Ketamine-xylazine anaesthesia and orofacial administration of substance P: A lethal combination in rats

Francischi, Janetti Nogueira de; Frade, Taíssa Iolanda C.; Almeida, Marcella P.A. de; Queiroz, Bárbara F.G. de; Bakhle, Y.S.

doi:10.1016/j.npep.2017.01.003

Cited by 16 publications

(12 citation statements)

References 25 publications

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“…Recently protective effects of polyphenolic compounds obtained from various diet resources, against oxidative stress resulted from cancer and cancer drugs. Polyphenolic compounds are the most important part of natural plant products with known anti-inflammatory, antimicrobial, anti-alergenic and antioxidant effects (Francischi et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Grape Molasses and Resveratrol Against DMBA Induced Oxidative Stress in Rat Ovarian Tissues

Kıran

Otlu

Karabulut

et al. 2019

Middle Black Sea Journal of Health Science

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The polyphenolic compound, resveratrol (3, 4', 5-trihydroxystilbene), trans-resveratrol is a natural phytoalexin that exists in many different foods such as grape peel and seed, peanut, mulberry and blueberry. Molasses is a natural food obtained by boiling and concentrating fruit juices known to be rich in minerals such as iron, phosphorus, potassium, sodium, calcium and magnesium, and phosphoric acid, formic acid, organic acids and some vitamins. Polyphenolic compounds are natural antioxidants and are known to have protective effects against tissue damage caused by reactive oxygen species (ROS). In this study, we aimed to investigate the protective effects of grape molasses and resveratrol on ovarian damage induced by 7, 12-dimethylbenz [a] anthracene (DMBA). Methods: A total of 42 old female Wistar Albino rats, aged 18 weeks were divided into six groups. 10 mg/kg DMBA was injected in the rats in DMBA group subcutaneously on day 0 and day 7 while grape molasses feed with 20% grape molasses was given along with DMBA application to the rats in DMBA + grape molasses group. Resveratrol was administered by subcutaneous injection at 10 mg/kg/day to the DMBA + Resveratrol group, while only feed with 20% grape molasses was given to the grape molasses group. Resveratrol was administered by subcutaneous injection at 10 mg/kg/day to the resveratrol group. Results: GSH activity between the control group and molasses group, DMBA and DMBA + molasses groups and the control group and the DMBA + grape molasses groups was found statistically significant. Regarding the NO activity, the difference between the DMBA and resveratrol groups, DMBA and DMBA + resveratrol groups and the grape molasses and resveratrol groups was found statistically significant. MDA activity between DMBA + resveratrol and DMBA + grape molasses groups, and DMBA + grape molasses and grape molasses groups was found statistically significant. Conclusion: Molasses as one of the most important nutrient sources of Eastern and Central Anatolia was observed to decrease ovarian tissue oxidative damage induced with DMBA compared to resveratrol.

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Grape Molasses and Resveratrol Against DMBA Induced Oxidative Stress in Rat Ovarian Tissues

Kıran

Otlu

Karabulut

et al. 2019

Middle Black Sea Journal of Health Science

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“…ketamine and 15 mg/kg b.w. xylazine (Francischi et al, 2017) we have shown that injection of carrageenan, a standard proinflammatory stimulus, into the cheek (intra-oral injection. Serum was stored in -80°C until use.…”

Section: Experimental Designmentioning

confidence: 99%

Mahonia Aquifolium Flowers Extract Effects in Acute Experimental Inflammation

Cecan¹,

Pârvu²,

Pârvu³

et al. 2018

BUASVMCN-FST

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Natural products were proved to have inhibitory effect on the nitro-oxidative stress. The aim of the study was to evaluate the effect of Mahonia aquifolium (MA) flowers extract upon nitro-oxidative stress in acute experimental inflammation. The extract was prepared by repercolation method. Acute experimental inflammation was induced with turpentine oil (0,6ml/kg b.w. i.m.). MA extract was given for 7 days. Were used 6 groups (n=5) of male Wistar rats: Groups 1-3 were with acute inflammation and treated with MA dilutions (100%, 50%, 25%); Group 4 was acute inflammation control; Group 5 was negative control; Group 6 was acute inflammation treated with diclofenac (10mg/kg b.w. p.o). In day 8 nitro-oxidative stress was evaluated by measuring serum nitrites and nitrates (NOx), Total oxidative stress (TOS), Total antioxidant capacity (TAC), Oxidative stress index (OSI), Malondialdehyde (MDA) and Thiols (SH). MA reduced OSI and TOS, increased SH, and had no important effect on TAC, NO and MDA. Compared to MA, Diclofenac was a stronger inhibitor of TOS and OSI, and had a smaller effect on SH. Mahonia aquifolium flowers extract had inhibitory effect on the oxidative stress, without influencing NO and lypoperoxides production, the effect being smaller than that of Diclofenac.

