KARAKTERISASI SIDIK JARI DNA ISOLAT Corynespora cassiicola YANG BERASAL DARI BERBAGAI SENTRA PERKEBUNAN KARET INDONESIA

Oktavia, Fetrina; Munir, Misbakhul; Suryaningtyas, Heru; Kuswanhadi, Kuswanhadi

doi:10.22302/jpk.v29i2.244

Cited by 1 publication

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“…The size of DNA product bands amplification ranges from 263-1200 bp for the three different primers . Polymorphism is amplification description that obtained from difference in DNA fragment studied and scoredits different sequence to show any variation (Oktavia, Munir, Suryatingtyas, & Kuswanhadi, 2011). The number of polymorphic DNA bands in genetic diversity analysis influences the diversity ona population, because the number of polymorphic DNA bands will be more capable to clearly describe the situation of plant genomes and reduce the bias due to the nonrepresentation of the genome parts of (Kawengian, Lengkong, & Mandang, 2016).…”

Section: Resultsmentioning

confidence: 99%

Genetic Characterization of Maize Kisar Var. Kuning Genjah and Maize Var. Bisi-II-Hibrida Based On Molekular RAPD Marker

Berhitu

Papilaya

Seumahu

2019

ATJ

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The variety of maize in Southwest Maluku (MBD) has an ability to grow in the extremely dry land, especially at Kisar island, because those areas were dominated by dry land. Dry resistance very useful to be used for genetical characterization in order to provide accurate data about the character of local maize Kisar var. Kuning Genjah. To identify the characteristics at the genome level of this local maize variety, one of the molecular methods that can be applied was RAPD molecular method. The advantage of the RAPD marker is it's technically simpler and fast in testing, does not require DNA sequence information, hence this marker becomes widely used, it only requires a small sample of DNA, the primary is commercially available and does not use radioactive compounds. The aim of this study was to find out the bands profile that has the potential to be used as a marker of dry resistance. The method applied was RAPD (Random Amplified Polymorphic DNA) using OPA-02, OPA-07, and OPC-12 primers and produces 22 DNA bands. The measurement of bands by estimating the molecular weight based on marker exponential regression. The polymorphic percentage was 90.9% between var. Kuning genjah and var. BIZI-II-Hybrid. The percentage of polymorphism showed the potential of bands that can be used as molecular markers for markers of dry resistance that can be utilized in plant breeding

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Section: Resultsmentioning

confidence: 99%