Key adhesion molecules are present on long podia extended by hematopoietic cells

Holloway, William; Martinez, Alexander R.; Oh, Deok-Kun; Francis, Karl; Ramakrishna, Ramprasad; Palsson, Bernhard Ø.

doi:10.1002/(sici)1097-0320(19991101)37:3<171::aid-cyto2>3.0.co;2-8

Cited by 23 publications

(20 citation statements)

References 29 publications

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“…4). This is consistent with previous observations that KG1a extend fillopodia during migration which express b1 and b2 integrins [32]. The role of b1 and b2 integrins in recruitment to the liver is consistent with the findings of Jin and colleagues [36] who reported that CD34 + bone marrow progenitors use a4b1 integrin to bind VCAM-1 and fibronectin in the neovasculature during recruitment to remodeling tissues.…”

Section: Discussionsupporting

confidence: 92%

“…Previous studies have shown that KG1a, PB and MB express CD11a, CD18, CD29, CD49d and CD49e [32,36], in agreement with our data and lower levels of CD11a are associated with mobilization from bone marrow without modulating subsequent engraftment [39]. In our study, CD11a expression on MB was between the levels expressed on KG1a and PB populations (37%, 25% and 50% respectively) but lower than mentioned in previous studies [39].…”

Section: Discussionsupporting

confidence: 92%

“…This led us to compare the phenotype and functional behavior of the CD34 + cell line KG1a with MB and PB CD34 + cells because this cell line provides a ready supply of homogeneous CD34 + cells. KG1a cells are a human acute myeloid leukemia cell line comprised of undifferentiated blast cells previously shown to express CD34, CD44 and CXCR4 [31][32][33]. We carried out an extensive phenotypic analysis of adhesion receptors and chemokine receptors on these cells to see if they could act as a model stem cell to allow us to carry out definitive adhesion and migration studies in vitro.…”

Section: Discussionmentioning

confidence: 99%

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Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4

Crosby¹,

Ross²,

Newsome³

et al. 2009

Journal of Hepatology

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4

Crosby¹,

Ross²,

Newsome³

et al. 2009

Journal of Hepatology

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“…In addition to their classical projections, it has also been reported that hematopoietic cells can display a variety of dynamic pseudopods of diverse morphology (7,8). Tenupodia are very thin, adhere to the plate surface, and can bifurcate multiple times.…”

Section: Introductionmentioning

confidence: 99%

“…Both types of podia can exceed 300 μm in length. Such structures are believed to participate in cell navigation and cell-cell communication (7,8).…”

Section: Introductionmentioning

confidence: 99%

Novel membrane cell projection defects in Wiskott-Aldrich syndrome B cells

Andreu

Aran²,

Fillat³

2007

Int J Mol Med

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Abstract. Wiskott-Aldrich syndrome (WAS) is an X-linked immunodeficiency characterized by microthrombocytopenia, eczema, recurrent infections, autoimmune disorders and an increased incidence of malignancies. This complex phenotype results from mutations in the WASP gene. WASP is a key member of a protein family that links signaling pathways to actin cytoskeleton reorganization by activating Arp2/3-mediated actin polymerization. Actin polymerization defects have been extensively defined in WAS T cells and also in dendritic cells and macrophages, but few reports have concentrated on WAS B cells. In the present study, we investigated cytoskeleton abnormalities in WAS B cell lines. For the first time we report alterations in the capacity of these cells to extend filopodia in response to bradykinin stimuli and an impairment in the formation of long pseudopodia under basal conditions. Such alterations most probably result from a WASP dysfunction, given that a retroviral gene transfer of a corrected form of the WASP gene was able to rescue the abnormal phenotypes.

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Extension of osmolality-induced podia is observed from fluorescently labeled hematopoietic cell lines in hyperosmotic medium

Oh¹,

Martinez²,

Lee³

et al. 2000

Cytometry

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Background Since the description of long podia extended by hematopoietic cells and cell lines, the reliable elicitation of podia extensions is needed to study these podia systemically. In this study, hyperosmotic stress was considered as an elicitor. Methods Using two fluorescent membrane dyes PKH2 and PKH26, and an automated fluorescence microscopy system, morphological changes of seven human cell lines (six hematopoietic, one fibrosarcoma) at different osmolalities were monitored. Presence of surface molecules on the hyperosmolality‐induced podia (osmopodia) was examined. Results In hyperosmotic medium, cells shrank rapidly, followed by osmopodia extension. Cells exhibited variable number (up to five) and length (up to longer than 100 μm) of osmopodia in about 1 h. Dead cells did not extend podia. Frequency, length, and number of podia were variable among cell lines studied. CD44 and CD45 were not present on the osmopodia, although they were present on the cell surface, showing that osmopodia characteristics differ from the podia observed previously in isotonic media. The osmopodia extension process was shown to be reversible upon repeated osmolality changes. Conclusions Osmopodia extended by human hematopoietic cell lines display a newly observed cellular morphology and provide a tool for investigation of dynamic cellular response to environmental changes. Cytometry 40:109–118, 2000. © 2000 Wiley‐Liss, Inc.

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Key adhesion molecules are present on long podia extended by hematopoietic cells

Cited by 23 publications

References 29 publications

Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4

Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4

Novel membrane cell projection defects in Wiskott-Aldrich syndrome B cells

Extension of osmolality-induced podia is observed from fluorescently labeled hematopoietic cell lines in hyperosmotic medium

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