Five different diets with different ratio of fish oil to vegetable oil were prepared. The biological index and proximate composition of Eriocheir sinensis fed with different diets were compared, and then sensory analysis, electronic nose (E‐nose) and headspace‐solid phase micro‐extraction combined with gas chromatography‐mass spectrometer (HS‐SPME‐GC‐MS) analysis were applied to determine the odor profile of E. sinensis. The results showed that partial replacement (50%–75%) of fish oil by vegetable oil (FO/VO) was beneficial to the weight increment, nutrition accumulation, and odor‐active compounds (OACs) formation of E. sinensis. A total of 7 and 11 OACs were detected in the hepatopancreas and gonad, respectively, these OACs contributed greatly to the overall odor profiles of E. sinensis when the dietary replacement levels were at 50% and 75%, respectively. The results could provide the guide for dietary fish oil replacement as well as improving the odor quality of E. sinensis. Practical application The objective of this research is to compare the effects of dietary replacement of fish oil by vegetable oil on proximate composition and odor profiles of E.sinensis. The results obtained from this study would not only chose an optimal dietary replacement level and serve as a useful database for the odor of female and crabs, but also provide some guide for the improvement of Chinese mitten crab aquaculture.

The hepatopancreas of Eriocheir sinensis are the key parts that form its unique flavor. Lipids are important parts of hepatopancreas; hence, this study used UHPLC‐Q E Orbitrap mass spectrometer to investigate the changes in the lipid composition of crabs formed from thermal oxidation system. The results demonstrated that key lipids in the hepatopancreas of female Chinese mitten crabs were phosphatidylethanolamine (PE) and free fatty acid (FFA) during the steaming process. The key fatty acids of PE were C18:1, C18:3, C20:3, C20:4, C20:5, and C22:6. The degradation rate of C24:0 in FFA was greater than the synthesis rate. Principal component analysis, partial least square analysis combined with hierarchical cluster analysis found that PE (16:0/20:5), PE (18:1/20:4), PE (16:0/22:6), PE (16:0/20:4), PE (16:0 /16:1), PE (16:0/18:2), PE (18:0/20:5), PE (18:0/22:6), PE (18:0/20:4), PE (16:0/18:1), PE (18:0/18:2), PE (18:0/22:5), and PE (18:0/18:1) were the key PE molecular species. Simulating thermal oxidation to understand the dynamic change mechanism of lipids is meaningful for processing of Chinese mitten crab products and catering to public sensory orientation. Practical applications In this study, the UHPLC‐Q E Orbitrap method was used to detect and analyze the molecular species changes of Eriocheir sinensis in the simulated thermal oxidation system, and systematically analyzed the law of changes. Based on these results, we can expand our understanding of the changing characteristics of the hepatopancreas and pancreas of the river crab and provide a direction for the formation mechanism of the aroma substances of E. sinensis during the heat treatment and the improvement of the quality of its products.