Keratin immunohistochemistry in normal human liver. Cytokeratin pattern of hepatocytes, bile ducts and acinar gradient

Eyken, Peter Van; Sciot, Raphael; Damme, B Van; Wolf‐Peeters, Chris De; Desmet, V J

doi:10.1007/bf00750732

Cited by 142 publications

(62 citation statements)

References 21 publications

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“…Although oval cells are thought to emanate from the periportal area [4][5][6][7][8], their lineage remains controversial. Immunofluorescence studies revealed that oval cells express cytokeratin 19 (CK19), a marker specific for the biliary tree that includes bile ducts as well as the canal of Hering [8,9]. OV6, the most widely used monoclonal antibody to identify oval cells in rat liver [10], stains similar structures [11], indicating that epithelial cells residing within the canal of Hering are the most probable candidates for resident liver stem cells [12].…”

Section: Introductionmentioning

confidence: 99%

Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins

Corcelle¹,

Stieger

Gjinovci³

et al. 2006

Experimental Cell Research

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Despite extensive studies, the hematopoietic versus hepatic origin of liver progenitor oval cells remains controversial. The aim of this study was to determine the origin of such cells after liver injury and to establish an oval cell line. Rat liver injury was induced by subcutaneous insertion of 2-AAF pellets for 7 days with subsequent injection of CCl 4 . Livers were removed 9 to 13 days post-CCl 4 treatment. Immunohistochemistry was performed using anti-c-kit, OV6, Thy1, CK19, AFP, vWF and Rab3b. Isolated non-parenchymal cells were grown on mouse embryonic fibroblast, and their gene expression profile was characterized by RT-PCR. We identified a subpopulation of OV6/CK19/Rab3b-expressing cells that was activated in the periportal region of traumatized livers. We also characterized a second subpopulation that expressed the HSCs marker c-kit but not Thy1. Although we successfully isolated both cell types, OV6/CK19/Rab3b + cells fail to propagate while c-kit + -HSCs appeared to proliferate for up to 7 weeks. Cells formed clusters which expressed c-kit, Thy1 and albumin. Our results indicate that a bona fide oval progenitor cell population resides within the liver and is distinct from c-kit + -HSCs. Oval cells require the hepatic niche to proliferate, while cells mobilized from the circulation proliferate and transdifferentiate into hepatocytes without evidence of cell fusion.

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Section: Introductionmentioning

confidence: 99%

Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins

Corcelle¹,

Stieger

Gjinovci³

et al. 2006

Experimental Cell Research

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“…We have compared the pattern of OV-6 expression with two known biliary cell markers, human epithelial antigen-125 (HEA-125) and the intermediate filament cytokeratin . [20][21][22][23] …”

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confidence: 99%

Immunolocalization of OV-6, a putative progenitor cell marker in human fetal and diseased pediatric liver

et al. 1998

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The existence of progenitor (stem) cells in the human liver remains a matter of debate. In rodent models of hepatocarcinogenesis and injury, oval cells proliferate in the periportal regions of the portal tracts and are suggested to derive from a stem cell compartment, because they are capable of differentiating into hepatocytes or biliary epithelial cells. In this study, the rat oval cell marker, OV-6 has been used to investigate the hypothesis that there are stem cells present in fetal and pediatric human liver. The pattern of OV-6 expression was compared with the established adult biliary cell markers human epithelial antigen-125 (HEA-125) and cytokeratin-19 (CK-19). In normal pediatric liver (n ‫؍‬ 7), bile ducts and ductules were immunostained with CK-19 and HEA-125, whereas OV-6 staining was consistently negative. In fetal tissue (n ‫؍‬ 10), ductal plate cells, primitive bile ducts, and hepatoblasts were stained with CK-19 and HEA-125 although only some of the ductal plate cells and hepatoblasts were OV-6 positive. In biliary atresia (n ‫؍‬ 6) and ␣1, anti-trypsin deficiency (␣1,AT) (n ‫؍‬ 4), CK-19 and HEA-125 immunostained ductular proliferative cells that tended to form finely anastomosing ductules, whereas OV-6 staining was found more on discrete cells confined to portal tract margins. Additionally, in diseased liver, OV-6 was strongly positive in hepatocyte lobules with greatest intensity in the periseptal regions. This widespread hepatocyte OV-6 positivity suggests that the antibody may identify cells of a less differentiated phenotype (transitional hepatocytes) that have replaced the mature cells. Therefore, it is proposed that in human liver, OV-6 is recognizing cells with a progenitor stem cell-like phenotype with the capacity to differentiate into OV-6 positive ductular cells or lobular hepatocytes. (HEPATOLOGY 1998;28:980-985.)Human liver organogenesis begins during the third or fourth week of gestation from endodermal cells lining the foregut. These endodermal cells give rise to hepatic cords representing liver progenitor cells (hepatoblasts), which are able to differentiate along biliary or hepatocyte lineages. 1

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“…Inc., (San Mateo, CA, USA). Monoclonal antibodies (KL-1 and CAM) against cytokeratin are known to stain hepatocytes as well as biliary epithelial cells in man (Van Eyken et al 1987).…”

Section: Methodsmentioning

confidence: 99%

Localization of actin and cytokeratin in hepatocytes and biliary epithelial cells in normal human livers using a low temperature resin and post-embedding immunohistochemistry.

Kono

Nakanuma

1989

Tohoku J. Exp. Med.

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Keratin immunohistochemistry in normal human liver. Cytokeratin pattern of hepatocytes, bile ducts and acinar gradient

Cited by 142 publications

References 21 publications

Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins

Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins

Immunolocalization of OV-6, a putative progenitor cell marker in human fetal and diseased pediatric liver

Localization of actin and cytokeratin in hepatocytes and biliary epithelial cells in normal human livers using a low temperature resin and post-embedding immunohistochemistry.

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