Karyological Status of Buccal Epithelial Cells of Miners With Occupational Lung Pathologies

Meyer, A. V.; Tolochko, T. A.; Litvin, A. V.; Минина, В. И.; Kulemin, Yu. E.

doi:10.18821/0016-9900-2018-97-3-220-225

Cited by 4 publications

(2 citation statements)

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“…It should be noted that most of the works devoted to the study of genotoxic effects using the BMCyt assay in miners are related to coal mining [54,68,69,85,86]. In these works, the authors showed a significant increase in the frequency of MN in buccal epithelium cells in coal miners.…”

Section: Discussionmentioning

confidence: 98%

“…However, a number of authors explain such nuclear anomalies as a result of genotoxic effects [46][47][48], epigenetic processes [49], or as a result of the budding of the nucleus during the S-phase [50]. At the same time, a number of researchers propose to consider their formation as a result of cytogenetic disorders since a significant increase in the frequency of nuclear protrusions was shown during exposure to mutagens [51][52][53][54]. At the same time, the authors suggest that the onset of nuclear buds may be associated with the elimination of amplified DNA and DNA repair processes [45,49,50,55].…”

Section: Discussionmentioning

confidence: 99%

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Genetic Damage in Workers from the Rare Metal Ore Production Region

2019

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The aim of this study was to assess the genotoxic and cytotoxic effects that occur in miners working underground in rare metal ore mines at ionizing radiation levels within the maximum permissible concentration (MPC) standards using the buccal micronucleus cytome (BMCyt) assay. At the same time, we attempted to identify possible relationships between cytogenetic abnormalities in the buccal epithelium and such physiological indicators as cardiodynamics and the body mass index. The study involved a total of 269 individuals, 236 miners working underground in production related to the mining and enrichment of loparite ores and 33 non-exposed individuals (Russia, Murmansk region). Buccal micronucleus cytome assay and heart rate variability parameters were used. Micronucleus frequency was revealed to be a higher in the exposed group (8.3 ± 1.08‰) than in the non-exposed group (3.3 ± 0.49‰). The repair index showed a statistically significant increase in the non-exposed group (14.9 ± 2.90) compared with the exposed group (6.1 ± 0.74). The frequency of nuclear protrusions correlates significantly with the body mass index in the young miners (≤30 years (r = 0.45)). There is a negative relationship between the micronucleus frequency and the level of organism adaptation (r = −0.24), the vegetative regulation rate (r = −0.20) and the psycho-emotional state (r = −0.30) for miners older than 30 years. The results testify to the possible induction of clastogenic effects and a decrease in the level of reparation in the buccal epithelium cells due to the exposure of a various of production factors in the extraction of loparite ore.

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