein content of Brachionus sp. which has the potential as an antivirus and to find out the benefits of Brachionus sp. on the expression of CD4 as an indicator of increased fish body defense system against VNN. Brachionus sp. itself contains three protein bands with molecular weights of 122.73 kDa, 75.49 kDa, and 13.77 kDa, which are expected to increase the immune system against VNN on cantang grouper. Protein injection of Brachionus with doses of 35 µl, 105 µl and 170 µl /150 g cantang grouper, showed that the lowest decrease was at a dose of 105 µl/150 gram cantang groupers.

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Section: Resultsmentioning

confidence: 99%

Exploration of Brachionus sp. Protein on the In Vivo Response of CD4 at Cantang Grouper (Epinephelus sp.) with VNN Infection (Viral Nervous Necrosis)

Martiningsih,

Yanuhar,

Musa

et al. 2023

JAFH

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“…The sample is taken with a pipette and put into an Eppendorf. Protein was used as a marker with a molecular weight of 11-245 kDa [20]. The marker and sample were added by loading or reducing sample buffer (RSB) (1:1) and heated at 100˚C for 5 minutes.…”

Section: ) Injection and Sample Runningmentioning

confidence: 99%

Characteristics of Coagulants from Moringa Seed Extract (Moringa oleifera) with Microwave-Assisted Extraction (MAE) Method

Putri

Manab²,

Awwaly³

2022

AFSJ

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Moringa seed is an alternative coagulant in various industrial fields, generally used for water purification and cheese making. In addition, to obtain the required chemical compounds, Moringa seed can be extracted by conventional or modern methods. This research was conducted to obtain the coagulant characteristics of Moringa oleifera extracts using the Microwave-Assisted Extraction (MAE) method with different extraction times. This research was conducted at the Animal Products Laboratory, Faculty of Animal Science, and Biochemical Laboratory, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, between September 2021 and November 2022. The research material used was Moringa seed powder with different extraction times, designed by 4 treatments and 4 replications consisting of P0: maceration for 48 hours (control), P1: extraction for 6 minutes, P2: extraction for 9 minutes, and P3 extractions for 12 minutes with the MAE method. The variables tested were protein content, protein profile (SDS PAGE), milk clotting activity (MCA), and caseinolytic activity (CA), also the ratio of MCA to CA. The research method was a laboratory experiment analyzed by Analysis of Variance (ANOVA) using Duncan’s Multiple Range Test (DMRT) to determine if there were differences. The result showed a highly significant difference (P<0.01) in protein content, milk clotting activity (MCA), and caseinolytic activity (CA). However, there was no significant difference (P>0.05) in the ratio of MCA to CA. The characteristics of Moringa seed extract in this study were protein content ranging from 0.979-1.022 mg/ml, milk clotting activity (MCA) 3.837-3.999 SU/ml, caseinolytic activity (CA) 2.308-2.455 U/ml, and the ratio of MCA to CA 1.627-1.663. In conclusion, the best treatment for Moringa extraction time is 6 minutes with the MAE method, which can be used as a coagulant in the manufacture of mozzarella cheese.

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Antimicrobial potency of catfish (Clarias gariepinus) mucus

Widiyani,

Astuti,

Azizah

et al. 2024

Proceedings of the 2nd International Interdisciplinary Scientific Conference “Digitalization and Sustainability for Development

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Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE <br><i>[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]<I>

Cited by 5 publications

References 0 publications

Exploration of Brachionus sp. Protein on the In Vivo Response of CD4 at Cantang Grouper (Epinephelus sp.) with VNN Infection (Viral Nervous Necrosis)

Exploration of Brachionus sp. Protein on the In Vivo Response of CD4 at Cantang Grouper (Epinephelus sp.) with VNN Infection (Viral Nervous Necrosis)

Characteristics of Coagulants from Moringa Seed Extract (Moringa oleifera) with Microwave-Assisted Extraction (MAE) Method

Antimicrobial potency of catfish (Clarias gariepinus) mucus

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