Metabolism of palmitate in cultured rat Sertoli cells

Jutte, N. H. P. M.; Eikvar, Lars; Levy, Finn Olav; Hansson, V.

doi:10.1530/jrf.0.0730497

Cited by 37 publications

(32 citation statements)

References 16 publications

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“…An indication that lipids may be a more important energy substrate than glucose for Sertoli cells was provided by Jutte et al (2) who showed that Sertoli cells oxidize palmitate in a concentration-dependent manner and that the addition of glucose to the incubation system does not change palmitate oxidation but causes a 9-fold increase in palmitate esterification to TG, probably due to an increase in glycerol-3-phosphate levels. Fisher and Coniglio (21) demonstrated that the amount of radioactivity in the TG of Sertoli cells was constant between 24 and 48 h but was decreased in PL, suggesting a higher FA turnover in PL than in TG.…”

Section: Discussionmentioning

confidence: 99%

“…The metabolism of [ 14 C]palmitic acid in primary cultures of rat Sertoli cells isolated from testes of 19-day old rats has been reported (2). The oxidation of palmitate was concentration dependent.…”

Section: Introductionmentioning

confidence: 99%

“…The addition of glucose had no effect on palmitate oxidation but caused a 9-fold increase in esterification into triacylglycerols. The authors concluded that fatty acids appear to be a major energy substrate for Sertoli cells (2).…”

Section: Introductionmentioning

confidence: 99%

“…Glycerol phosphate dehydrogenase activity was not affected by any of the hormones tested. When Sertoli cells from 19-day old rats were incubated with [1,[2][3][4][5][6][7][8][9][10][11][12][13][14] C]acetate for 90 or 360 min, the [ 14 C] label was present predominantly in triglyceride and phospholipid fractions with minor amounts in other lipids. In Sertoli cells pretreated for 16 h with insulin and FSH, an increase in acetate incorporation into lipids was observed.…”

Section: Introductionmentioning

confidence: 99%

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Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats

Guma¹,

Wagner²,

Martini³

et al. 1997

Braz J Med Biol Res

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Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 µg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD + formed µg protein -1 min -1 . FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate dehydrogenase activity was not affected by any of the hormones tested. When Sertoli cells from 19-day old rats were incubated with [1,2-14 C]acetate for 90 or 360 min, the [ 14 C] label was present predominantly in triglyceride and phospholipid fractions with minor amounts in other lipids. In Sertoli cells pretreated for 16 h with insulin and FSH, an increase in acetate incorporation into lipids was observed. Most of the label was in esterified lipids and this percentage increased with the time of treatment; this increase was remarkable in triglycerides of control cells (18.8% to 30.6%). Since Sertoli cell triglycerides participate in the control of spermatogenesis, the present data suggest that the hormonal control of lipid metabolism in Sertoli cells is important not only for maintaining the energy of the cell itself, but also for the control of the spermatogenesis process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats

Guma¹,

Wagner²,

Martini³

et al. 1997

Braz J Med Biol Res

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show abstract

“…Cultured rat SCs rely in fatty acids oxidation to sustain their energetic needs [67]. Moreover, they phagocytize and degrade apoptotic spermatogenic cells and residual bodies to form lipids that can be then re-used to produce ATP during spermatogenesis [68].…”

Section: Metabolic Cooperation In Testis Is Piv-otal For Spermatogenesismentioning

confidence: 99%

Metabolic Cooperation in Testis as a Pharmacological Target: From Disease to Contraception

Alves¹,

Dias²,

Silva³

et al. 2015

CMP

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Abstract:The development of a "male pill" to control fertility is still a major challenge. Although women have several options to play an active role in the couple family planning, men are very limited in terms of contraceptive methods. Several approaches have been proposed to develop a male contraceptive and can be divided in two major groups: hormonal and non-hormonal methods. Within the testis, the somatic Sertoli cell (SC) is known as the "nurse cell" since it provides the physical and nutritional support for the developing germ cells. Moreover, adjacent SCs form the Sertoli/blood testis barrier (BTB), which divides the seminiferous epithelium into the basal and the apical compartments, controlling the passage of substances to the site where germ cells develop. Among the several functions of SC, its metabolism and the production of lactate, acetate and other metabolic factors are essential for the normal occurrence of spermatogenesis. In the last years, several works have highlighted that the metabolic cooperation established between SCs and developing germ cells is compromised in several diseases associated with male subfertility/infertility. Notably, several metabolismassociated proteins are specifically expressed in the testis. Thus, there are several evidences illustrating that the control of male fertility can be achieved by targeting testicular cells metabolism. Herein, we discuss the metabolic cooperation in testis as a potential pharmacological target to counteract subfertility/infertility promoted by several diseases, particularly metabolic diseases. We also discuss how it can contribute to the development of a male contraceptive.

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Sertoli cell adaptation to glucose deprivation: Potential role of AMPK in the regulation of lipid metabolism

Rindone

Dasso

Centola

et al. 2023

J of Cellular Biochemistry

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Sertoli cells (SCs) provide an adequate environment for germ cell development. SCs possess unique features that meet germ cells' metabolic demands: they produce lactate from glucose, which is delivered as energy substrate to germ cells. SCs store fatty acids (FAs) as triacylglycerols (TAGs) in lipid droplets (LDs) and can oxidize FAs to sustain their own energetic demands. They also produce ketone bodies from FAs. It has been shown that exposure of SCs to metabolic stresses, such as glucose deprivation, triggers specific adaptive responses that sustain cell survival and preserve lactate supply to germ cells. The aim of the present study was to investigate whether there are modifications in rat SCs lipid metabolism, including LD content, FA oxidation, and ketone bodies production, as part of their adaptive response to glucose deprivation. The present study was performed in 20‐day‐old rat SCs cultures. We determined LD content by Oil Red O staining, FA oxidation by measuring the release of 3H2O from [3H] palmitate, TAGs and 3‐hydroxybutyrate levels by spectrophotometric methods, and mRNA levels by RT‐qPCR. Results show that the absence of glucose in SC culture medium entails: (1) a decrease in LD content and TAGs levels that is accompanied by decreased perilipin 1 mRNA levels, (2) an increase in FA oxidation that is in part mediated by AMP kinase (AMPK) activation and (3) a decrease in 3‐hydroxybutyrate production. Additionally, we studied whether sestrins (SESN1, 2 and 3), proteins involved in the cellular response to stress, are regulated in glucose deprivation conditions. We show that there is an increase in SESN2 mRNA levels in deprived conditions. In conclusion, glucose deprivation affects SC lipid metabolism promoting FA mobilization from LDs to be used as energy source.

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Metabolism of palmitate in cultured rat Sertoli cells

Abstract: Summary. Isolated rat Sertoli cells were incubated in the presence of [1-14C]

Cited by 37 publications

References 16 publications

Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats

Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats

Metabolic Cooperation in Testis as a Pharmacological Target: From Disease to Contraception

Sertoli cell adaptation to glucose deprivation: Potential role of AMPK in the regulation of lipid metabolism

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