Karyotype Divergence and Spreading of 5S rDNA Sequences between Genomes of Two Species: Darter and Emerald Gobies (&lt;b&gt;&lt;i&gt;Ctenogobius&lt;/i&gt;&lt;/b&gt;, Gobiidae)

Lima-Filho, Paulo Augusto de; Bertollo, L.A.C.; Cioffi, Marcelo de Bello; Costa, Gideão Wagner Werneck Félix da; Molina, Wagner Franco

doi:10.1159/000360492

Cited by 17 publications

(23 citation statements)

References 57 publications

(52 reference statements)

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“…Although collinear and equilocal arrangements of 5S and 18S ribosomal genes are equally rare in fishes [Fujiwara et al, 1998], varying levels of chromosomal distribution have been identified in the genes of a number of fish species at times with greater variability in the distribution of 45S rDNA sites [Mantovani et al, 2005] and sometimes of 5S rDNA sites [Affonso and Galetti, 2005;Lima-Filho et al, 2014]. This condition is also observed in R. canadum , in both the differential frequency of these gene families and the colocalized or independent position in diverse chromosomes.…”

Section: Ribosomal 5s and 18s Chromosomal Mappingmentioning

confidence: 99%

Unusual Dispersion of Histone Repeats on the Whole Chromosomal Complement and Their Colocalization with Ribosomal Genes in Rachycentron canadum (Rachycentridae, Perciformes)

Costa

Cioffi²,

Bertollo³

et al. 2014

Cytogenet Genome Res

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Rachycentron canadum, the only representative of the family Rachycentridae, has been the focus of biotechnological interest due to its significant potential in marine fish farming. The chromosome set of this species has been widely investigated with respect to the location of genes and multigene families. A FISH analysis was performed using 4 multigene families as probes, represented by 5S and 18S ribosomal genes and histones H2B-H2A and H3. Earlier data suggested that differential replication of heterochromatin could be partially associated with functional genes. Indeed, our results showed that the DNA contained in heterochromatic regions of R. canadum contains 5S and 18S ribosomal genes as well as the gene sequences of histones H2B-H2A and H3, which were colocalized. The distribution of H3 sequences in all heterochromatic regions, except in 13q, could indicate an important evolutionary role for this class of repetitive sequences. Besides, the presence of chromosome regions bearing multifunctional repetitive sequences formed by H2B-H2A/H3/18S rDNA and H2B-H2A/H3/5S rDNA clusters was demonstrated for the first time in fishes. The implications of differential histone gene extension and its functionality in the karyotype of R. canadum remain unknown.

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Section: Ribosomal 5s and 18s Chromosomal Mappingmentioning

confidence: 99%

Unusual Dispersion of Histone Repeats on the Whole Chromosomal Complement and Their Colocalization with Ribosomal Genes in Rachycentron canadum (Rachycentridae, Perciformes)

Costa

Cioffi²,

Bertollo³

et al. 2014

Cytogenet Genome Res

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“…However, a large number of GC-rich sites were observed in the centromeric and terminal regions of various chromosomes in Ctenogobius smaragdus and C. boleosoma [Lima-Filho et al, 2014] and previously in Neogobius eurycephalus [Ene, 2003]. In the latter species, where karyomorphs with 2n = 30, 31 and 32 derived from Robertsonian fusions, the presence of GC-rich sites is a possible promoter of these rearrangements.…”

Section: Heterochromatin Distribution and Rdna Sites In Gobionellusmentioning

confidence: 80%

“…Together with these variations, modifications in the heterochromatin composition and distribution pattern of repetitive sequences in the karyotype were also observed in several gobiid species [Caputo et al, 1997;Lima-Filho et al, 2014]. The propensity for centric fusions and fissions in the genomes of representatives of this family has been tentatively explained by the occurrence of specific 338 DNA sequences [Canapa et al, 2002], possibly in the centromeres or telomeres, as found in other vertebrate groups [Slijepcevic et al, 1997].…”

Section: Karyotype Diversification and Sex Chromosomesmentioning

confidence: 88%

“…In the latter species, where karyomorphs with 2n = 30, 31 and 32 derived from Robertsonian fusions, the presence of GC-rich sites is a possible promoter of these rearrangements. In some gobies, such as C. smaragdus [Lima-Filho et al, 2014], Gobius paganellus , G. niger , G. cobitis , and Zosterisessor ophiocephalus [Caputo et al, 1997], it has been suggested that differences in the heterochromatin composition, as exhibited by species of Gobionellus, could favor the occurrence and fixation of chromosomal rearrangements.…”

