Key Factors Affecting the Recalcitrance and Conversion Process of Biomass

Melati, Ranieri Bueno; Shimizu, Felipe Lange; Oliveira, G. A. C.; Pagnocca, Fernando Carlos; Souza, Wanderley de; Sant’Anna, Celso; Brienzo, Michel

doi:10.1007/s12155-018-9941-0

Cited by 82 publications

(30 citation statements)

References 150 publications

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“…[1,3,[10][11][12] The lack of well-established processes that add value to lignin can be largely attributed to its structural complexity and associated chemical recalcitrance. [8,[13][14][15] The use of lignin as a feedstock for the production of bio-based chemicals has received increasing interest. [16][17][18][19][20][21] The development of efficient processes for lignin valorization presents an important potential to improve the eco-nomic feasibility and overall sustainability of both pulp and paper processing and cellulosic-based ethanol biorefineries.…”

Section: Introductionmentioning

confidence: 99%

Lignin Depolymerization: A Comparison of Methods to Analyze Monomers and Oligomers

et al. 2020

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Catalytic liquefaction of lignin is an attractive process to produce fuels and chemicals, but it forms a wide range of liquid products from monomers to oligomers. Oligomers represent an important fraction of the products and their analysis is complex. Therefore, rapid characterization methods are needed to screen liquefaction conditions based on the distribution in monomers and oligomers. For this purpose, UV spectroscopy is proposed as a fast and simple method to assess the composition of lignin‐derived liquids. UV absorption and fluorescence were studied on various model compounds and liquefaction products. Liquefaction of Soda lignin was conducted in an autoclave, in ethanol and with Pt/C catalyst (H2, 250 °C, 110 bar). Liquids were sampled at isothermal conditions every 30 min for 4 h. UV fluorescence spectroscopy is related to GC–MS, gel‐permeation chromatography (GPC), MALDI‐TOF MS, and NMR characterizations. A depolymerization index is proposed from UV spectroscopy to rapidly assess the relative distribution of monomers and oligomers.

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Section: Introductionmentioning

confidence: 99%

Lignin Depolymerization: A Comparison of Methods to Analyze Monomers and Oligomers

et al. 2020

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“…Acetic acid concentration varied from 1.02 g L −1 (16‐mesh bagasse) to 0.52 g L −1 (base). The highest sugar solubilization was from the hemicellulose components (xylose, arabinose and acetic acid), which is a characteristic of diluted acid pretreatment 1 . The xylan components were reported in higher concentration than the ones from cellulose in the liquid hydrolysate 27 .…”

Section: Resultsmentioning

confidence: 96%

“…Lignocellulosic biomass is an abundant and low‐cost material, which represents an advantage to biorefinery processes 1 . This material is composed of cellulose, hemicellulose (xylan) and lignin, with lower amounts of ash and extractives 2 .…”

Section: Introductionmentioning

confidence: 99%

Sieving process selects sugarcane bagasse with lower recalcitrance to xylan solubilization

Alves

Melati

Casagrande

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUNDSugarcane bagasse is a heterogeneous and organized material that needs a pretreatment to access the polysaccharides (cellulose and xylan). The particle size of a non‐milled bagasse can influence the xylan and xylose solubilization, as well as the enzymatic hydrolysis of the pretreated material. Using bagasse selected on 16‐, 30‐, 40‐ and 50‐mesh sieves: xylan solubilization was performed with hydrogen peroxide; xylose was solubilized with diluted sulfuric acid; and enzymatic hydrolysis was conducted for glucose yield evaluation.RESULTSThe xylose solubilized by acid pretreatment resulted in a maximum of 6.59 g L−1 with no influence of particle size. The solubilized xylan showed linear correlation with a variation of 36.18% (16‐mesh bagasse) to 71.43% (<50‐mesh bagasse). Pretreated material submitted to enzymatic hydrolysis revealed that particle size influenced the pretreatment, increasing the glucose yield.CONCLUSIONSThe results showed that non‐milled sugarcane bagasse particle size selection positively influenced the solubilization of xylan, improving the enzymatic hydrolysis of the pretreated material. © 2020 Society of Chemical Industry (SCI)

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“…These side chains contribute to the recalcitrancy of plant biomass due to its diverse structural composition 2,35 . The removal of hemicellulose makes it less recalcitrant, improving the hydrolysis process 8,9 . For this purpose, enzymes such as arabinofuranosidases that act on these sugars are necessary 34 .…”

Section: Discussionmentioning

confidence: 99%

“…For the enzymes to access the main chain of hemicellulose, it is necessary to remove side-chain decorations, as they are one of the main reasons for the recalcintrance of plant biomass 8,9 . Thus, the search for enzymes that target hemicellulose decorations is a promising approach for improving the saccharification of lignocellulolytic material 10 .…”

Section: Introductionmentioning

confidence: 99%

Novel fungal metal-dependent GH54 α-L-arabinofuranosidase: expanded substrate specificity and potential use for plant biomass degradation

Motta

Filho

Melo

et al. 2020

Preprint

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Trichoderma genus fungi present great potential for the production of carbohydrate-active enzymes (CAZYmes), including glycoside hydrolase (GH) family members. From a renewability perspective, CAZYmes can be biotechnologically exploited to convert plant biomass into free sugars for the production of advanced biofuels and other high-value chemicals. GH54 is an attractive enzyme family for biotechnological applications because many GH54 enzymes are bifunctional. Thus, GH54 enzymes are interesting targets in the search for new enzymes for use in industrial processes such as plant biomass conversion. Herein, a novel metal-dependent GH54 arabinofuranosidase (ThABF) from the cellulolytic fungus Trichoderma harzianum was identified and biochemically characterized. Initial in silico searches were performed to identify the GH54 sequence. Next, the gene was cloned and heterologously overexpressed in Escherichia coli. The recombinant protein was purified, and the enzyme’s biochemical and biophysical properties were assessed. The GH54 members show wide functional diversity and specifically remove plant cell decorations including arabinose and galactose, in the presence of a metallic cofactor. Plant cell wall decoration have a major impact on lignocellulosic substrate conversion into high-value chemicals. These results expand the known functional diversity within the GH54 family, showing the potential of a novel arabinofuranosidase for plant biomass degradation.

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Key Factors Affecting the Recalcitrance and Conversion Process of Biomass

Cited by 82 publications

References 150 publications

Lignin Depolymerization: A Comparison of Methods to Analyze Monomers and Oligomers

Lignin Depolymerization: A Comparison of Methods to Analyze Monomers and Oligomers

Sieving process selects sugarcane bagasse with lower recalcitrance to xylan solubilization

Novel fungal metal-dependent GH54 α-L-arabinofuranosidase: expanded substrate specificity and potential use for plant biomass degradation

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