Keragaman Genetika Empat Belas Aksesi Kentang (Solanum tuberosum L.) Berdasarkan Marka SSR dan STS (Genetic Diversity of Fourteen Potato Accessions Based on SSR and STS Markers)

Nugroho, Kristianto; Reflinur, Reflinur; Lestari, Puji; Rosdianti, Ida; Terryana, Rerenstradika Tizar; Kusmana, Kusmana; Tasma, I Made

doi:10.21082/jbio.v11n2.2015.p41-48

Cited by 10 publications

(18 citation statements)

References 11 publications

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“…Sebelumnya, Runtunuwu et al (2011) dan Kawengian et al (2016) telah memanfaatkan marka Random Amplified Polymorphic DNA (RAPD) dalam menganalisis diversitas genetik kentang, akan tetapi materi genetik dan marka molekuler yang digunakan jumlahnya masih terbatas. Sementara itu, Nugroho et al (2015) telah menggunakan marka SSR yang berasal dari beberapa referensi untuk menganalisis diversitas genetik berbagai aksesi plasma nutfah kentang di Indonesia. Hasilnya menunjukkan bahwa aksesi SDG kentang mengelompok menjadi dua klaster utama.…”

Section: Pendahuluanunclassified

“…Sementara, ringkasan statistik analisis polimorfisme marka SSR yang diuji disajikan pada Tabel 4. Jumlah marka polimorfik yang dihasilkan pada penelitian ini jauh lebih banyak dibanding dengan hasil penelitian sebe-lumnya (Nugroho et al 2015) yang hanya memperoleh 12 marka polimorfik dari 19 marka yang digunakan. Persentase marka polimorfik yang diperoleh pada penelitian ini sebesar 91,6%, meningkat dari penelitian sebelumnya yang hanya sebesar 63,2%.…”

Section: Polimorfisme Marka Ssrunclassified

“…Kedua genotipe tersebut memiliki hubungan kekerabatan yang dekat karena genotipe GM05 merupakan hasil persilangan antara genotipe Granola dan Michigan Pink (Tabel 1). Hasil serupa diperoleh sebelumnya pada penelitian Nugroho et al (2015), kedua genotipe tersebut berada pada klaster yang sama.…”

Section: Analisis Filogenetikunclassified

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Genetic Diversity Analysis of 14 Potato Genotypes Based on Morphological Characters and SSR Markers

Nugroho¹,

Terriyana²,

Kusmana³

et al. 2019

J. AgroBiogen

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Potato is one of important carbohydrate sources used as an alternative crop in Indonesia. The challenges in national potato breeding program included low productivity, less tolerance to environmental stresses, and narrow genetic diversity. The purpose of this study was to analyze genetic diversity of 14 potato genotypes based on morphological characters and SSR markers newly developed from genome sequences of Indonesian potato genotypes. Principal component analysis of morphological data was done using program XLSTAT. DNA of 14 potato genotypes were assayed using 22 SSR markers. Phylogenetic tree was constructed using program NTSYS version 2.1. The PCA showed that leaf shape, leaf color, tuber shape, tuber skin color, and tuber color contributed most to the total diversity. SSR polymorphism analysis demonstrated that as many as 196 alleles were detected in this study. The average allele number was 8.9 ranged from 5 to 13 alleles per locus. The average major allele frequencies was 22% ranged from 14 to 43%. Gene diversity ranged from 0.70 to 0.92 with the average of 0.86, meanwhile the heterozigosity observed ranged from 0 to 0.71 with the average of 0.05. Phylogenetic analysis generated two main clusters in the coefficient of similarity 0.77. The first cluster consisted of three genotypes while the second cluster consisted of eleven genotypes. The new developed SSR markers used in this study were able to differentiate potato accessions having similar morphological characters but were different genetically. The results of this study should be useful in assessing genetic materials in potato cultivar development program.

