Key microbial communities steering the functioning of anaerobic digesters during hydraulic and organic overloading shocks

Regueiro, Leticia; Lema, Juan M.; Carballa, Marta

doi:10.1016/j.biortech.2015.08.076

Cited by 122 publications

(36 citation statements)

References 34 publications

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“…The increased biodiversity within the 519 codigester was therefore attributed to the growth of initially rare syntrophic bacteria, which were 520 also a driving force behind improvements in the codigester daily methane production and 521 increased values of q max,oleate . This finding agrees with previous observations that the abundance 522 of syntrophic bacteria was linked to anaerobic digester resilience (Regueiro et al, 2015;Werner 523 et al, 2011). 524…”

supporting

confidence: 93%

Microbial community adaptation influences long-chain fatty acid conversion during anaerobic codigestion of fats, oils, and grease with municipal sludge

et al. 2016

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Codigesting fats, oils, and greases with municipal wastewater sludge can greatly improve biomethane recovery at wastewater treatment facilities. Process loading rates of fats, oils, and greases have been previously tested with little knowledge of the digester microbial community structure, and high transient fat loadings have led to long chain fatty acid (LCFA) accumulation and digester upsets. This study utilized recently-developed quantitative PCR assays for syntrophic LCFA-degrading bacteria along with 16S amplicon sequencing to relate changes in microbial community structure to LCFA accumulation during transient loading increases to an anaerobic codigester receiving waste restaurant oil and municipal wastewater sludge. The 16S rRNA gene concentration of the syntrophic β-oxidizing genus Syntrophomonas increased to ∼15% of the Bacteria community in the codigester, but stayed below 3% in the control digester that was fed only wastewater sludge. Methanosaeta and Methanospirillum were the dominant methanogenic genera enriched in the codigester, and together comprised over 80% of the Archaea community by the end of the experimental period. Constrained ordination showed that changes in the codigester Bacteria and Archaea community structures were related to measures of digester performance. Notably, the effluent LCFA concentration in the codigester was positively correlated to the specific loading rate of waste oil normalized to the Syntrophomonas 16S rRNA concentration. Specific loading rates of 0-1.5 × 10(-12) g VS oil/16S gene copies-day resulted in LCFA concentrations below 30 mg/g TS, whereas LCFA accumulated up to 104 mg/g TS at higher transient loading rates. Based on the community-dependent loading limitations found, enhanced biomethane production from high loadings of fats, oils and greases can be achieved by promoting a higher biomass of slow-growing syntrophic consortia, such as with longer digester solids retention times. This work also demonstrates the potential for controlling the loading rate of fats, oils, and greases based on the analysis of the codigester community structure, such as with quantitative PCR measurements of syntrophic LCFA-degrading bacteria abundance.

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confidence: 93%

Microbial community adaptation influences long-chain fatty acid conversion during anaerobic codigestion of fats, oils, and grease with municipal sludge

et al. 2016

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“…). These results confirm previous observations on the importance of OLR and FAN levels on community organization of AD reactors under both mesophilic (Werner et al ., ; Regueiro et al ., , ) and thermophilic conditions (Yenigün and Demirel, ; Kuroda et al ., ). Interestingly, the largest community shift of this period occurred earlier in archaeal than in bacterial communities.…”

Section: Resultsmentioning

confidence: 99%

“…The organic loading rate (OLR) is another important factor shaping AD microbial communities. Organic overloading causes process imbalance, and thus volatile fatty acid (VFA) accumulation, and a shift in the microbial community Regueiro et al, 2015). Overloading events are also associated with an increase in free ammonia nitrogen (FAN) concentrations in the digesters when the substrate used is rich in nitrogen compounds such as manure (De Francisci et al, 2015;Regueiro et al, 2016;Sun et al, 2016) and some types of food waste (Gao et al, 2015;Yun et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Air‐side ammonia stripping coupled to anaerobic digestion indirectly impacts anaerobic microbiome

