Neoexpression of Lewis Y antigen is a sensitive phenotypic change of the damaged intrahepatic bile ducts

We investigated whether carbohydrate antigens on cholangitis, 2,4 in which the proliferated peribiliary biliary glycoproteins and carcinoembryonic antigen glands secrete large amounts of acid (sialylated and (CEA) are related to hepatolithiasis. CEA, ABO, and sulfated) or neutral mucin into the biliary lumen. 4 Most Lewis blood group-related antigens, as well as sialylintrahepatic calculi consist of calcium-bilirubinate Tn antigen in hepatic bile, were analyzed by Western (brown pigment) stones. 4,5 Bacterial infection, bile stablotting in samples from 12 patients with hepatolithiasis sis, and an alteration of the bile composition are and 37 with other biliary diseases (choledocholithiasis, thought to be responsible for the nucleation, formation, 13; cholecystolithiasis, 5; acute cholecystitis, 2; choland maturation of intrahepatic calculi. 6,7 In addition, angiocarcinoma, 5; common bile duct carcinoma, 4; panthe increased mucin production and secretion from the creatic carcinoma, 6; and metastatic carcinoma of liver, bile ducts may play an important role in the develop-2). CEA was positive on mucinous glycoprotein in six patients (50%) with hepatolithiasis and one case (17%) ment of intrahepatic calculi. 4,5,8 Biliary mucin, particuwith pancreatic carcinoma. CEA was also positive on a larly sulfated, reportedly promotes the formation of calglycoprotein of approximately 200 kd in eight patients cium-bilirubinate stones. 4,7 Histochemically, these (67%) with hepatolithiasis and two (33%) with pancreatic stones are composed of alternating multilayers of mucarcinoma. Lewis X was detected on the mucinous glycocin and pigment (calcium-bilirubinate) on their cut surprotein in almost all samples, as well as on glycoproteins face, suggesting that mucin bridges the pigment layers of approximately 180 kd in all hepatolithiasis samples and participates in the precipitation of calcium-biliruand approximately half of those from patients with other binate. 9 diseases. Sialyl-Tn antigen was detected on mucinousCarbohydrate antigens of glycoproteins, glycolipids, glycoprotein in four (80%) with cholangiocarcinoma, two and proteoglycans play an important role in cell-to-

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confidence: 99%

Carcinoembryonic antigen and blood group-related carbohydrate antigens in glycoproteins in human bile in hepatolithiasi

et al. 1996

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“…For comparison, another three cell lines, Hep3B, HT-29 and COS7, were also studied. As the hepatoma-derived cell line, Hep3B cells have been reported to express high level of glycan of Le y 42. HT-29 cells, as human colon cancer cells, were chosen due to their reported high expression of the cancer-associated glycan of sLe a 4.…”

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confidence: 99%

In situ recognition of cell-surface glycans and targeted imaging of cancer cells

Cheng

Chen

et al. 2013

Sci Rep

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Fluorescent sensors capable of recognizing cancer-associated glycans, such as sialyl Lewis X (sLex) tetrasaccharide, have great potential for cancer diagnosis and therapy. Studies on water-soluble and biocompatible sensors for in situ recognition of cancer-associated glycans in live cells and targeted imaging of cancer cells are very limited at present. Here we report boronic acid-functionalized peptide-based fluorescent sensors (BPFSs) for in situ recognition and differentiation of cancer-associated glycans, as well as targeted imaging of cancer cells. By screening BPFSs with different structures, it was demonstrated that BPFS1 with a FRGDF peptide could recognize cell-surface glycan of sLex with high specificity and thereafter fluorescently label and discriminate cancer cells through the cooperation with the specific recognition between RGD and integrins. The newly developed peptide-based sensor will find great potential as a fluorescent probe for cancer diagnosis.