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“…He used the most common and basic method of simple randomization by flipping a coin for our two treatment groups (shames versus treatment) which determined the assignment of each subject [1,18,19,22,[34][35][36][37][38][39][40][41][42] Randomization was for 30 animals [37,38,[40][41][42]. Medication was dependent on the rats physical and behavioral stat on the day of testing (1st Op).…”

Section: Synopsis Of the Medicationmentioning

confidence: 99%

“…General anesthesia [39][40][41][42] Preoperatively, all animals were preconditioned for at least one week while being housed in the veterinarian laboratories to become familiar with the new environment and staff, such as the stockman and surgical team, regarding their actual and intended special manipulations [34][35][36]40], e.g. feeding and drinking, being taken out of the box and placed and fixed on the operating table, being placed into a veterinarian CO 2 insufflation box for sedation prior to the general anesthesia procedures for the 1st, 2nd, and 3rd operations [35,36,[39][40][41].…”

Section: Synopsis Of the Medicationmentioning

confidence: 99%

“…feeding and drinking, being taken out of the box and placed and fixed on the operating table, being placed into a veterinarian CO 2 insufflation box for sedation prior to the general anesthesia procedures for the 1st, 2nd, and 3rd operations [35,36,[39][40][41]. A synopsis of the indications for all pharmaceuticals and biochemical products, their structures, applied dosages and routes of administration, and the different producers are summarized in Table 1 [34][35][36][37][38][39][40]. Cerebrolysin ® peptidergic drug is composed of porcine brain proteins, with a mixture of 75% free amino acid and 25% lowmolecular-weight peptides and is produced by EVER Neuro Pharma GmbH, A-4866 Unterach, A [1,18,19,22,23].…”

Section: Synopsis Of the Medicationmentioning

confidence: 99%

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New Aspects of Graft-induced Central Plasticity and Neuroregeneration at T 10 in a Rat Model†

Wild¹,

Cătoi²,

Wild³

et al. 2017

J Neurol Neurophysiol

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Objective: Retest Brunelli's graft induced glutamate neurotransmitter switch at the neuromuscular junction in rat for the translation of new aspects of central plasticity concepts for human reconstructive surgery in spinal cord lesions. Methods:Randomized double blind controlled study in rat, which was limited to 30 animals (Charles River, 220 to 280 g). Ethical research approval was obtained from the Animal Research Committee of the University Hospital Schleswig Holstein, UK-SH, Lübeck, D, and legitimated by the Governmental Department of Agriculture, Natural Environments and Agriculture Kiel, D, in compliance with the European Commission Recommendation to retest and review of graft-induced glutamatergic regeneration and /or cholinergic co-transmission at the neuromuscular junction for reinnervation of the skeletal internal obliquus abdominal muscle fibres. Assessments were performed to demonstrate pharmacological neuromodulation after attaching the lateral corticospinal tract at T10 to the bisected skeletal motor nerve. Medication was administered for 14 days postoperatively-a) verum Cerebrolysin ® IP=12-b) shams NaCl 0.9% IP=11, and c) 7 controls (nil). 2nd Op at day 90 (16 surviving rats) for open proof of reinnervation and its type by a) CMAP, b)Vecuronium ® application. Fast Blue ® labeling were performed. 10 days later, on the 3rd Op N=15, euthanasia and organ fixation were performed. Extensive histology-morphology examinations were performed in Cluj.Results: Eight rats showed positive CMAPs. Reinnervation and neuromodulation were demonstrated by counting and comparison of the grafted muscle fibers diameter. Four CAMP-positive-rats received Vecuronium ® : 1 CERE and 1 NaCl each demonstrated a loss of amplitude respectively two an incomplete muscle blockage due to the coexistence of glutamatergic and cholinergic neurotransmission. Confirmation of the VGluT2 in axons was observed by immunofluorescence. FB+ neurons were observed in many Rexed laminae in grafted spinal cord, but not in the brain. Conclusion:The coexistence of graft-induced cholinergic and glutamatergic neurotransmission and a great capacity of lower motor neurons and other types of spinal neurons to regenerate were observed. Because of limited animals, pharmacological neuromodulation requires further investigation.

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Ketamine-xylazine anaesthesia and orofacial administration of substance P: A lethal combination in rats

Cited by 16 publications

References 25 publications

Protective Effects of Grape Molasses and Resveratrol Against DMBA Induced Oxidative Stress in Rat Ovarian Tissues

Protective Effects of Grape Molasses and Resveratrol Against DMBA Induced Oxidative Stress in Rat Ovarian Tissues

Mahonia Aquifolium Flowers Extract Effects in Acute Experimental Inflammation

New Aspects of Graft-induced Central Plasticity and Neuroregeneration at T 10 in a Rat Model†

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