Khảo sát thành phần hóa học và hoạt tính gây độc tế bào Hep-G2 của cây An Xoa (Helicteres hirsuta L.)

Duyên, Nguyễn Hữu; Phước, Lê Thanh

doi:10.22144/ctu.jvn.2016.605

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…To date, many studies on H. hirsuta have been carried out in Vietnam and around the world. Several significant pharmacological properties from different parts of the H. hirsuta have been demonstrated thus far, including antioxidant activity (1,(3)(4)(5)(6) ,analgesic and anti-inflammatory actions (7) ,toxic and antibacterial effects on several cancer cell lines in vitro (8)(9)(10)(11)(12) .Studying the effects of some extraction conditions and isolation of pharmaceutical ingredients from different parts of H. hirsuta has been of interest to many scientists (1,3,4,12) . Quang et al (2020) researched the isolation and acquired 12 compounds from H.hirsuta gathered in Binh Phuoc (13) , while Chin et al (2006) studied the isolation of active components in it originating in Indonesia and obtained six lignans (14) .…”

Section: Introductionmentioning

confidence: 99%

Bioactive Components of Helicteres hirsuta Collected in Thua Thien Hue

Long,

Le,

Quang

et al. 2023

IJST

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Objectives: In this study, we investigate the bioactive potential of Helicteres hirsuta Lour. (H. hirsuta L.). Methods: H. hirsuta collected in Thua Thien Hue province was used. The methanolic extract of roots and leaves was studied for its biochemical components based on the High-Performance Liquid Chromatography method and also tested for antioxidant activities Mention how the test was carried out and the endpoints considered. Findings : The results show that some biochemical components contained in the dry materials of H. hirsuta leaves and roots are safe and suitable, allowing use in the extraction and collection of total flavonoids. The optimal extraction conditions using the ultrasonic bath method included a time of 50 min, a material/solvent ratio of 1:20 (g/mL), ultrasonic frequency (12 Hz), and a solvent concentration of methanol of 50% (v/v). In this condition, the highest total flavonoid content obtained from H. hirsuta leaves and roots were 0.841 mg CE/mL extract (leaf) and 0.416 mg CE/mL extract (root), respectively. The DPPH free radical scavenging ability at 50% concentration (IC 50 ) of leaf and root extracts varied with IC 50 of 0.778 mL and 0.433 mL, respectively. The methanol extract from the leaf and root of H. hirsuta has the best inhibitory effect on the human liver cancer cell line Hep-G2 (IC 50 leaf = 177.22 µg/mL, and IC 50 root = 165.21 µg/mL). Novelty: The results of this study are valuable reference information for exploiting, using, and processing products from H. hirsuta trees collected in Thua Thien Hue in the future.

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Section: Introductionmentioning

confidence: 99%

Bioactive Components of Helicteres hirsuta Collected in Thua Thien Hue

Long,

Le,

Quang

et al. 2023

IJST

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Optimization of Total Flavonoid Extraction From the Helicteres hirsuta Lour. Roots by Bath Ultrasound Assisted method and cytotoxic activities of these Flavonoids

Hue

Long

et al. 2023

J Exp Bio & Ag Sci

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This study was carried out to optimize the various approaches to analyze the effects of various variables on the total flavonoid content extraction from the roots of Helicteres hirsuta L. The existence of various compounds in the methanol fraction was accessed by using LC-MS/MS analysis. The results of the study identified the ideal parameters such as times (30 minutes); methanol solvent concentration (50%); ultrasonic frequency (12 Hz); and material/solvent ratio [1:30 (w/v)] for extracting the highest total flavonoids from the roots of H. Hirsuta. The study's results suggested that the total flavonoid value was 3.52684 (mg Catechin/g extract). The verified experiment obtained an actual value of 5.205 (mg Catechin/g extract). Further, the results of the study suggested the presence of 20 compounds of a flavonoid nature (66.667%) appearing in the purified methanol fractional extract. These compounds can inhibit DPPH free radicals at 50%, with an IC50 value of 536.760 g/mL, and they also have inhibitory activity on the growth of cancer cell lines with IC50 values ranging from 115.81 and 219.17g/mL. The human leukemia cell line (HL-60) exhibits the most significant cytotoxic response to a methanol extract from H. hirsuta root with an IC50 value of 115.81 g/mL.

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Khảo sát thành phần hóa học và hoạt tính gây độc tế bào Hep-G2 của cây An Xoa (Helicteres hirsuta L.)

Cited by 2 publications

References 7 publications

Bioactive Components of Helicteres hirsuta Collected in Thua Thien Hue

Bioactive Components of Helicteres hirsuta Collected in Thua Thien Hue

Optimization of Total Flavonoid Extraction From the Helicteres hirsuta Lour. Roots by Bath Ultrasound Assisted method and cytotoxic activities of these Flavonoids

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