Ketone body and acetate formation from oleate by isolated rat testicular cells

Yount, Emily A.; Harris, Robert A.

doi:10.1016/0003-9861(82)90531-8

Cited by 14 publications

(6 citation statements)

References 31 publications

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“…Previous studies of fatty acid oxidation in crude testicular cell suspensions have been reported (Lin & Fritz, 1972;Yount & Harris, 1982). Lin & Fritz (1972) concluded that spermatogonia oxidized palmitate to C02 and acetoacetate.…”

Section: Discussionmentioning

confidence: 99%

“…However, it cannot be excluded from their results that the oxidation took place in Sertoli cells which are also present in such cell suspensions. Furthermore, the decrease in oxidation of fatty acids in cell suspensions from testes from rats of increasing ages (Lin & Fritz, 1972;Yount & Harris, 1982) (Page, Krebs & Williamson, 1971).…”

Section: Discussionmentioning

confidence: 99%

“…These lipid droplets contain triacylglycerols which upon lipolysis generate glycerol and free fatty acids. Investigations of fatty acid oxidation with biochemical methods have been performed only with testicular cell suspensions (Lin & Fritz, 1972;Yount & Harris, 1982), and these suspensions contain many different cell types which may contribute to the metabolism. (BSA, Sigma).…”

Section: Introductionmentioning

confidence: 99%

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Metabolism of palmitate in cultured rat Sertoli cells

Jutte¹,

Eikvar²,

Levy³

et al. 1985

Reproduction

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolism of palmitate in cultured rat Sertoli cells

Jutte¹,

Eikvar²,

Levy³

et al. 1985

Reproduction

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“…Glycerol phosphate dehydrogenase activity was not affected by any of the hormones tested. When Sertoli cells from 19-day old rats were incubated with [1,[2][3][4][5][6][7][8][9][10][11][12][13][14] C]acetate for 90 or 360 min, the [ 14 C] label was present predominantly in triglyceride and phospholipid fractions with minor amounts in other lipids. In Sertoli cells pretreated for 16 h with insulin and FSH, an increase in acetate incorporation into lipids was observed.…”

Section: Introductionmentioning

confidence: 99%

“…The metabolism of oleate in isolated rat testicular cells was investigated by Yount and Harris (3). The rates of oxidation of oleate to carbon dioxide were higher in cells preparared from immature rats than in cells from adults.…”

Section: Introductionmentioning

confidence: 99%

Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats

Guma¹,

Wagner²,

Martini³

et al. 1997

Braz J Med Biol Res

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Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 µg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD + formed µg protein -1 min -1 . FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate dehydrogenase activity was not affected by any of the hormones tested. When Sertoli cells from 19-day old rats were incubated with [1,2-14 C]acetate for 90 or 360 min, the [ 14 C] label was present predominantly in triglyceride and phospholipid fractions with minor amounts in other lipids. In Sertoli cells pretreated for 16 h with insulin and FSH, an increase in acetate incorporation into lipids was observed. Most of the label was in esterified lipids and this percentage increased with the time of treatment; this increase was remarkable in triglycerides of control cells (18.8% to 30.6%). Since Sertoli cell triglycerides participate in the control of spermatogenesis, the present data suggest that the hormonal control of lipid metabolism in Sertoli cells is important not only for maintaining the energy of the cell itself, but also for the control of the spermatogenesis process.

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The Warburg Effect Revisited—Lesson from the Sertoli Cell

Oliveira

Martins

Moreira

et al. 2014

Medicinal Research Reviews

151

113

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Otto Warburg observed that cancerous cells prefer fermentative instead of oxidative metabolism of glucose, although the former is in theory less efficient. Since Warburg’s pioneering works, special attention has been given to this difference in cell metabolism. The Warburg effect has been implicated in cell transformation, immortalization, and proliferation during tumorigenesis. Cancer cells display enhanced glycolytic activity, which is correlated with high proliferation, and thus, glycolysis appears to be an excellent candidate to target cancer cells. Nevertheless, little attention has been given to noncancerous cells that exhibit a “Warburg-like” metabolism with slight, but perhaps crucial, alterations that may provide new directions to develop new and effective anticancer therapies. Within the testis, the somatic Sertoli cell (SC) presents several common metabolic features analogous to cancer cells, and a clear “Warburg-like” metabolism. Nevertheless, SCs actively proliferate only during a specific time period, ceasing to divide in most species after puberty, when they become terminally differentiated. The special metabolic features of SC, as well as progression from the immature but proliferative state, to the mature nonproliferative state, where a high glycolytic activity is maintained, make these cells unique and a good model to discuss new perspectives on the Warburg effect. Herein we provide new insight on how the somatic SC may be a source of new and exciting information concerning the Warburg effect and cell proliferation.

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Ketone body and acetate formation from oleate by isolated rat testicular cells

Cited by 14 publications

References 31 publications

Metabolism of palmitate in cultured rat Sertoli cells

Metabolism of palmitate in cultured rat Sertoli cells

Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats

The Warburg Effect Revisited—Lesson from the Sertoli Cell

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