KEMAMPUAN EKSTRAK SENYAWA AKTIF BAKTERI ENDOFIT DALAM MENGHAMBAT PERTUMBUHAN &lt;em&gt;Fusarium oxysporum&lt;/em&gt; f.sp. PADA KELAPA SAWIT

Candrawati, Emilia; Rupaedah, Bedah; Sumpono, Sumpono; Sundaryono, Agus

doi:10.29122/jbbi.v5i2.2769

Cited by 4 publications

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“…Fusarium are widely known as a cause of plant diseases, as observed such as banana wilt disease caused by F. oxysporum f.sp. cubense [ 42 , 43 ], tomato wilt [ 44 ], onion [ 45 ], cucumber [ 46 ], oil palm [ 47 ], water hyacinth [ 48 ]. F. solani is a pathogen with symptoms of plant: stems appearing wilted and brown rot [ 49 ]; necrosis in banana seedling weeds [ 50 ]; dragon fruit plant stems ( Hylocereus sp.…”

Section: Discussionmentioning

confidence: 99%

Endophytic microbiome of Boehmeria nivea and their antagonism against latent fungal pathogens in plants

et al. 2022

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Background Pathogenic microbes still become obstacles that can reduce the quality of plant growth, including ramie (Boehmeria nivea) plants. The study identified the microbiome and antagonistic interaction of the endophytic community from the B. nivea is necessary to improve the production of the ramie plant, especially ramie stem organs for fiber materials. Results Twenty isolates of endophytic microorganisms were obtained from the roots, stems, leaves, and flowers. They were identified using the Internal Transcribed Spacer (ITS) region of ribosomal (rDNA), and its morphotypes obtained 20 isolates, with a composition of 9 species of bacteria and 11 species of fungi. Besides that, the disease observations on ramie stems showed that four species of pathogenic fungi were identified as Fusarium solani isolate 3,248,941, Fusarium solani isolates colpat-359, Fusarium oxysporum isolate N-61–2, Clonostachys rosea strain B3042. The endophytic microorganism of ramie ability was tested to determine their potential to inhibit the growth of the pathogenic fungi based on the in-vivo antagonist test. The isolated bacteria were only able to inhibit the growth of F. solani, with the highest percentage of 54–55%. Three species of endophytic fungi, including Cladosporium tennissimum, Fusarium falciforme, and Penicillium citrinum, showed the best inhibition against the fungal pathogen Fusarium solani with the highest inhibitory presentation of 91–95%. Inhibitory interaction between the endophytic microbes and the ramie pathogens indicated the type of antibiosis, competition, and parasitism. Conclusion The results of this study succeeded in showing the potential antifungal by endophytic fungi from ramie against the pathogens of the plant itself. P. citrinum isolate MEBP0017 showed the highest inhibition against all the pathogens of the ramie.

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Section: Discussionmentioning

confidence: 99%

Endophytic microbiome of Boehmeria nivea and their antagonism against latent fungal pathogens in plants

et al. 2022

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“…Setelah berumur 21 jam (OD 0,8), sebanyak 1,2 ml bakteri diambil dan dipindahkan ke dalam medium kultur NB 120 ml dan diinkubasi kembali pada suhu ruang (±27℃) menggunakan rotary shaker dengan kecepatan 100 rpm. Optical Density (OD) bakteri pada panjang gelombang 600 nm diamati selama 45 jam dengan waktu interval 3 jam (Candrawati et al 2018).…”

Section: Indeks Pelarutan =unclassified

Peranan Pseudomonas aeruginosa ATCC 15442 terhadap Pertumbuhan Tanaman Cabai Rawit (Capsicum frutescens L.) Varietas Bonita pada Kondisi Salin

Ratnasari,

Rachmania Mubarik,

Tjahjoleksono

2023

JSDH

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Hot pepper (Capsicum frutescens L.) is one of the food crop commodities that is widely cultivated in Indonesia. Salinity stress can reduce the bioavailability of potassium and its uptake by plants, which will ultimately reduce plant growth and production. One way to reduce the effect of salinity and increase potassium uptake by plants is to use potassium-solubilizing bacteria. One of the bacteria that can solubilize potassium is Pseudomonas aeruginosa. This research aims to study the role of Pseudomonas aeruginosa ATCC 15442 on the growth of the Bonita variety of hot pepper under saline conditions. This study used a completely randomized factorial design with four concentration levels of NaCl treatment: 0, 4, 8, and 12 g/L, as well as two levels of bacteria administration: without bacteria and with bacteria. The results showed that the application of bacteria, salt concentration, and the interaction of the two had no effect (p-value> 0.05) on the growth of hot pepper plants with the observed parameters namely plant height, number of leaves, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, and chlorophyll content.

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Analysis antifungal compounds produced by endophytic bacteria from oil plant using Bioautography

Candrawati

Rupaedah²,

Sumpono

et al. 2021

J. Phys.: Conf. Ser.

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This study was undertaken to evaluate the antifungal activities of the bioactive compounds poduced by endophytic bacteria isolated from oil palm plant using TLC-Bioautography. Antifungal compounds used in this study have been extracted from potential endophytic bacteria of previous studies using ethyl acetate solvent. The antifungal compounds was tested for its activity against the pathogenic fungus Fusarium oxysporum f.sp. by using TLC-Bioautography method. The results showed that there are 3 spots on the TLC plate for samples of B11 antifungal compound with an incubation time of 54 hours and B11 with an incubation time of 24 hours. Observation of bioautographic antifungal activity test showed the presence of inhibition zones with the largest average percentage given by B11 with an incubation time of 24 hours is 11.59%. The inhibition zone provided B11 with an incubation time of 54 hours is only 11.08%.

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KEMAMPUAN EKSTRAK SENYAWA AKTIF BAKTERI ENDOFIT DALAM MENGHAMBAT PERTUMBUHAN <em>Fusarium oxysporum</em> f.sp. PADA KELAPA SAWIT

Cited by 4 publications

References 0 publications

Endophytic microbiome of Boehmeria nivea and their antagonism against latent fungal pathogens in plants

Endophytic microbiome of Boehmeria nivea and their antagonism against latent fungal pathogens in plants

Peranan Pseudomonas aeruginosa ATCC 15442 terhadap Pertumbuhan Tanaman Cabai Rawit (Capsicum frutescens L.) Varietas Bonita pada Kondisi Salin

Analysis antifungal compounds produced by endophytic bacteria from oil plant using Bioautography

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