kDNA minicircle signatures of Leishmania (Viannia) braziliensis in oral and nasal mucosa from mucosal leishmaniasis patients

Oliveira, Fernanda Santos de; Valete‐Rosalino, Cláudia Maria; Schubach, Armando de Oliveira; Pacheco, Raquel da Silva

doi:10.1016/j.diagmicrobio.2009.11.004

Cited by 13 publications

(20 citation statements)

References 23 publications

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“…( V .) braziliensis subpopulations in patients with mucocutaneous leishmaniasis [20,21]. These studies served as a basis for the present investigation, which aimed to explore the relationship between genetic diversity of L .…”

Section: Discussionmentioning

confidence: 99%

“…Extraction of DNA and molecular diagnostic PCR-based assays were performed as previously described [21,22]. The 750 bp fragments generated by specific PCR were purified with a Wizard PCR Prep System (Promega, Madison, WI) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Reaction conditions and visualization of the products were performed as described by Oliveira et al . [21]. …”

Section: Methodsmentioning

confidence: 99%

“…( V .) braziliensis in ATL patients [20,21]. The purpose of the present study is to further investigate parasite subpopulations, with a particular focus on the genetic variability of L .…”

Section: Introductionmentioning

confidence: 99%

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American tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis: assessment of parasite genetic variability at intra- and inter-patient levels

et al. 2013

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BackgroundThe genetic variability of Leishmania (Viannia) braziliensis was assessed at intra and interpatient levels of individuals with different clinical manifestations of American tegumentary leishmaniasis (ATL).MethodsFifty-two samples, of which 13 originated from cutaneous lesions and 39 from mucosal lesions, provided by 35 patients, were examined by low-stringency single-specific-primer PCR (LSSP-PCR) and phenetic analysis. Genetic variability of L. (V.) braziliensis, in kinetoplast DNA (kDNA) signatures, was compared both from different patients and from different lesions of the same patient. Phenetic analysis was performed to evaluate the degree of heterogeneity of the kDNA minicircles. In order to evaluate inter and intrapatient L. (V.) braziliensis genetic variability, the percentage of shared bands and analysis of the coefficients of similarity were analyzed.ResultsDifferent genetic profiles, representing kDNA signatures of the parasite, were obtained by LSSP-PCR analysis of each sample. Phenetic analysis grouped genetic profiles of different levels of differentiation from more similar to most divergent. The percentage of shared bands at the inter and intrapatient levels was 77% and 89%, respectively. Comparison of the average inter and intrapatient coefficients of similarity and their standard deviations were statistically significant (p < 0.001).ConclusionGenetic variability at the intrapatient level was less pronounced than that between different patients. A conceptual model was proposed to better understand the complexity at both levels.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Reaction conditions and visualization of the products were performed as described by Oliveira et al . [21]. …”

Section: Methodsmentioning

confidence: 99%

“…( V .) braziliensis in ATL patients [20,21]. The purpose of the present study is to further investigate parasite subpopulations, with a particular focus on the genetic variability of L .…”

Section: Introductionmentioning

confidence: 99%

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American tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis: assessment of parasite genetic variability at intra- and inter-patient levels

et al. 2013

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“…However, despite of the effectiveness of LSSP-PCR to detect genetic variability in kinetoplastid parasites, only few studies have used LSSP-PCR for analyzing intraspecies polymorphisms in Leishmania [35]–[37].…”

Section: Introductionmentioning

confidence: 99%

KDNA Genetic Signatures Obtained by LSSP-PCR Analysis of Leishmania (Leishmania) infantum Isolated from the New and the Old World

et al. 2012

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BackgroundVisceral Leishmaniasis (VL) caused by species from the Leishmania donovani complex is the most severe form of the disease, lethal if untreated. VL caused by Leishmania infantum is a zoonosis with an increasing number of human cases and millions of dogs infected in the Old and the New World. In this study, L. infantum (syn. L.chagasi) strains were isolated from human and canine VL cases. The strains were obtained from endemic areas from Brazil and Portugal and their genetic polymorphism was ascertained using the LSSP-PCR (Low-Stringency Single Specific Primer PCR) technique for analyzing the kinetoplastid DNA (kDNA) minicircles hypervariable region.Principal FindingsKDNA genetic signatures obtained by minicircle LSSP-PCR analysis of forty L. infantum strains allowed the grouping of strains in several clades. Furthermore, LSSP-PCR profiles of L. infantum subpopulations were closely related to the host origin (human or canine). To our knowledge this is the first study which used this technique to compare genetic polymorphisms among strains of L. infantum originated from both the Old and the New World.ConclusionsLSSP-PCR profiles obtained by analysis of L. infantum kDNA hypervariable region of parasites isolated from human cases and infected dogs from Brazil and Portugal exhibited a genetic correlation among isolates originated from the same reservoir, human or canine. However, no association has been detected among the kDNA signatures and the geographical origin of L. infantum strains.

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Mucocutaneous leishmaniasis: accuracy and molecular validation of noninvasive procedures in a L. (V.) braziliensis–endemic area

Gomes¹,

Paula²,

Cesetti³

et al. 2014

Diagnostic Microbiology and Infectious Disease

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kDNA minicircle signatures of Leishmania (Viannia) braziliensis in oral and nasal mucosa from mucosal leishmaniasis patients

Cited by 13 publications

References 23 publications

American tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis: assessment of parasite genetic variability at intra- and inter-patient levels

American tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis: assessment of parasite genetic variability at intra- and inter-patient levels

KDNA Genetic Signatures Obtained by LSSP-PCR Analysis of Leishmania (Leishmania) infantum Isolated from the New and the Old World

Mucocutaneous leishmaniasis: accuracy and molecular validation of noninvasive procedures in a L. (V.) braziliensis–endemic area

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