Karyotyping of human chondrocytes in scaffold-assisted cartilage tissue engineering

Trimborn, Marc; Endres, Michaela; Bommer, Christiane; Janke, Una; Krüger, Jan-Philipp; Morawietz, Lars; Kreuz, Peter; Kaps, Christian

doi:10.1016/j.actbio.2011.12.024

Cited by 11 publications

(15 citation statements)

References 49 publications

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“…In contrast, from another study, eight post-mortem specimens from old donors analyzed by GTG-banding showed high frequency of aneuploidies 8 . Predominantly loss of one gonosome and gains of chromosomes 5, 7, 8 and gonosomes were detected.…”

Section: Autosomal Stabilitymentioning

confidence: 67%

“…Interestingly, all these aberrations were already present in the first passage directly after the establishment of the cell culture, suggesting their presence in the original cartilage tissue. In the same study, five samples from younger patients with cartilage damage showed mostly normal karyotypes with negligible single non-clonal events of numerical and structural aberrations 8 . These results are in concordance with past studies showing for a limited number of donors, that chondrocytes subjected to knee surgery showed a normal karyotype 9 and that even extensive expansion of the chondrocytes had no effect on chromosome number or structure 10 .…”

Section: Autosomal Stabilitymentioning

confidence: 72%

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Genomic chondrocyte culture profiling by array-CGH, interphase-FISH and RT-PCR

Stümm

Boger

Gaissmaier

et al. 2012

Osteoarthritis and Cartilage

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Section: Autosomal Stabilitymentioning

confidence: 67%

Section: Autosomal Stabilitymentioning

confidence: 72%

Genomic chondrocyte culture profiling by array-CGH, interphase-FISH and RT-PCR

Stümm

Boger

Gaissmaier

et al. 2012

Osteoarthritis and Cartilage

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“…PGA is a synthetic polymer successfully used in cartilage tissue engineering for its biocompatibility and mechanical properties (Trimborn et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Human platelet releasates combined with polyglycolic acid scaffold promote chondrocyte differentiation and phenotypic maintenance

et al. 2015

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In the present study, we aimed to demonstrate the differentiating properties of platelet-rich plasma releasates (PRPr) on human chondrocytes seeded on a polygtlycolic acid (PGA) 3D scaffold. Gene expression and biochemical analysis were carried out to assess the improved quality of our PGA-based cartilage constructs supplemented with PRPr. We observed that the use of PRPr as cell cultures supplementation to PGA-chondrocyte constructs may promote chondrocyte differentiation, and thus may contribute to maintaining the chondrogenic phenotype longer than conventional supplementation by increasing high levels of important chondrogenic markers (e.g. sox9, aggrecan and type II collagen), without induction of type I collagen. Moreover, our constructs were analysed for the secretion and deposition of important ECM molecules (sGAG, type II collagen, etc.). Our results indicate that PRPr supplementation may synergize with PGA-based scaffolds to stimulate human articular chondrocyte differentiation, maturation and phenotypic maintenance.

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“…93 We developed a 3D single cell culture system that exploits single 94 cell patterning with in situ matrix gelation, to create well-defined 95 was then employed to remove amino-groups on the exposed sur-113 face. A second surface modification of the exposed area was con- The cell suspension was 146 then adjusted to desirable seeding densities for different sized pat-147 terns, namely 2 Â 10 5 , 0.5 Â 10 5 and 0.2 Â 10 5 cells/ml for 20,40,148 and 80 lm micropatterns respectively. A patterned substrate was 149 then immersed in a cell suspension of appropriate density for 150 1-2 h, followed by removal of cell suspension and a gentle rinse 151 with fresh medium to remove loosely attached cells.…”

Section: Introductionmentioning

confidence: 99%

“…40, and 80 lm were prepared. The distance between two adja-253 cent circles was either 80 or 100 lm.…”

mentioning

confidence: 99%

A novel culture system for modulating single cell geometry in 3D

et al. 2015

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Dedifferentiation of chondrocytes during in vitro expansion remains an unsolved challenge for repairing serious articular cartilage defects. In this study, a novel culture system was developed to modulate single cell geometry in 3D and investigate its effects on the chondrocyte phenotype. The approach uses 2D micropatterns followed by in situ hydrogel formation to constrain single cell shape and spreading. This enables independent control of cell geometry and extracellular matrix. Using collagen I matrix, we demonstrated the formation of a biomimetic collagenous "basket" enveloping individual chondrocytes cells. By quantitatively monitoring the production by single cells of chondrogenic matrix (e.g. collagen II and aggrecan) during 21-day cultures, we found that if the cell's volume decreases, then so does its cell resistance to dedifferentiation (even if the cells remain spherical). Conversely, if the volume of spherical cells remains constant (after an initial decrease), then not only do the cells retain their differentiated status, but previously de-differentiated redifferentiate and regain a chondrocyte phenotype. The approach described here can be readily applied to pluripotent cells, offering a versatile platform in the search for niches toward either self-renewal or targeted differentiation.

show abstract

Karyotyping of human chondrocytes in scaffold-assisted cartilage tissue engineering

Cited by 11 publications

References 49 publications

Genomic chondrocyte culture profiling by array-CGH, interphase-FISH and RT-PCR

Genomic chondrocyte culture profiling by array-CGH, interphase-FISH and RT-PCR

Human platelet releasates combined with polyglycolic acid scaffold promote chondrocyte differentiation and phenotypic maintenance

A novel culture system for modulating single cell geometry in 3D

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