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Section: Pendahuluanunclassified

Section: Polimorfisme Marka Ssrunclassified

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Genetic Diversity Analysis of 14 Potato Genotypes Based on Morphological Characters and SSR Markers

Nugroho¹,

Terriyana²,

Kusmana³

et al. 2019

J. AgroBiogen

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“…Indonesia dikenal sebagai salah satu negara yang memiliki keragaman genetik padi Javanika (padi bulu) yang cukup tinggi. Nugroho et al, (2015), melaporkan bahwa saat ini di Indonesia, kurang lebih 4000 varietas padi yang tergolong berbagai subspesies termasuk varietas unggul maupun lokal tersedia di bank gen Balai Besar Bioteknologi dan Sumber Daya Genetik Pertanian.…”

Section: Plasma Nutfah Padiunclassified

“…Data yang terkumpul akan dianalisis dengan menggunakan analisis kekerabatan. Analisis kekerabatan dilakukan melalui analisis cluster (gerombol) dengan menggunakan metode Sequential Agglomerative Hierarchiel and Nasted (SAHN)-UPGMA (Unweighted Pair Group Method With Arithmatic) menggunakan software NTSYS-pc versi (Numerical Taxonomy and Multivarieted Analisys System) versi 2,02 (Rohlf 2000 dalam Nugroho et al, 2015). Analisis cluster akan mengklasifikasikan objek sehingga setiap objek yang paling dekat kesamaannya dengan objek lain berada dalam cluster yang sama (Ediyanto et al, 2013).…”

Section: Metodeunclassified

Karakterisasi Morfologi Malai Plasma Nutfah Padi Lokal Asal Kabupaten Rokan Hilir, Riau

2019

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Abstrak. Introduksi varietas padi unggul dan konversi lahan sawah yang terjadi diberbagai daerah di Indonesia menyebabkan tergerusnya keragaman genetik padi lokal. Peningkatan dan pelestarian genetik padi dapat dilakukan melalui kegiatan eksplorasi dan karakterisasi. Kabupaten Rokan Hilir merupakan salah sentra produksi padi di Provinsi Riau dan diindikasikan memiliki banyak plasma nutfah padi lokal. Penelitian ini bertujuan untuk memperoleh data keragaan dan hubungan kekerabatan plasma nutfah padi lokal asal Kabupaten Rokan Hilir berdasarkan karakter morfologi malai. Metode penelitian yang digunakan yaitu metode kualitatif bersifat deskriptif analisis, meliputi karakterisasi dan analisis kekerabatan. Hasil analisis kekerabatan terhadap karakter morfologi 16 plasma nutfah padi lokal asal Kabupaten Rokan Hilir terbagi atas 6 kelompok pada koefisien kemiripan 0,45 (45 %). Kelompok I, II, dan IV masing-masing terdiri atas empat aksesi. Kelompok III dan VI masing-masing terdiri atas satu aksesi. Kelompok V terdiri atas dua aksesi. Plasma nutfah padi lokal Kabupaten Rokan Hilir menunjukan keragaan karakter morfologi malai yang cukup beragam.

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Selection of potato molecular markers of Granola L. variety

Sa’adah

Hartanto²,

Sahat³

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Granola L. is one of potato variety that is very popular in Indonesia and is currently difficult to replace by other varieties. One of the efforts to maintain the genetic purity of the Granola L. variety can be done through biotechnology technique using molecular markers. This research aimed to obtain molecular markers for the Granola L variety. The selection of molecular markers was carried out at the Molecular Biology Laboratory and screenhouse of Indonesian Vegetable Research Institute (IVEGRI) from March to October 2020. The treatments consisted of DNA of Granola L variety from Source Seed Management Unit (UPBS) - IVEGRI, 8 DNA genotypes/germplasm collections which were assumed to be Granola or has a Granola background (Granola BPBK, GM-05, G2, G8, G-771, G-772, GC21, Papita), and 2 DNA genotypes that do not have Granola background (non-Granola) namely Spudi and Amabil varieties. The molecular markers used in this study were 17 SSR primers (Simple Sequence Repeats) from Indonesian Agricultural Genome Center. The results showed that 11 primers were monomorphic and 6 primers were polymorphic. These polymorphic primers still cannot specifically distinguish between Granola L. and non-Granola L. varieties. There is one primer that can distinguish Granola and non-Granola in terms of the band size formed. Further research is needed to find molecular markers that can distinguish Granola L from varieties/genotypes/germplasm collections that are assumed to be Granola L. or having the Granola L. genetic background.

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Keragaman Genetika Empat Belas Aksesi Kentang (Solanum tuberosum L.) Berdasarkan Marka SSR dan STS (Genetic Diversity of Fourteen Potato Accessions Based on SSR and STS Markers)

Cited by 10 publications

References 11 publications

Genetic Diversity Analysis of 14 Potato Genotypes Based on Morphological Characters and SSR Markers

Genetic Diversity Analysis of 14 Potato Genotypes Based on Morphological Characters and SSR Markers

Karakterisasi Morfologi Malai Plasma Nutfah Padi Lokal Asal Kabupaten Rokan Hilir, Riau

Selection of potato molecular markers of Granola L. variety

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