Karyology of the toadfish Porichthys plectrodon (Jordan and Gilbert, 1882) (Batrachoididae) from Margarita Island, Venezuela

Nirchio, Mauro; Fenocchio, Alberto Sérgio; Swarça, Ana Cláudia; Pérez, Julio E.

doi:10.1007/s00227-004-1422-7

Cited by 9 publications

(9 citation statements)

References 13 publications

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“…Cytogenetic studies in the Batrachoididae family are few, and classic cytogenetic techniques have been applied, mainly to determine chromosome number and karyotype in Porichtys notatus (Gold et al 1980), Batrachoides pacifici (Nirchio et al 2001), Porichtys porosissimus (Brum et al 2001), Amphichtys cryptocentrus (Nirchio et al 2002), Batrachoides manglae (Nirchio et al 2002), Thalassophryne maculosa (Nirchio et al 2002(Nirchio et al , 2004a, Halobatrachus didactylus (Palazón et al 2003;Merlo et al 2007), Porichthys plectrodon (Nirchio et al 2004b) and Halophryne trispinosus (Magtoon and Donsakul 2008). However, studies using more advanced cytogenetic techniques are very scarce.…”

Section: Introductionmentioning

confidence: 99%

“…Double-color FISH with digoxigenin-labelled 18S rDNA probe (green, arrow heads) and rhodamine-labelled 5S rDNA probe (red, arrows): a Amphichthys cryptocentreus, b Batrachoides manglae, c Porichthys plectrodon, and d Thalassophryne maculosa as follows: A. cryptocentrus (2n = 46) 4M, 2SM, 40A(Nirchio et al 2002), B. manglae, (2n = 46) 6M, 6SM, 34A(Nirchio et al 2002), P. plectrodon (2n = 44) 8M, 10SM, 6ST, 20A(Nirchio et al 2004b), and. T. maculosa (2n = 46) 12M, 6SM, 20ST, 8A(Nirchio et al 2004a).…”

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confidence: 99%

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Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family

et al. 2010

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In the present study dual-colour fluorescence in situ hybridization (FISH) was performed to study the chromosomal distribution of 18S and 5S rDNAs, (GATA)(n) and 5S rDNA, and U2 snRNA and 18S rDNA in four species of Batrachoididae family: Amphichthys cryptocentrus, Batrachoides manglae, Porichthys plectrodon and Thalassophryne maculosa. The 18S rDNA signals were present in only one pair of chromosomes in all the four Batrachoididae species. The 5S rDNA was mapped on one pair of chromosomes, except in B. manglae, which showed a hybridization signal in two pairs. The two ribosomal genes are located on different chromosome pairs, except in A. cryptocentrus, in which they appear co-located. In all the cases, the (GATA)(n) probe produced disperse hybridization signals in all four species. The U2 snRNA signals appear very widely scattered in A. cryptocentrus, P. plectrodon, but show a degree of clustering in a specific chromosome pair in B. manglae. In T. maculosa, they are thinly dispersed and strong hybridization signals are observed co-located to the 18S rDNA-bearing chromosomes. Finally, a double-colour FISH with U2 snRNA and 5S rDNA probes was performed in B. manglae, and this showed that these genes were not co-located. These results have been compared with those from another Batrachoididae species, and evolutive processes of these species are discussed.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family

et al. 2010

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“…Some morphological and size features of the first chromosome pair seem to indicate a homeologous condition for the first metacentric pair within this family, although the presence of more conspicuous heterochromatic blocks at pericentromeric positions was reported in P. plectrodon (Nirchio et al, 2004b).…”

Section: Discussionmentioning

confidence: 76%

“…Such distribution pattern and amount of heterochromatin is similar to that reported in T. maculosa (Nirchio et al, 2004a). On the other hand, the cytogenetic data described for Thalassophryne differ from the pattern observed in the genus Porichthys (Brum et al, 2001;Nirchio et al, 2004b), i.e., large heterochromatic blocks restricted to some chromosome pairs, besides conspicuous pericentromeric heterochromatic segments in the first and second chromosome pairs. Such heterochromatin distribution has been referred to as vestiges of the Robertsonian fusions originating the two largest metacentric pairs in the karyotype (Brum et al, 2001).…”

