Keratin 19 marks poor differentiation and a more aggressive behaviour in canine and human hepatocellular tumours

Sprundel, Renee G H M van; Ingh, T.S.G.A.M. van den; Desmet, Valeer; Katoonizadeh, Aezam; Penning, Louis C.; Rothuizen, J.; Roskams, Tania; Spee, Bart

doi:10.1186/1476-5926-9-4

Cited by 37 publications

(39 citation statements)

References 41 publications

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“…Therefore, this could be a possible reason why K19-positive HCC shows aggressive behavior in comparison with K19-negative HCC. In fact, previous publications and our study confirm these features [27]. …”

Section: Discussionsupporting

confidence: 81%

Expression of Keratin 19 Is Related to High Recurrence of Hepatocellular Carcinoma after Radiofrequency Ablation

et al. 2011

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Objective: Keratin (K) 19 positivity has been reported to be a useful predictive marker for recurrence in patients with hepatocellular carcinoma (HCC) who have undergone hepatic resection. We investigated the clinical usefulness of K19 positivity in patients who had received curative radiofrequency ablation (RFA). Methods: We retrospectively evaluated the clinicopathological features, including imaging and K19 expression, in 246 patients with HCC who were within the Milan criteria and had received curative RFA. Using a two-step insertion method, tumor biopsies were obtained just prior to RFA and were evaluated histologically. Results: Tumor seeding due to liver biopsy and RFA was not observed. Ten patients (4.1%) had K19-positive HCC. Imaging findings were similar between K19-positive and -negative HCC (p = 0.187). Nine out of 10 patients (90%) who had K19-positive HCC had recurrence of HCC after RFA, and intrahepatic recurrences were observed within 12 months in 6 out of 10 (60.0%). K19 positivity was a significant risk factor for recurrence (p < 0.0001) and early recurrence (<1 year after RFA; p =0.012). K19 expression (p = 0.016) was an independent risk factor for tumor status exceeding the Milan criteria after RFA. Conclusion: Expression of K19 is related to high recurrence of HCC after curative RFA.

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Section: Discussionsupporting

confidence: 81%

Expression of Keratin 19 Is Related to High Recurrence of Hepatocellular Carcinoma after Radiofrequency Ablation

et al. 2011

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“…In the case of chymotrypsin C suppression, cytokeratin 18, keratin 19, and prolyl 4-hydroxylase were upregulated 1.31, 1.28, and 1.55 folds, respectively, whereas lamin A/C and heat shock 70 kDa protein 8 were downregulated to about 60 or 79%, respectively. All of these differentially expressed proteins have been reported to be involved in cancer metastasis [12][13][14][15][16][17][18][19][20][21][22][23]. Although the above proteins are all associated with cancer, they (with the exception of cytokeratin 18) had no close correlation with either upregulation or suppression of chymotrypsin C. The changes at protein levels of cytokeratin 18 were also consistent with its mRNA level, which was verified by quantitative RT-PCR.…”

Section: Chymotrypsin C and Related Proteins Involved In The Aspc-1 Csupporting

confidence: 58%

Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration

Wang¹,

Sha²,

Liu³

et al. 2011

ABBS

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Pancreatic cancer is a malignant cancer with a high mortality rate. The amount of chymotrypsin C in pancreatic cancer cells is only 20% of that found in normal cells. Chymotrypsin C has been reported to be involved in cancer cell apoptosis, but its effect on pancreatic cancer cell migration is unclear. We performed cell migration scratch assays and Transwell experiments, and found that cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed chymotrypsin C, whereas the cell migration ability was upregulated in Aspc-1 cells in which chymotrypsin C was suppressed. Two-dimensional fluorescence differential in gel electrophoresis/mass spectrometry method was used to identify the proteins that were differentially expressed in Aspc-1 cells that were transfected with plasmids to induce either overexpression or suppressed expression of chymotrypsin C. Among 26 identified differential proteins, cytokeratin 18 was most obviously correlated with chymotrypsin C expression. Cytokeratin 18 is expressed in developmental tissues in early stages of cancer, and is highly expressed in most carcinomas. We speculated that chymotrypsin C might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression.

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“…CK19 expression has been shown to be a poor prognostic factor in patients with HCC. Previous study demonstrated that HCC expressing CK19 expression was associated with early postoperative recurrence [30]. Furthermore, the higher recurrence rate of CK19-positive HCC after transplantation suggests a worse prognosis for HCC expressing CK19 compared to CK19-negative HCC [31].…”

Section: Discussionmentioning

confidence: 96%

Prognostic implications of tumor vascularity and its relationship to cytokeratin 19 expression in patients with hepatocellular carcinoma

et al. 2011

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Keratin 19 marks poor differentiation and a more aggressive behaviour in canine and human hepatocellular tumours

Cited by 37 publications

References 41 publications

Expression of Keratin 19 Is Related to High Recurrence of Hepatocellular Carcinoma after Radiofrequency Ablation

Expression of Keratin 19 Is Related to High Recurrence of Hepatocellular Carcinoma after Radiofrequency Ablation

Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration

Prognostic implications of tumor vascularity and its relationship to cytokeratin 19 expression in patients with hepatocellular carcinoma

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