Karyotype and Tumorigenicity in Nude Mice of Human Melanoma Cell Lines

Expression of cell surface glycoproteins in human melanoma cell lines with different tumorigenic properties

“…Recent studies performed in our laboratory have shown that human malignant melanoma cell lines could be individually characterized by their ability to grow subcutaneously in nude mice (Jacubovich et al, 1984 a,b) and that cell lines with high or low tumorigenicity could be distinguished by specific features in their karyotypes (Bertrand et al, 1984) and polyamine metabolism (Thomasset et al, 1982a,b). Hence, human melanoma cell lines with known tumorigenicity may represent a model system to study the relationships between cell surface characteristics and expression of the malignant phenotype in human tumor cells.…”

Tumourigenic phenotypes of human melanoma cell lines in nude mice determined by an active antitumour mechanism

“…In addition, at least for one PCTL which has been studied in greater detail, this phenotype seemed to be stable even after changing in vitro culture conditions and each cell clone derived from it also displayed the same tumourigenic phenotype. The definition of a xenografting phenotype has not been previously proposed to our knowledge mainly because the tumour cell inoculum used in other reports varied greatly from 106 to 20 x 106 cells (Fogh et al, (Bertrand et al, 1984), polyamine metabolism (Thomasset et al, 1982) and cell surface glycoconjugates (Berthier-Vergnes et al, submitted for publication). Interestingly, the karotypic and biochemical phenotypes of HCML seemed to correlate with their xenografting phenotypes.…”

“…Electronmicroscopy studies of nude mouse tumours and of cells recultured from tumours showed ultrastructural characteristics of melanocytes. Karyotypes of cells recultured from nude mouse tumours have been shown to be human and to have retained marker chromosomes characterizing the HMCL prior to heterotransplantation (Bertrand et al, 1984). Antiasialo GM1 treatment Rabbit antiasialo GM1 serum was a generous gift from M. Iwamori.…”