Section: Heterochromatin Distribution and Rdna Sites In Gobionellusmentioning

confidence: 99%

“…Such a remarkable karyotypic diversity in this group [Vasil'ev and Grigoryan, 1993;Galvão et al, 2011;LimaFilho et al, 2014] reveals the involvement of different chromosomal rearrangements, strongly contrasting with the conserved pattern present in numerous families of the order Perciformes [Molina, 2007;Motta-Neto et al, 2011;Calado et al, 2013]. This evolutionary dynamism includes a high degree of polymorphism associated with Robertsonian translocations, tandem fusions and pericentric inversions Thode et al, 1985;Amores et al, 1990;Caputo et al, 1997;Ene, 2003], as well as interspecific karyotypic diversifications [Lee et al, 1987], especially significant variations in the content of repetitive sequences among species [Lima-Filho et al, 2014].…”

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confidence: 99%

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Chromosomal Mapping of Repetitive DNAs in Gobionellus oceanicus and G. stomatus (Gobiidae; Perciformes): A Shared XX/XY System and an Unusual Distribution of 5S rDNA Sites on the Y Chromosome

Lima-Filho¹,

Amorim²,

Cioffi³

et al. 2014

Cytogenet Genome Res

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With nearly 2,000 species, Gobiidae is the most specious family of the vertebrates. This high level of speciation is accompanied by conspicuous karyotypic modifications, where the role of repetitive sequences remains largely unknown. This study analyzed the karyotype of 2 species of the genus Gobionellus and mapped 18S and 5S ribosomal RNA genes and (CA)₁₅ microsatellite sequences onto their chromosomes. G. oceanicus (2n = 56; ♂ 12 metacentrics (m) + 4 submetacentrics (sm) + 1 subtelocentric (st) + 39 acrocentrics (a); ♀ 12m + 4sm + 2st + 38a) and G. stomatus (2n = 56; ♂ 20m + 14sm + 1st + 21a; ♀ 20m + 14sm + 2st + 20a) possess the highest diploid chromosome number among the Gobiidae and have different karyotypes. Both species share an XX/XY sex chromosome system with a large subtelocentric X and a small acrocentric Y chromosome which is rich in (CA)₁₅ sequences and bears 5S rRNA sites. Although coding and noncoding repetitive DNA sequences may be involved in the genesis or differentiation of the sex chromosomes, the exclusive presence of 5S rDNA sites on the Y, but not on the X chromosome of both species, represents a novelty in fishes. In summary, the karyotypic differences, as well as new data on the sex chromosome systems in these 2 Gobiidae species, confirm the high chromosomal dynamism observed in this family.

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Evolutionary trends in animal ribosomal DNA loci: introduction to a new online database

et al. 2017

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Ribosomal DNA (rDNA) loci encoding 5S and 45S (18S-5.8S-28S) rRNAs are important components of eukaryotic chromosomes. Here, we set up the animal rDNA database containing cytogenetic information about these loci in 1343 animal species (264 families) collected from 542 publications. The data are based on in situ hybridisation studies (both radioactive and fluorescent) carried out in major groups of vertebrates (fish, reptiles, amphibians, birds, and mammals) and invertebrates (mostly insects and mollusks). The database is accessible online at www.animalrdnadatabase.com. The median number of 45S and 5S sites was close to two per diploid chromosome set for both rDNAs despite large variation (1–74 for 5S and 1–54 for 45S sites). No significant correlation between the number of 5S and 45S rDNA loci was observed, suggesting that their distribution and amplification across the chromosomes follow independent evolutionary trajectories. Each group, irrespective of taxonomic classification, contained rDNA sites at any chromosome location. However, the distal and pericentromeric positions were the most prevalent (> 75% karyotypes) for 45S loci, while the position of 5S loci was more variable. We also examined potential relationships between molecular attributes of rDNA (homogenisation and expression) and cytogenetic parameters such as rDNA positions, chromosome number, and morphology.Electronic supplementary materialThe online version of this article (10.1007/s00412-017-0651-8) contains supplementary material, which is available to authorized users.

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Karyotype Divergence and Spreading of 5S rDNA Sequences between Genomes of Two Species: Darter and Emerald Gobies (<b><i>Ctenogobius</i></b>, Gobiidae)

Cited by 17 publications

References 57 publications

Unusual Dispersion of Histone Repeats on the Whole Chromosomal Complement and Their Colocalization with Ribosomal Genes in <b><i>Rachycentron canadum</i></b> (Rachycentridae, Perciformes)

Unusual Dispersion of Histone Repeats on the Whole Chromosomal Complement and Their Colocalization with Ribosomal Genes in <b><i>Rachycentron canadum</i></b> (Rachycentridae, Perciformes)

Chromosomal Mapping of Repetitive DNAs in <b><i>Gobionellus oceanicus</i></b> and <b><i>G. stomatus</i></b> (Gobiidae; Perciformes): A Shared XX/XY System and an Unusual Distribution of 5S rDNA Sites on the Y Chromosome

Evolutionary trends in animal ribosomal DNA loci: introduction to a new online database

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