Keratinolytic Protease Production by Bacillus amyloliquefaciens 6B Using Feather Meal as Substrate and Application of Feather Hydrolysate as Organic Nitrogen Input for Agricultural Soil

Bose, Anjali; Pathan, Shabnam; Pathak, Khyatiben V.; Keharia, Haresh

doi:10.1007/s12649-013-9272-5

Cited by 53 publications

(21 citation statements)

References 48 publications

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“…FPF-1. In a similar study, 0.5% xylose was used as the fermentation medium, and Bacillus amyloliquefaciens B6 achieved a higher concentration of keratinase [19]. The supplementation of the fermentation medium with organic and inorganic nitrogen sources showed that Bacillus sp.…”

Section: Optimization Of Extracellular Keratinase Activity and Total mentioning

confidence: 92%

Bacillus sp. FPF-1 Produced Keratinase with High Potential for Chicken Feather Degradation

2020

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Chicken feathers are predominantly composed of keratin; hence, valorizing the wastes becomes an imperative. In view of this, we isolated keratinase-producing bacteria and identified them through the 16S rDNA sequence. The process condition for keratinase activity was optimized, and electron micrography of the degradation timelines was determined. Keratinolytic bacteria were isolated and identified as Bacillus sp. FPF-1, Chryseobacterium sp. FPF-8, Brevibacillus sp. Nnolim-K2, Brevibacillus sp. FPF-12 and Brevibacillus sp. FSS-1; and their respective nucleotide sequences were deposited in GenBank, with the accession numbers MG214993, MG214994, MG214995, MG214996 and MG214999. The degree of feather degradation and keratinase concentration among the isolates ranged from 62.5 ± 2.12 to 86.0 ± 1.41(%) and 214.55 ± 5.14 to 440.01 ± 20.57 (U/mL), respectively. In the same vein, 0.1% (w/v) xylose, 0.5% (w/v) chicken feather, an initial fermentation pH of 5.0, fermentation temperature of 25 °C and an agitation speed of 150 rpm, respectively, served as the optimal physicochemical conditions for keratinase activity by Bacillus sp. FPF-1. The time course showed that Bacillus sp. FPF-1 yielded a keratinase concentration of 1698.18 ± 53.99(U/mL) at 120 h. The electron microscopic imaging showed completely structural dismemberment of intact chicken feather. Bacillus sp. FPF-1 holds great potential in the valorization of recalcitrant keratinous biomass from the agro sector into useful products.

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Section: Optimization Of Extracellular Keratinase Activity and Total mentioning

confidence: 92%

Bacillus sp. FPF-1 Produced Keratinase with High Potential for Chicken Feather Degradation

2020

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“…Similarly, feathers hydrolyzed by fermentation with B. subtilis S1-4 was a source of a specific antioxidative peptide [26]. Applicability of feather hydrolysate as soil fertilizer was confirmed by Bose et al [27]. Fermentation of feather waste with B. amyloliquefaciens 6B allowed for obtaining a product effective as agricultural nitrogen input.…”

Section: Introductionmentioning

confidence: 91%

Enzymatic Degradation of Pretreated Pig Bristles with Crude Keratinase of Bacillus cereus PCM 2849

Łaba

Chorążyk

Pudło

et al. 2016

Waste Biomass Valor

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Purpose The aim of the study was to apply and optimize the process of bioconversion of pig bristle waste using keratinolytic enzymes of Bacillus cereus PCM 2849, and to evaluate the amino acid composition of the resultant hydrolysate. Methods Hydrolysis with concentrated culture fluid of B. cereus was applied for bioconversion of pig bristles, after thermo-chemical pretreatment with sulfite. The effect of substrate concentration, sulfite concentration during pretreatment and reaction temperature on the release of amino acids was determined using Box-Behnken design. Amino acid composition of the obtained hydrolysate was determined by HPLC. Structural condition and substructural changes of the residual substrate were evaluated with SEM microscopy and FTIR spectroscopy. Results The applied enzymatic preparation for bristle biodegradation was verified to contain multiple proteases of a wide molecular weight range. A regression model was developed, in which influential parameters were: linear effect of substrate concentration, followed by quadratic effects of reaction temperature, substrate concentration and pretreatment. Optimum reaction conditions were also determined. The resultant hydrolysate was rich in branched-chain amino acids. Residual substrate was detriorated and sulfitolytic cleavage of disulfides and alteration of protein secondary structures was confirmed. Conclusions Application of B. cereus crude keratinase allowed for partial hydrolysis of pig bristles, preceded by sulfitolytic pretreatment. A regression model was built to describe the process of hydrolysis to release free amino acids, at constant enzyme load. Hydrolysis in given conditions allowed to obtain hydrolysate rich in branched chain amino acids. The presented process poses an alternate way of management over pig bristles, a hard-to-degrade keratinous waste.

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“…A aplicação de hidrolisados de penas como fertilizantes possuem efeitos positivos quando comparados à ausência de fertilização e mesmo quando comparados ao uso de fertilizantes comerciais. Resultados promissores vêm sendo descritos na literatura científica para cultivares como couve chinesa (Cao et al, 2012), grão de bico (Paul et al, 2013;Verma et al, 2016), banana (Gurav e Jadhav, 2013), feijão mungo (Bose et al, 2014), trigo (Jain et al, 2016) e arroz (Tamreihao et al, 2017).…”

Section: Ração Animalunclassified

SUB-BOSQUE EM UM TESTE DE PROGÊNIES E PROCEDÊNCIAS DE Eucalyptus urophylla S.T. BLAKE

Cambuim¹,

Pupin²,

Zaruma³

et al. 2020

Sistematização De Uma Agenda Para Questões Socioambientais

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Keratinolytic Protease Production by Bacillus amyloliquefaciens 6B Using Feather Meal as Substrate and Application of Feather Hydrolysate as Organic Nitrogen Input for Agricultural Soil

Cited by 53 publications

References 48 publications

Bacillus sp. FPF-1 Produced Keratinase with High Potential for Chicken Feather Degradation

Bacillus sp. FPF-1 Produced Keratinase with High Potential for Chicken Feather Degradation

Enzymatic Degradation of Pretreated Pig Bristles with Crude Keratinase of Bacillus cereus PCM 2849

SUB-BOSQUE EM UM TESTE DE PROGÊNIES E PROCEDÊNCIAS DE Eucalyptus urophylla S.T. BLAKE

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