Karyotype analysis of Lablab purpureus (L.) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes

She, Chao-Wen; XiangHui, Jiang

doi:10.17221/32/2015-cjgpb

Cited by 27 publications

(19 citation statements)

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“…Another prominent feature of the two Canavalia genomes was the non-rDNA GC-rich heterochromatin in all centromeres (highlighted by CPD staining) ( She et al 2006 ). Centromeric, pericentromeric, or proximal non-rDNA GC-rich heterochromatin have been observed in many Phaseoleae, including Psophocarpus tetragonolobus A. P. de Candolle, 1825 ( Chaowen et al 2004 ), four cultivated Phaseolus (Linnaeus, 1754) species ( Bonifácio et al 2012 ), seven cultivated Vigna (Savi, 1824) species ( She et al 2015 ), Lablab purpureus (Linnaeus, 1763) Sweet, 1826 ( She and Jiang 2015 ), and Crotalaria (Linnaeus, 1753) species from two sections of the tribe Crotalarieae ( Mondin and Aguiar-Perecin 2011 ) which is a branch of the Genistoid clade ( LPWG 2013 ). A recent multilocus phylogenetic analysis reestablished the tribe Diocleae as a branch of the Phaseoloid (Millettioid) clade, which includes the Canavalia and two other clades ( Queiroz et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Detailed karyotypes displaying chromosome morphology, heterochromatin distribution, and location of repetitive DNA sequences and bacterial artificial chromosome ( BAC ) have been constructed for many plant species. These are used to reveal chromosome-level genome organization, investigate the evolutionary relationships among related species, and integrate genetic and physical maps ( Fuchs et al 1998 , Moscone et al 1999 , Hasterok et al 2001 , de Moraes et al 2007 , Hamon et al 2009 , Robledo et al 2009 , Fonsêca et al 2010 , Chacón et al 2012 , She et al 2015 , She and Jiang 2015 , Zhang et al 2015 , Kirov et al 2016 ). Karyotype analysis is often hampered by limitations in the ability to identify individual chromosomes due to a lack of markers.…”

Section: Introductionmentioning

confidence: 99%

“…The NTS sequences of 5S rDNA have been used to study phylogenetic relationships among infrageneric taxa ( Liu et al 2003 ). The distribution patterns of rRNA genes revealed by FISH can be used as karyotype markers ( Moscone et al 1999 , Hasterok et al 2001 , Chacón et al 2012 , She et al 2015 , She and Jiang 2015 , Kirov et al 2016 ). In a phylogenetic context, interpreting the changes in the number and location of rDNA loci in related species facilitates the understanding of the mechanisms and directions of chromosomal changes and their impact on plant evolution (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cytogenetic characterization and comparison of the two cultivated Canavalia species (Fabaceae)

She¹,

Lin²,

XiangHui³

2017

CCG

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The two cultivated Canavalia (Adanson, 1763) species, Canavalia gladiata (N. J. von Jacquin, 1788) A. P. de Candolle, 1825 and Canavalia ensiformis (Linnaeus, 1753) A. P. de Candolle, 1825 are closely related based on morphological and molecular phylogenetic data. However, the similarities and differences in genome organization between them have not been evaluated at molecular cytogenetic level. Here, detailed karyotypes of both species were constructed using combined PI and DAPI (CPD) staining, rDNA-FISH and self-genomic in situ hybridization (sGISH). For further comparison, comparative genomic in situ hybridization (cGISH) and sequence analysis of 5S rDNA were applied. Their chromosomes were accurately identified by sGISH and rDNA-FISH signals. Both species had the karyotype formula 2n = 22 = 18m + 4m-SAT, but the karyotype of C. ensiformis was shorter and more asymmetric than that of C. gladiata. They displayed similar CPD bands at all 45S rDNA sites and centromeres. C. gladiata had ten centromeric 5S rDNA loci and two SC (secondary constriction)-associated 45S rDNA loci. C. ensiformis had nine centromeric and one interstitial 5S loci, two SC-associated and one proximal 45S loci. Their sGISH signal patterns displayed both basic similarities and distinct differences. Reciprocal cGISH generated prominent signals in all pericentromeric regions and 45S sites. There was lower level of sequence identity of the non-transcribed spacer between their 5S rDNA repeats. These data confirmed the evolutionary closeness between C. gladiata and C. ensiformis and demonstrated obvious differentiation between their genomes, and supported the opinion that C. ensiformis is more advanced in evolution than C. gladiata.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular cytogenetic characterization and comparison of the two cultivated Canavalia species (Fabaceae)

