Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria

Boritsch, Eva C.; Khanna, Varun; Pawlik, Alexandre; Honoré, Nadine; Navas, Victor H.; Ma, Laurence; Bouchier, Christiane; Seemann, Torsten; Supply, Philip; Stinear, Timothy P.; Brosch, Roland

doi:10.1073/pnas.1604921113

Cited by 101 publications

(86 citation statements)

References 43 publications

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“…In the MTBC, M. canettii is most closely related to the vanished ancestor (1)(2)(3)(4)(5). M. canettii is uniquely characterized by a large (4.48 Ϯ 0.05 Mb) and mosaic genome with traces of intraspecies horizontal gene transfer (HGT) (3,5,6), including M. tuberculosis-specific deletion 1 (TbD1), which is deleted in "modern" M. tuberculosis lineages (7). In culture, M. canettii appears as large and cordless mycobacteria, and it forms smooth colonies (3,8,9).…”

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confidence: 99%

Ready Experimental Translocation of Mycobacterium canettii Yields Pulmonary Tuberculosis

et al. 2017

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Mycobacterium canettii, which has a smooth colony morphology, is the tuberculous organism retaining the most genetic traits from the putative last common ancestor of the rough-morphology Mycobacterium tuberculosis complex. To explore whether M. canettii can infect individuals by the oral route, mice were fed phosphate-buffered saline or 10 6 M. canettii mycobacteria and sacrificed over a 28-day experiment. While no M. canettii was detected in negative controls, M. canettiiinfected mice yielded granuloma-like lesions for 4/4 lungs at days 14 and 28 postinoculation (p.i.) and positive PCR detection of M. canettii for 5/8 mesenteric lymph nodes at days 1 and 3 p.i. and 5/6 pooled stools collected from day 1 to day 28 p.i. Smooth M. canettii colonies grew from 68% of lungs and 36% of spleens and cervical lymph nodes but fewer than 20% of axillary lymph nodes, livers, brown fat samples, kidneys, or blood samples throughout the 28-day experiment. Ready translocation in mice after digestive tract challenge demonstrates the potential of ingested M. canettii organisms to relocate to distant organs and lungs. The demonstration of this relocation supports the possibility that populations may be infected by environmental M. canettii.

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Ready Experimental Translocation of Mycobacterium canettii Yields Pulmonary Tuberculosis

et al. 2017

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“…In support of these findings, a recent study by Boritsch et al indicated that a similar process of horizontal gene transfer was observed in Mycobacterium canettii, a close relative of M. tuberculosis. Although the dependence of the observed DNA transfer on the ESX-1 and ESX-4 systems was not tested, this study indicates that horizontal gene transfer is likely widespread in mycobacteria (69).…”

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confidence: 83%

Esx Systems and the Mycobacterial Cell Envelope: What's the Connection?

Bosserman

Champion

2017

J Bacteriol

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Mycobacterial 6-kDa early secreted antigenic target (ESAT-6) system (ESX) exporters transport proteins across the cytoplasmic membrane. Many proteins transported by ESX systems are then translocated across the mycobacterial cell envelope and secreted from the cell. Although the mechanism underlying protein transport across the mycolate outer membrane remains elusive, the ESX systems are closely connected with and localize to the cell envelope. Links between ESX-associated proteins, cell wall synthesis, and the maintenance of cell envelope integrity have been reported. Genes encoding the ESX systems and those required for biosynthesis of the mycobacterial envelope are coregulated. Here, we review the interplay between ESX systems and the mycobacterial cell envelope.

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“…These genes may be localized in different genomic positions in the various mycobacterial species concerned. The molecular tools that have helped to establish these putative ancestral horizontal transfer events of the espACD operons into slow‐growing mycobacterial species are unknown, but could have involved recently identified plasmid‐mediated (Dumas et al ., ) and/or chromosomal mycobacterial DNA transfer mechanisms (Boritsch et al ., ).…”

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confidence: 97%

Discovery of the type VII ESX‐1 secretion needle?

Ates

Brosch

2016

Molecular Microbiology

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Summary Mycobacterium tuberculosis, the etiological agent of human tuberculosis, harbours five ESAT‐6/type VII secretion (ESX/T7S) systems. The first esx gene clusters were identified during the genome‐sequencing project of M. tuberculosis H37Rv. Follow‐up studies revealed additional genes playing important roles in ESX/T7S systems. Among the latter genes, one can find those that encode Pro‐Glu (PE) and Pro‐Pro‐Glu (PPE) proteins as well as a gene cluster that is encoded >260 kb upstream of the esx‐1 locus and encodes ESX‐1 secretion‐associated proteins EspA (Rv3616c), EspC (Rv3615c) and EspD (Rv3614c). The espACD cluster has been suggested to have an important function in ESX‐1 secretion since EspA‐EspC and EsxA–EsxB are mutually co‐dependent on each other for secretion. However, the molecular mechanism of this co‐dependence and interaction between the substrates remained unknown. In this issue of Molecular Microbiology, Lou and colleagues show that EspC forms high‐molecular weight polymerization complexes that resemble selected components of type II, III and/or IV secretion systems of Gram‐negative bacteria. Indeed, EspC‐multimeric complexes form filamentous structures that could well represent a secretion needle of ESX‐1 type VII secretion systems. This exciting observation opens new avenues for research to discover and characterize ESX/T7S components and elucidates the co‐dependence of EsxA/B secretion with EspA/C.

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Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria

Cited by 101 publications

References 43 publications

Ready Experimental Translocation of Mycobacterium canettii Yields Pulmonary Tuberculosis

Ready Experimental Translocation of Mycobacterium canettii Yields Pulmonary Tuberculosis

Esx Systems and the Mycobacterial Cell Envelope: What's the Connection?

Discovery of the type VII ESX‐1 secretion needle?

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