Kedilerde MI ve MII Oositleri Kullanilarak Somatik Klonlama

Evecen, Mithat; Pabucçuoğlu, Serhat; Demır, Kadir; Yağcioğlu, Selin; Can, Ayşe; Ertürk, Ezgi; Sandal, Asiye İ̇zem; Arıcı, Ramazan; Öztürk, Gül Bakirer; Ak, Kemal; Birler, Sema

doi:10.9775/kvfd.2016.15665

Cited by 3 publications

(5 citation statements)

References 34 publications

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“…It is revealed that in vitro fertilized cat oocytes were more successful than the control group, in terms of cleavage and further embryo development in vitro (Yoshhida et al, 2022). The temperature of the transport solution was preferred by some researchers as warm (22-38 o C) (Karja et al, 2002;Merlo et al, 2005;Güriş and Birler, 2011;Evecen et al, 2016), and by some researchers as cold (5 o C) (Johnston et al, 1989;Wolfe and Wildt, 1996;Evecen et al, 2002). The ability of oocytes obtained after storing cat ovaries in cold environments for a long time to mature and be fertilized in vitro provides significant contributions to the protection of wildlife and endangered species (Wolfe and Wildt, 1996;Evecen et al, 2010;Arıcı et al, 2022).…”

Section: In Vitro Technologies a Collection And Transport Of Ovariesmentioning

confidence: 99%

“…In Vitro Maturation Media: Because there is no medium specifically designed for the in vitro maturation of cat oocytes, researchers use media that is used for other mammalian oocytes. The most commonly used media are; TCM 199 (Karja et al, 2002;Evecen et al, 2016;Evecen et al, 2002;Evecen et al, 2003a), Ham's F-10 (Evecen et al, 2016Evecen et al, 2003a). Synthetic Oviduct Fluid (Evecen et al, 2010;Evecen et al, 2009;Evecen et al, 2004), and Eagle's Minimal Essential Medium (Wolfe and Wildt, 1996).…”

Section: In Vitro Maturationmentioning

confidence: 99%

“…Most researchers add FSH and LH to the maturation medium at 1-10 µg/ml (Evecen et al, 2009;Evecen et al, 2016;Wolfe and Wildt, 1996;Evecen et al, 2002;Pope et al, 2006) and estradiol-17b at 1 µg/ ml (Wolfe and Wildt, 1996;Uchikura et al, 2011). Protein Sources: Proteins are crucial molecules for the integrity of the zona pellucida, survival, and development of oocytes/embryos in vitro.…”

Section: Supplements To In Vitro Maturation Mediamentioning

confidence: 99%

“…However, fetal cells were generally found to be better than those obtained from adults. In our previous study, where we used cumulus cells as a source of somatic cells, we produced in vitro cat embryos with SCNT (Evecen et al, 2016). Howeveralthough cytoplasts were cleaved (15.75 %) and some of them (9.58%) reached the morula cell stage, none of them could reach the blastocyst stage.…”

Section: Somatic Cell Nuclear Transfer (Cloning)mentioning

confidence: 99%

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Recent advances in assisted reproductive technologies of feline reproduction

Öndeş Candan,

Evecen

2024

J Ist Vet Sci

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Many wildcat species are threatened with extinction, rare or vulnerable due to habitat destruction and poaching. In addition, Ankara and Van domestic cat species originating from Türkiye are in danger of extinction and are under protection. Thus, the requirement for assisted reproductive techniques in b oth domestic and nondomestic cat species has been increasing in recent years. Assisted Reproductive Technologies (ART) such as in vitro maturation, in vitro fertilization, embryo transfer, and cloning in domestic cats (Felis catus) provide a useful and suitable model for the conservation of endangered cat species. Domestic cats can be recipients for embryo transfer and recipient cytoplasm for nuclear transfer from various small wildcat species. Thanks to ART, it is possible to ensure the continuation of the generation by producing in vitro embryos or by making intra or inter-species clones from wild cats that have lost their reproductive functions or even died recently. Many inherited genetic disorders have been identified in cats that are similar to humans. Due to their genetic closeness, they have recently begun to be used as animal models in some therapeutic studies on humans, especially on kidney and nervous system diseases. In the early years, in vitro study results were less successful than in farm animals but in recent years ART’s such as in vitro embryo production, embryo transfer, cloning, and transgenesis have made significant progress in domestic of domestic and wild cats. This review includes the assisted reproductive technologies applied in recent years and the results obtained in domestic cat and felines.

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Section: In Vitro Technologies a Collection And Transport Of Ovariesmentioning

confidence: 99%

Section: In Vitro Maturationmentioning

confidence: 99%

Section: Supplements To In Vitro Maturation Mediamentioning

confidence: 99%

Section: Somatic Cell Nuclear Transfer (Cloning)mentioning

confidence: 99%

See 2 more Smart Citations

Recent advances in assisted reproductive technologies of feline reproduction

Öndeş Candan,

Evecen

2024

J Ist Vet Sci

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“…In vitro maturation was performed as described in our previous study [15] . In brief, the IVM medium was modified Synthetic Oviduct Fluid, supplemented with 10 µg/mL follicle stimulating hormone (FSH), 10 µg/mL luteinizing hormone (LH), 4% bovine serum albumin (BSA, Fraction V) and antibiotics.…”