Section: Discussionmentioning

confidence: 81%

“…Chromosomal measurements in T. nattereri demonstrated that the largest chromosome pair shows an average size of 5.5 µm, or 7.49% of the total karyotype length, while, in Porichthys plectrodon (Nirchio et al, 2004b), the first pair represents a relatively larger portion of the total karyotype length (13.5%) but the same absolute size (5.48 µm). Some morphological and size features of the first chromosome pair seem to indicate a homeologous condition for the first metacentric pair within this family, although the presence of more conspicuous heterochromatic blocks at pericentromeric positions was reported in P. plectrodon (Nirchio et al, 2004b).…”

Section: Discussionmentioning

confidence: 99%

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Karyoevolution of the toadfish Thalassophryne nattereri (Batrachoidiformes: Batrachoididae)

Costa

Molina²

2009

Genet. Mol. Res.

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ABSTRACT. The Batrachoididae includes some venomous brackish and marine fish found in the Atlantic, Indian and Pacific oceans. This family is composed of 69 species, distributed among 19 genera. Species of the genus Thalassophryne have been reported along the coast of Rio Grande do Norte (Brazil); T. nattereri has been responsible for a large number of human injuries. Little is known about the cytogenetic features of this family. We made a karyotypic characterization of T. nattereri collected from the estuary of the Apodi/Mossoró River, using conventional Giemsa staining, C-banding and silver nitrate-nucleolar organizer region technique. There was a modal diploid value of 2n = 46 chromosomes (8m + 8sm + 24st + 6a; fundamental number = 86). Single ribosomal sites were detected in the terminal region on short arms of a subtelocentric pair (19th). Heterochromatin segments were preferentially located over centromeric regions in some chromosome pairs. Pericentric inversions and Robertsonian rearrangements seem to have played a major role in karyotype evolution within this genus of toadfish.

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Cytogenetic analysis of three species of the genus Haemulon (Teleostei: Haemulinae) from Margarita Island, Venezuela

et al. 2006

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This paper describes the karyotype analysis of Haemulon aurolineatum, Haemulon bonariensis and Haemulon plumierii, by Giemsa staining, C-banding, Ag-staining and fluorescent in situ hybridization (FISH), to locate the 18S and 5S rRNA genes. Diploid modal count in the three species was 2n = 48 acrocentric elements. Except for pair 24, which exhibited an unmistakable secondary constriction in all three species, it was not possible to classify them as homologous to each other because differences in chromosome size were too slight between adjacent pairs within a size-graded series. Ag-NOR clusters were located in pair 24 in the three species with signal located on the secondary constriction of these chromosomes. C-banding demonstrated that the three species share the same distribution pattern of the constitutive heterochromatin with centromeric heterochromatic blocks in the 23 chromosome pairs and a pericentromeric block in pair 24 which is coincident with the NORs. FISH experiments showed that 18S rDNA sequences were located coincident with the Ag-NOR site in the three species; however, differences in both the number and chromosome distribution of 5S-rDNA cluster were detected among them. Our data suggest that chromosome evolution of Haemulon has been preserved from major changes in the karyotypic macrostructure, whereas microstructural changes have occurred.

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Karyology of the toadfish Porichthys plectrodon (Jordan and Gilbert, 1882) (Batrachoididae) from Margarita Island, Venezuela

Cited by 9 publications

References 13 publications

Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family

Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family

Karyoevolution of the toadfish Thalassophryne nattereri (Batrachoidiformes: Batrachoididae)

Cytogenetic analysis of three species of the genus Haemulon (Teleostei: Haemulinae) from Margarita Island, Venezuela

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