Key lipid molecules in hepatopancreas of
            <i>Eriocheir sinensis</i>
            : Identification and thermal oxidative degradation characteristics

Yin, Mingyu; Fu, Xueyan; Wang, Xichang

doi:10.1111/jfbc.13734

Cited by 6 publications

(1 citation statement)

References 34 publications

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“…Although previous studies have highlighted the crucial roles exerted by carotenoid oxygenases and isomerases in the carotenoid metabolism of animals, their functions remain largely unclear in crustaceans. Chinese mitten crab E. sinensis is an important economic crustacean; it is particularly popular due to its delicate flavor, red carapace, and deep yellow hepatopancreas [ 25 , 26 , 27 ]. With regard to the edible tissues of E. sinensis , its hepatopancreas and ovaries are rich in carotenoids [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

NinaB and BCO Collaboratively Participate in the β-Carotene Catabolism in Crustaceans: A Case Study on Chinese Mitten Crab Eriocheir sinensis

Zhang,

Xiong,

Yang

et al. 2024

IJMS

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Carotenoid cleavage oxygenases can cleave carotenoids into a range of biologically important products. Carotenoid isomerooxygenase (NinaB) and β, β-carotene 15, 15′-monooxygenase (BCO1) are two important oxygenases. In order to understand the roles that both oxygenases exert in crustaceans, we first investigated NinaB-like (EsNinaBl) and BCO1-like (EsBCO1l) within the genome of Chinese mitten crab (Eriocheir sinensis). Their functions were then deciphered through an analysis of their expression patterns, an in vitro β-carotene degradation assay, and RNA interference. The results showed that both EsNinaBl and EsBCO1l contain an RPE65 domain and exhibit high levels of expression in the hepatopancreas. During the molting stage, EsNinaBl exhibited significant upregulation in stage C, whereas EsBCO1l showed significantly higher expression levels at stage AB. Moreover, dietary supplementation with β-carotene resulted in a notable increase in the expression of EsNinaBl and EsBCO1l in the hepatopancreas. Further functional assays showed that the EsNinaBl expressed in E. coli underwent significant changes in its color, from orange to light; in addition, its β-carotene cleavage was higher than that of EsBCO1l. After the knockdown of EsNinaBl or EsBCO1l in juvenile E. sinensis, the expression levels of both genes were significantly decreased in the hepatopancreas, accompanied by a notable increase in the redness (a*) values. Furthermore, a significant increase in the β-carotene content was observed in the hepatopancreas when EsNinaBl-mRNA was suppressed, which suggests that EsNinaBl plays an important role in carotenoid cleavage, specifically β-carotene. In conclusion, our findings suggest that EsNinaBl and EsBCO1l may exhibit functional co-expression and play a crucial role in carotenoid cleavage in crabs.

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