Keratin and vimentin distribution patterns in the epithelial structures of the cannie anal region

Abstract

The intermediate filament labeling pattern of the epithelial structures of the canine anal region was studied with different polypeptide specific keratin monoclonal antibodies (MoAbs) and with a monoclonal and polyclonal vimentin antibody. The epithelial structures in this region could be discriminated and characterized by differences in their keratin staining pattern. The basal cells in the different epithelial structures showed a similar staining pattern characterized by reactivity with MoAbs staining keratins 5, 8, 14, and 17. Columnar epithelial cells showed a completely different phenotype mostly characterized by reactivity with MoAbs staining keratins 7, 5, 8, 18, and 19. A restricted number of differentiated perianal gland cells showed perinuclear vimentin staining. Myoepithelial cells did not stain for vimentin, but, as other basal cells, were positive for MoAbs staining keratins 5, 8, 14, and 17.

The Expression of Keratins, Vimentin, Neurofilament Proteins, Smooth Muscle Actin, Neuron-specific Enolase, and Synaptophysin in Tumors of the Specific Glands in the Canine Anal Region

“…In human beings, keratins 7 and 18 are considered typical of glandular differentiati~n.~~ MoAbs directed against these keratins (RCK 105 and RGE 53/DE-K 18, respectively) stained in the canine anal region: in the luminal cells of the anal glands, anal sac glands, and sweat glands. 78 The staining of the carcinoma cells with these antibodies, therefore, is consistent with neoplastic transformation of luminal cells of tubular glands. This interpretation is supported by the absence of tumor cell labeling by the a-smooth muscle actin antibody, which exclusively stained basally localized (probably myoepithelial) cells in the three glandular structures.…”

“…The presence of scattered solitary vimentin-positive tumor cells in the perianal gland tumors is in accordance with the staining of some differentiated cells in normal perianal glands. 78 In conclusion, epithelial tumors specific for the anal region in the dog can be differentiated immunohistochemically into perianal gland tumors and non-perianal gland carcinomas, based on their keratin staining pattern. Although a minority of tumor cells in the carcinomas appeared to be NSE positive, unequivocal and complete NE differentiation of these tumors was not evident.…”

“…The results of tumor immunohistochemical evaluation with respect to the various keratins and vimentin were compared with data obtained from a previous study on the immunohistochemical characteristics of the epithelial structures of the canine anal region. 78 The immunohistochemical findings of the specific glandular structures in this region are shown in Table 2. The results with respect to NSE, synaptophysin, Immunohistochemical findings* for various anti-human keratins and anti-calf vimentin in the specific glandular and a-smooth muscle actin were compared with the findings in structures of normal dogs as described in this study.…”

Circumanal glands of the dog: A new classification and cell degeneration

“…In particular, the predominant distribution of bundles of tonofilaments connected to cytoplasmic regions of desmosomes in polyhedral cells is in good agreement with the intense ring-like immunostaining of keratin. Polyhe- dral cells were shown to contain keratin-specific epitopes in a previous study (Vos et al, 1992) and in the present study. Even with the electron microscope, no secretory granules are found.…”

Cellular terrain surrounding sympathetic nerve pathways in the rat orbit: Comparisons of orbital connective tissue and smooth muscle cell phenotypes

“…They contained high levels of ␣-SMA-ir, a contractile protein indicative of relatively few cell types, including myoepithelial cells (Destexhe et al, 1993), smooth muscle cells (Gabbiani et al, 1981;Skalli et al, 1986), and myofibroblasts (Skalli et al, 1989). The possibility that the pathway is composed of myoepithelial cells can be excluded, however, by the absence of cytokeratin coupled with high vimentin immunoreactivity, features that are incompatible with the myoepithelial cell phenotype (Vos et al, 1992;Destexhe et al, 1993). Although pathway cells have many characteristics in common with smooth muscle, they also show important differences.…”