Fernandez‐Gonzalez

Pedizzi

Lema

et al. 2019

Microbial Biotechnology

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Summary Air‐side stripping without a prior solid–liquid phase separation step is a feasible and promising process to control ammonia concentration in thermophilic digesters. During the process, part of the anaerobic biomass is exposed to high temperature, high pH and aerobic conditions. However, there are no studies assessing the effects of those harsh conditions on the microbial communities of thermophilic digesters. To fill this knowledge gap, the microbiomes of two thermophilic digesters (55°C), fed with a mixture of pig manure and nitrogen‐rich co‐substrates, were investigated under different organic loading rates (OLR: 1.1–5.2 g COD l−1 day−1), ammonia concentrations (0.2–1.5 g free ammonia nitrogen l−1) and stripping frequencies (3–5 times per week). The bacterial communities were dominated by Firmicutes and Bacteroidetes phyla, while the predominant methanogens were Methanosarcina sp archaea. Increasing co‐substrate fraction, OLR and free ammonia nitrogen (FAN) favoured the presence of genera Ruminiclostridium, Clostridium and Tepidimicrobium and of hydrogenotrophic methanogens, mainly Methanoculleus archaea. The data indicated that the use of air‐side stripping did not adversely affect thermophilic microbial communities, but indirectly modulated them by controlling FAN concentrations in the digester. These results demonstrate the viability at microbial community level of air side‐stream stripping process as an adequate technology for the ammonia control during anaerobic co‐digestion of nitrogen‐rich substrates.

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“…It might be participating in the degradation of amino acids and some refractory organic matters [56][57][58]. The genus Smithella, which could degrade propionate to acetate [42,59], was dominant in start-up (3.13%) and winter (3.5%), and this was probably one of the reasons of acetate accumulation. The genus Acinetobacter was observed in autumn and winter, and was probably involved in the oxidation of organic matter or sulfides [13,60].…”

Section: Shifts In Microbial Community Structures With Seasonal Tempementioning

confidence: 99%

Effect of Seasonal Temperature on the Performance and on the Microbial Community of a Novel AWFR for Decentralized Domestic Wastewater Pretreatment

Lü

2017

Applied Sciences

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Due to environmental burden and human health risks in developing countries, the treatment of decentralized domestic wastewater has been a matter of great concern in recent years. A novel pilot-scale three-stage anaerobic wool-felt filter reactor (AWFR) was designed to treat real decentralized domestic wastewater at seasonal temperature variations of 8 to 35 • C for 364 days. The results showed that the average chemical oxygen demand (COD) removal efficiencies of AWFR in summer and winter were 76 ± 7.2% and 52 ± 5.9% at one day and three days Hydraulic Retention Time (HRT), respectively. COD mass balance analysis demonstrated that even though COD removal was lower in winter, approximately 43.5% of influent COD was still converted to methane. High-throughput MiSeq sequencing analyses indicated that Methanosaeta, Methanobacterium, and Methanolinea were the predominant methanogens, whereas the genus Bacillus probably played important roles in fermentation processes throughout the whole operation period. The performance and microbial community composition study suggested the application potential of the AWFR system for the pretreatment of decentralized domestic wastewater.

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Key microbial communities steering the functioning of anaerobic digesters during hydraulic and organic overloading shocks

Cited by 122 publications

References 34 publications

Microbial community adaptation influences long-chain fatty acid conversion during anaerobic codigestion of fats, oils, and grease with municipal sludge

Microbial community adaptation influences long-chain fatty acid conversion during anaerobic codigestion of fats, oils, and grease with municipal sludge

Air‐side ammonia stripping coupled to anaerobic digestion indirectly impacts anaerobic microbiome

Effect of Seasonal Temperature on the Performance and on the Microbial Community of a Novel AWFR for Decentralized Domestic Wastewater Pretreatment

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