Section: In Vitro Maturation (Ivm)mentioning

confidence: 99%

Kedi Oositlerinin In Vitro Olgunlaştırılması ve Kumulus Hücrelerinin Apoptoz Oranları Üzerine, Ovaryum Taşıma ve Saklama Sıcaklığının Etkisi

Evecen¹,

Demır²,

Arıcı³

et al. 2018

Kafkas Univ Vet Fak Derg

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The objective of the present study was to examine the effects of two different transport temperature (37°C vs 4°C) and cold storage of ovaries for 24 h on cumulus cell apoptosis and maturation rates of cat oocytes in vitro. Ovaries were collected from 15 ovariohysterectomized domestic cats and maintained and transported to the laboratory in phosphate buffer saline at 37°C and 4°C. In order to determine the effects of storing time, some ovaries transported at 4°C were stored at the same temperature for 24 h. Selected cumulus oocyte complexes (COCs) were matured for 48 h at 38°C in four-well petri dishes containing 500 μL of modified oviduct medium (mSOF) under mineral oil in a 5% CO2 incubator with nearly 100% humidified. The morphological features of apoptosis were analysed in the cumulus cells at the beginning of in vitro maturation in both transporting temperature groups and after 24 h of cold stored group. The degree of apoptosis in cumulus cells were measured by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL). The IVM rates of oocytes were determined using Hoechst (33342) staining. Although the apoptotic morphological features were seen rarely and in similar rates in 37 and 4°C transporting groups (19.40 and 21.55%, P>0.001), it was seen more intensely in the 24 h cold stored group (34.80%, P<0.001). The IVM findings were similar (49.77, 44.55%) at 37°C and 4°C groups (P>0.05), and importantly lower at 4°C transporting and 24 h cold stored groups (18.90%, P<0.05). In conclusion, the results of this study suggest that (I) cumulus cells of cat oocytes are partially exposed to apoptosis during transportation at warm or cold temperature, (II) storing of ovaries for 24 h at 4°C causes apoptosis of the cumulus cells at much higher rates and (III) storing of ovaries for 24 h at 4°C affects negatively IVM rate of oocytes. Keywords: Cat, Ovary, Transport temperature, Oocyte, Cumulus, Apoptosis Kedi Oositlerinin In Vitro Olgunlaştırılması ve Kumulus Hücrelerinin Apoptoz Oranları Üzerine, Ovaryum Taşıma ve Saklama Sıcaklığının Etkisi ÖzÇalışmanın amacı, ovaryumların iki farklı sıcaklıkta (37°C ve 4°C) taşınma ve soğukta 24 saat bekletilmenin kumulus hücrelerindeki apoptoza ve kedi oositlerinin in vitro olgunlaşma oranları (IVM) üzerine etkilerini incelemektir. Kısırlaştırılmış 15 kediden ovaryumlar alındı ve yarısı 37°C, diğer yarısı da 4°C'de olmak üzere, fosfat tampon tuzlu solüsyonunda (PBS) laboratuara taşındı. Soğukta bekletmenin etkilerini belirlemek içinse, 4°C 'de taşınan ovaryumların yarısı, aynı sıcaklıkta olmak üzere 24 saat bekletildi. Seçilen kumulus oosit kompleksleri (COCs), %100'e yakın nemin sağlandığı %5 CO2'li inkübatörde mineral yağ altındaki 500 μL modifiye Sentetik Ovidukt Medyumu (mSOF) içeren dört gözlü petrilerde olmak üzere 38°C'de 48 saat süreyle olgunlaştırıldı. Apoptozun etkileri, hem iki farklı taşıma grubunda, hem de soğukta 24 saat bekletilen grupta olmak üzere in vitro olgunlaşmanın hemen öncesinde kumulus hücrelerinde test edildi. Apoptosis derecesi...

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In vitro maturation of domestic cat oocytes subjected to different incubation times

Fernandes

Santos

Macêdo

et al. 2021

RSD

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The aim of this study was to evaluate the effect of three different incubation times on in vitro maturation of domestic cat oocytes. Thus, ovaries (n = 42) were submitted to slicing procedure and the oocytes recovered were classified; only good quality oocytes (Grade I and II) underwent in vitro maturation for three different periods (24 vs. 30 vs. 36 h) in supplemented TCM-99 medium. After, oocytes were evaluated for cumulus cell expansion and presence of the first polar body. After six replicates (7 ± 1,7 ovaries per replicate), a total of 334 viable oocytes were recovered. Differences (p <0.05) were observed regarding the percentage of oocytes presenting expansion of the cumulus cells, where higher values were observed in the group of oocytes incubated for 36 h (84.3%), when compared to 30 (73.4%) and 24 h (71.0%). Moreover, differences were also observed regarding the presence of the first polar body (24 h: 29.7%; 30 h: 58.2%; 36 h: 69.8%). We conclude that the incubation period influenced the maturation rates, indicating 36 h as the ideal period for the in vitro maturation of domestic cat oocytes in supplemented TCM-199 medium.

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Kedilerde MI ve MII Oositleri Kullanilarak Somatik Klonlama

Cited by 3 publications

References 34 publications

Recent advances in assisted reproductive technologies of feline reproduction

Recent advances in assisted reproductive technologies of feline reproduction

Kedi Oositlerinin In Vitro Olgunlaştırılması ve Kumulus Hücrelerinin Apoptoz Oranları Üzerine, Ovaryum Taşıma ve Saklama Sıcaklığının Etkisi

In vitro maturation of domestic cat oocytes subjected to different incubation times

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