Katanin P60 and P80 in papillary thyroid carcinoma patients: Indicators for exacerbated tumor features and worse disease‐free survival

Abstract

Background This study aimed to explore the clinical implications of katanin P60 and P80 (katanin P60/P80) regarding their correlations with clinicopathological features and survival profiles in papillary thyroid carcinoma (PTC) patients. Methods Tumor tissue and paired adjacent tissue specimens were obtained from 172 PTC patients who underwent lobectomy or thyroidectomy. Besides, immunohistochemistry assay and immunoreactive (IR) score (multiplying staining intensity score by density score) were used to determine katanin P60/P80 expressions. According to IR score (from 0 ~ 12), katanin P60/P80 expressions were classified as low (IR score 0 ~ 3) and high (IR score 4 ~ 12) expressions. Results Both katanin P60/P80 expressions were highly expressed in tumor tissue compared with adjacent tissue. Besides, tumor katanin P60 expression positively correlated with tumor katanin P80 expression. Tumor katanin P60 high expression correlated with larger tumor size, extrathyroidal invasion, advanced pT stage, pN stage, and pTNM stage, while no correlation of tumor katanin P60 expression with age or gender was observed; tumor katanin P80 high expression correlated with advanced pN stage and pTNM stage, whereas there was no correlation of tumor katanin P80 expression with age, gender, tumor size, extrathyroidal invasion, or pT stage. Furthermore, both tumor katanin P60/P80 high expressions correlated with shorter accumulating disease‐free survival. As for overall survival (OS), neither tumor katanin P60 nor P80 expression correlated with OS. Conclusion Katanin P60/P80 measurement might assist with tumor management and prognosis surveillance in PTC patients.

Katanin subunits p60 and p80, potential biomarkers for papillary thyroid carcinoma to distinguish nodular goiter: STROBE

“…Several studies have shown increased katanin p60 or p80 expression in breast, prostate, and NSCLC tissues separately, [21–26] but the relationship between katanin p60 and p80 expression in these cancers has not been reported. In this study, we also found that katanin p60 levels were positively correlated with katanin p80 levels in PTC, including CPTC and FVPTC, which is consistent with a recently reported study [27] . To further confirm this, we overexpressed katanin p60 in human papillary thyroid carcinoma KTC-1 cells and found that overexpression of katanin p60 significantly increased katanin p80 expression.…”

“…In this study, we also found that katanin p60 levels were positively correlated with katanin p80 levels in PTC, including CPTC and FVPTC, which is consistent with a recently reported study. [ 27 ] To further confirm this, we overexpressed katanin p60 in human papillary thyroid carcinoma KTC-1 cells and found that overexpression of katanin p60 significantly increased katanin p80 expression. As a regulatory subunit, katanin p80 positively regulates the activity of katanin p60.…”

“…These results clearly show that katanin subunits p60 and p80 are not only highly expressed in cancer tissues, but also play a role in the proliferation, migration, and metastasis of cancer cells, and are related to the prognosis of cancer patients. Importantly, it has recently been reported that compared with adjacent PTC tissues, katanin p60 and 80 are highly expressed in tumor tissues and both were associated with lymph node metastasis [27] . However, in our study, although the expression of katanin p60 and p80 was found in PTC, not in NG, we only found a significant association between katanin p60 expression and lymph node metastasis in PTC, and this may be due in part to the benign nature of PTC relative to cancers of the prostate, breast, and NSCLC, or a fairly limited number of patients with PTC or 2 subtypes of PTC in our study.…”

“…[ 25 , 26 ] In particular, it has recently been reported that compared with adjacent PTC tissues, both are highly expressed in tumor tissues. [ 27 ] Interestingly, it has been reported that LAPSER1 , a putative tumor suppressor, is involved in cytokinesis via its interaction with katanin p80. Consequently, since disruption of LASPER1 has been shown to cause genetic instability and cancer, [ 28 ] this indicates that the dysregulation of katanin p80 may result in tumorigenesis.…”

The Mammalian Family of Katanin Microtubule-Severing Enzymes

“…Additionally, in non-small cell lung cancer (NSCLC), the elevated expression of KATNA1 and KATNB1 correlates with lymph node metastasis and advanced tumor progression ( Table 1 ; Wang et al, 2020 ; Ye et al, 2020 ). Katanin expression is also dysregulated in other types of cancers like papillary thyroid carcinoma (PTC), where both KATNA1 and KATNB1 are highly expressed in tumors and tumor-adjacent tissues; furthermore, elevated katanin expression correlates with advanced PTC staging and worse disease-free survival in patients ( Chen et al, 2020 ). The overexpression of katanin subunits in various cancers and findings that activating mutations in katanin contribute to cancer pathogenesis indicate that katanin dysregulation is an important step in tumorigenesis.…”