Carp (Cyprinus carpio) has the potential which is not only consumed from flesh as an edible portion but it is also able to be utilized from waste. One of waste is the scales of the carp known potentially contain of collagens. Micro-collagen has been extensively applied in various fields which were health and cosmetics. The problem to find the supply of collagens from non-halal animal sources and prone to infectious diseases is the fundamental consideration of this research to be undertaken in order to discover alternative sources of them. It was aimed at production and characterization of micro-collagen by utilizing carp scales waste. The stages of the proximate test, deproteinization, extraction, analysis, and characterization were series of processes to acquire collagen. The extraction results found that the yield of collagen extracted from carp scales waste was 8.62% with a yellowish-white color. Physical characterization of collagen obtained was pH of 6.59. The maximum of UV absorption at a wave length of 268nm was originated from the structure of collagen fibrils with amide bonds of A, B, I, II, and III. Furthermore, the characterization of micro-collagen showed a particle size distribution from the smallest particles which was 668 – 1581nm with the highest intensity at a particle size of 1146 nm according to PSA analysis and corresponding with the morphology of micro-collagen through visualization using SEM. It indicates that the carp scales waste have the potential to be used as an alternative source to find supply micro-collagen.