She¹,

Lin²,

XiangHui³

2017

CCG

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“…Prometaphase chromosomes are effective and preferable for cytogenetic analyses and identification of individual chromosomes because the chromosomes are easily distinguishable due to the uneven condensation of chromatin fibers along chromosomes [ 4 ]. FISH using prometaphase chromosomes has been successfully applied in studies involving Brassica [ 5 ], rice [ 6 – 8 ], Catharanthus roseus [ 9 ], and Lablab purpureus [ 10 ]. However, suitable methods to induce prometaphase chromosomes in other plants have been poorly investigated.…”

Section: Introductionmentioning

confidence: 99%

An improved method for inducing prometaphase chromosomes in plants

et al. 2018

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BackgroundDetailed karyotyping using metaphase chromosomes in melon (Cucumis melo L.) remains a challenge because of their small chromosome sizes and poor stainability. Prometaphase chromosomes, which are two times longer and loosely condensed, provide a significantly better resolution for fluorescence in situ hybridization (FISH) than metaphase chromosomes. However, suitable method for acquiring prometaphase chromosomes in melon have been poorly investigated.ResultsIn this study, a modified Carnoy’s solution II (MC II) [6:3:1 (v/v) ethanol: acetic acid: chloroform] was used as a pretreatment solution to obtain prometaphase chromosomes. We demonstrated that the prometaphase chromosomes obtained using the MC II method are excellent for karyotyping and FISH analysis. We also observed that a combination of MC II and the modified air dry (ADI) method provides a satisfactory meiotic pachytene chromosome preparation with reduced cytoplasmic background and clear chromatin spreads. Moreover, we demonstrated that pachytene and prometaphase chromosomes of melon and Abelia × grandiflora generate significantly better FISH images when prepared using the method described. We confirmed, for the first time, that Abelia × grandiflora has pairs of both strong and weak 45S ribosomal DNA signals on the short arms of their metaphase chromosomes.ConclusionThe MC II and ADI method are simple and effective for acquiring prometaphase and pachytene chromosomes with reduced cytoplasm background in plants. Our methods provide high-resolution FISH images that can help accelerate molecular cytogenetic research in plants.

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“…The fresh pods are the harvestable economic product in dolichos bean. It is predominantly a selfpollinated crop (Ayyangar and Nambiar, 1935) with 2n=2x=22 chromosomes (She and Jiang, 2015) with a genome size of 367 Mbp (Iwata et al, 2013). Handling of segregating generations derived from crosses involving deliberately selected parents followed by pedigree selection is the most widely used breeding method for genetic improvement of fresh pod yield and its component traits in dolichos bean .…”

Section: Introductionmentioning

confidence: 99%

Cross Legume Species/Genera Transferability of SSR Markers and their Utility in Assessing Polymorphism among Advanced Breeding Lines in Dolichos Bean (Lablab purpureus L.)

Shivakumar¹,

Ramesh²,

Rao³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Karyotype analysis of Lablab purpureus (L.) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes

Cited by 27 publications

References 26 publications

Molecular cytogenetic characterization and comparison of the two cultivated Canavalia species (Fabaceae)

Molecular cytogenetic characterization and comparison of the two cultivated Canavalia species (Fabaceae)

An improved method for inducing prometaphase chromosomes in plants

Cross Legume Species/Genera Transferability of SSR Markers and their Utility in Assessing Polymorphism among Advanced Breeding Lines in Dolichos Bean (Lablab purpureus L.)

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