Localization of actin and cytokeratin in hepatocytes and biliary epithelial cells in normal human livers using a low temperature resin and post-embedding immunohistochemistry.

Kono, Naoko; Nakanuma, Yasuni

doi:10.1620/tjem.157.325

Tohoku J. Exp. Med.

1989

DOI: 10.1620/tjem.157.325

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Localization of actin and cytokeratin in hepatocytes and biliary epithelial cells in normal human livers using a low temperature resin and post-embedding immunohistochemistry.

Naoko Kono

Yasuni Nakanuma

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Cited by 5 publications

(1 citation statement)

References 16 publications

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“…Fresh fragments of one histologically normal liver (from a 56-yr-old woman), one liver of EBO (duration of jaundice ; = 2 mo, from a 53-yr-old woman) and two livers of PBC (stage 2, from 45-yr-old woman and 36-yr-old man), all obtained at laparotomy, were examined under immunoelectron microscopy for Lewis Y, with a postembedding method (23,24). These biopsy specimens, also included in the immunohistochemical study (see above), were dissected into small cubes and fued by 1%' glutaraldehyde.…”

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confidence: 99%

Neoexpression of Lewis Y antigen is a sensitive phenotypic change of the damaged intrahepatic bile ducts

1994

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We examined the expression of Lewis antigens, particularly Lewis Y, on the intrahepatic biliary epithelial cells in normal livers and various hepatobiliary diseases with immunohistochemical and immunoelectron microscopic methods. In normal livers, Lewis Y was consistently and generally negative in the bile ductules and small bile ducts, respectively. This antigen was frequently and strongly expressed on these ducts and ductules showing variable pathological changes such as necroinflammation and proliferation in a majority of hepatobiliary diseases independent on their etiology, whereas a majority of normal-appearing bile ducts and ductules in these pathological conditions were negative. Immunoelectron microscopically, gold particles suggesting the presence of Lewis Y were demonstrated on microvilli facing the biliary lumen, lateral cell membranes, secretory granules and Golgi apparatus of abnormal biliary epithelial cells in various hepatobiliary diseases. These data suggest that neoexpression of Lewis Y antigen is a highly sensitive, nonspecific phenotypic change of carbohydrate residues occurring in the abnormal biliary epithelial cells in various hepatobiliary diseases.

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confidence: 99%

Neoexpression of Lewis Y antigen is a sensitive phenotypic change of the damaged intrahepatic bile ducts

1994

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Ultrastructural and immunohistochemical studies of the intrahepatic peribiliary capillary plexus in normal livers and extrahepatic biliary obstruction in human beings

Kono

Nakanuma

1992

Hepatology

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Ultrastructural and immunohistochemical study was conducted on the intrahepatic peribiliary capillary plexus in normal livers and in those with extrahepatic biliary obstruction. In both conditions, capillaries positive for Ulex europaeus agglutinin I and type IV collagen were always present in the vicinity of the bile ducts. Immunoelectron microscopy showed the presence of type IV collagen on the basal lamina of these capillaries; Ulex europaeus agglutinin I was also positive on their cytoplasms. Under electron microscope, a considerable number of these capillaries were seen as being composed of fenestrated endothelium with a diaphragm and with extreme cytoplasmic attenuations that were dense at the sides facing the bile duct in comparison with the opposite sides in normal livers. In extrahepatic biliary obstruction, plasmalemmal pinocytic vesicles, multivesicular bodies and other cellular organellae such as rough endoplasmic reticulum and Weibel-Palade bodies increased in number in these capillaries, relative to normal livers, probably reflecting increased permeability and functional activities. These characteristic ultrastructural features of the peribiliary capillary plexus might be arranged to transport substances effectively by way of intrahepatic biliary epithelial cells in normal livers and also might be altered to meet the increased functional demands of extrahepatic biliary obstruction.

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Contractile proteins in podocytes: immunocytochemical localization of actin and alpha-actinin in normal and nephrotic rat kidneys

Lachapelle

Bendayan

1991

Virchows Archiv B Cell Pathol

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Localization of actin and cytokeratin in hepatocytes and biliary epithelial cells in normal human livers using a low temperature resin and post-embedding immunohistochemistry.

Cited by 5 publications

References 16 publications

Neoexpression of Lewis Y antigen is a sensitive phenotypic change of the damaged intrahepatic bile ducts

Neoexpression of Lewis Y antigen is a sensitive phenotypic change of the damaged intrahepatic bile ducts

Ultrastructural and immunohistochemical studies of the intrahepatic peribiliary capillary plexus in normal livers and extrahepatic biliary obstruction in human beings

Contractile proteins in podocytes: immunocytochemical localization of actin and alpha-actinin in normal and nephrotic rat kidneys

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