The main purpose of this study is to determine the relationship between cultural attitude, organizational commitment and motivation among academics from Turkish Republic of Northern Cyprus (TRNC) and Turkish Republic (TR) citizens. The other main aim of the study is to determine the mediating role of the cultural attitudes on the relationship of the organisational commitment and motivation. The TR and TRNC academicians of the American University of Cyprus in the Nicosia region are taken to the study. The questionnaire form, which uses a 5-point Likert scale, consists of demographic questions, organizational commitment scale, motivation scale and culture attitude scale. In this context, a total of 150 questionnaires sent to the relevant institution were distributed. Correlation analysis was conducted to examine the relationship between organizational commitment, motivation and culture attitude. PROCESS (model 4, Hayes, 2018) was used for mediation analysis. As the result of the study, the relationship between organizational commitment and motivation are found to be positive correlation among TRNC nationals but this was seen as negative correlation among TR citizens (p<0.01). For the organizational commitment positive correlations are found among the TRNC Citizens who has integration and assimilation attitudes and also TR citizens who has marginalization and separation attitudes p<0.01). For the motivation positive correlations are found among the TRNC citizens who has integration and assimilation and with the same result motivation are found to be positively corelated the TR citizens who has integration and assimilation attitude p<0.01).