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“…a tumor marker, 16 also functions as an intercellular Sialyl-Tn antigen in biliary mucinous glycoprotein will adhesion molecule 17,18 and also binds and aggregates be a good marker of biliary and pancreatic carcinoma, bacteria. 19,20 Immunohistochemical studies [21][22][23] have and probably for cholangiocarcinoma complicated with disclosed that the biliary epithelial cells at different anatomic levels have a characteristic distribution of carbohydrate antigens, and their altered distribution Hepatolithiasis is quite common in the Far East, in-is associated with biliary tract diseases. It is therefore cluding Japan, although it is extremely rare in Western likely that alterations of the carbohydrate residues of countries.…”

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confidence: 99%

Carcinoembryonic antigen and blood group-related carbohydrate antigens in glycoproteins in human bile in hepatolithiasi

Sasaki

1996

Hepatology

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We investigated whether carbohydrate antigens on cholangitis, 2,4 in which the proliferated peribiliary biliary glycoproteins and carcinoembryonic antigen glands secrete large amounts of acid (sialylated and (CEA) are related to hepatolithiasis. CEA, ABO, and sulfated) or neutral mucin into the biliary lumen. 4 Most Lewis blood group-related antigens, as well as sialyl-intrahepatic calculi consist of calcium-bilirubinate Tn antigen in hepatic bile, were analyzed by Western (brown pigment) stones. 4,5 Bacterial infection, bile stablotting in samples from 12 patients with hepatolithiasis sis, and an alteration of the bile composition are and 37 with other biliary diseases (choledocholithiasis, thought to be responsible for the nucleation, formation, 13; cholecystolithiasis, 5; acute cholecystitis, 2; chol-and maturation of intrahepatic calculi. 6,7 In addition, angiocarcinoma, 5; common bile duct carcinoma, 4; panthe increased mucin production and secretion from the creatic carcinoma, 6; and metastatic carcinoma of liver, bile ducts may play an important role in the develop-2). CEA was positive on mucinous glycoprotein in six patients (50%) with hepatolithiasis and one case (17%) ment of intrahepatic calculi. 4,5,8 Biliary mucin, particuwith pancreatic carcinoma. CEA was also positive on a larly sulfated, reportedly promotes the formation of calglycoprotein of approximately 200 kd in eight patients cium-bilirubinate stones. 4,7 Histochemically, these (67%) with hepatolithiasis and two (33%) with pancreatic stones are composed of alternating multilayers of mucarcinoma. Lewis X was detected on the mucinous glyco-cin and pigment (calcium-bilirubinate) on their cut surprotein in almost all samples, as well as on glycoproteins face, suggesting that mucin bridges the pigment layers of approximately 180 kd in all hepatolithiasis samples and participates in the precipitation of calcium-biliruand approximately half of those from patients with other binate. and proteoglycans play an important role in cell-to-(50%) with common bile duct carcinoma, and all pancreatic carcinoma samples. Mucinous glycoprotein and gly-cell adhesion and recognition 10-13 and also have a close coproteins containing CEA and Lewis X antigens are en-relationship with a specific bacterial infection.14,15 Carriched in hepatic bile of hepatolithiasis, and they may be cinoembryonic antigen (CEA), which is widely used as closely related to the formation of intrahepatic calculi. a tumor marker, 16 also functions as an intercellular Sialyl-Tn antigen in biliary mucinous glycoprotein will adhesion molecule 17,18 and also binds and aggregates be a good marker of biliary and pancreatic carcinoma, bacteria. 19,20 Immunohistochemical studies 21-23 have and probably for cholangiocarcinoma complicated with disclosed that the biliary epithelial cells at different hepatolithiasis. (HEPATOLOGY 1996;23:258-263.) anatomic levels have a characteristic distribution of carbohydrate antigens, and their altered distribution Hepatolithiasis is quite common in the Fa...

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Neoexpression of Lewis Y antigen is a sensitive phenotypic change of the damaged intrahepatic bile ducts

Cited by 9 publications

References 25 publications

Carcinoembryonic antigen and blood group-related carbohydrate antigens in glycoproteins in human bile in hepatolithiasi

Carcinoembryonic antigen and blood group-related carbohydrate antigens in glycoproteins in human bile in hepatolithiasi

In situ recognition of cell-surface glycans and targeted imaging of cancer cells

Carcinoembryonic antigen and blood group-related carbohydrate antigens in glycoproteins in human bile in hepatolithiasi

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