Key factors affecting photoactivated fungicidal activity of sodium pheophorbide a against Pestalotiopsis neglecta

Pheophorbide-a as a Light-Triggered Liposomal Switch: For the Controlled Release of Alpinia galanga (A. galanga) Essential Oil and Its Stability, Antioxidant, and Antibacterial Activity Assessment

“…It can be seen from the UV spectrum in Figure A that Pheophorbide-a shows five characteristic absorption peaks in the region of 400–800 nm: a strong Soret absorption peak at 417 nm; a strong Soret absorption peak at 508 nm. There are three weak absorption peaks at nm, 537 and 608 nm; one at 665 nm, which is similar to the spectrum of chlorophyllin-a reported by Kim et al…”

“…It can be seen from the UV spectrum in Figure 2A that Pheophorbide-a shows five characteristic absorption peaks in the region of 400−800 nm: a strong Soret absorption peak at 417 nm; a strong Soret absorption peak at 508 nm. There are three weak absorption peaks at nm, 537 and 608 nm; one at 665 nm, 16 which is similar to the spectrum of chlorophyllin-a reported by Kim et al 11 Due to the intense penetration of light above 630 nm, Pheophorbide-a is a photosensitizer with broad application prospects. The samples were exposed to laser irradiation to evaluate the photodynamic effects of pheophytin in vitro.…”

“…The results further proved that the photosensitivity of the light-responsive liposomes was derived from Pheophorbide-a. According to the theory of photosensitization, photodynamic reactions are mainly divided into two categories: type I refers to the formation of hydroxyl radicals and other reactive oxygen species; type II refers to the formation of singlet oxygen . The reaction of Pheophorbide-a is a type II mechanism of action.…”

Unraveling the polypharmacology of a natural antifungal product, eugenol, against Rhizoctonia solani

“…68,69 In addition, eugenol increased the MDA content, which indicated that lipid peroxidation had occurred in R. solani, exacerbating damage to the membrane and inhibiting mycelial growth. 49 MMP is caused by the uneven distribution of ions inside and outside the mitochondrial membrane. It is of great significance in maintaining the stability and permeability of the mitochondrial membrane and regulating the function of the mitochondria.…”

“…Detection of the ergosterol content of mycelia was conducted using the method published by Gao et al 48 Malondialdehyde (MDA) was measured by the thiobarbituric acid method. 49 Mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) were measured using fluorescent dye Rhodamine 123 and 2 0 ,7 0 -dichlorodihydrofluorescein diacetate, respectively. 50,51 The activities of the superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were measured using CAT, SOD, POD kits (Beijing Solarbio).…”