Abstract. We study conical square function estimates for Banach-valued functions, and introduce a vector-valued analogue of the Coifman-Meyer-Stein tent spaces. Following recent work of Auscher-M c Intosh-Russ, the tent spaces in turn are used to construct a scale of vector-valued Hardy spaces associated with a given bisectorial operator A with certain off-diagonal bounds, such that A always has a bounded H ∞ -functional calculus on these spaces. This provides a new way of proving functional calculus of A on the Bochner spaces L p (R n ; X) by checking appropriate conical square function estimates, and also a conical analogue of Bourgain's extension of the Littlewood-Paley theory to the UMDvalued context. Even when X = C, our approach gives refined p-dependent versions of known results.