Kepone and other selected chlorinated hydrocarbon pesticides and PCBS behavior during hydraulic dredging of the James River near Hopewell, Virginia

Abstract

Field and laboratory studies were conducted on the behavior of Kepone and fifteen other chlorinated hydrocarbon pesticides and PCBs during the dredging of the James River, Virginia near Windmill Point. Over a period of six hours, water samples were collected from an area extending three miles up and downstream from the end of the dredge discharge pipe outfall. Laboratory experiments involved mixing sediment and water samples at a 1:4 ratio for 30 minutes and separation of the aqueous phase by centrifugation (elutriate test). Data on field samples including photocopying, microfilming, and recording, or by any information storage and retrieval system, without permission in writing from the publisher. Downloaded by [University of Cambridge] at 01:38 15 June 2016 262 SALEH, LEE, AND BUTLER showed that the "release" of chlorinated organic residues, including Kepone, was mainly associated with the colloidal and particulate matter. The elutriated test results indicated a slight tendency for release of chlorinated organic residues in the soluble and colloidal fraction. In samples collected from the contaminated Bailey Creek area, higher concentrations of Kepone were detected in the soluble or colloidal fraction than found from sediments collected in the nearby James River. Data on Kepone in water and sediment suggest that the compound exhibits a behavior different from the other common chlorinated organic residues.

Microbial transformation of kepone

“…aeruginosa strain K03 was isolated on UBYE agar MICROBIAL TRANSFORMATION OF KEPONE 399 (23), containing 100,ug of Kepone per ml, inoculated with the last of the series of enrichment culture transfers of a lagoon water sample. Strain K03 was the predominant morphological colony type and exhibited resistance to >1,000 ug of Kepone per ml in liquid culture.…”

“…Methods for calculating efficiency are described in detail elsewhere (Orndorff and Colwell, submitted for publication). Concentrations of Kepone residue were determined from calibration curves prepared from peak area measurements of Kepone (23) and from peak height measurements, using the formula: residue (ppm) = V wd2/W vd,, where W is grams of sample, V is milliliters of final extract, v is microliter injected, w is nanograms of standard injected, di is peak height of standard solution, and d2…”