Ki-67 expression in mature B-cell neoplasms: a flow cytometry study

Abstract

SUMMARY OBJECTIVE: Ki-67 is a nuclear protein associated with cellular proliferation in normal or leukemic conditions that can help identify more aggressive diseases and is usually evaluated with immunohistochemistry. The aim of this was to assess Ki-67 expression on mature B-cell neoplasms samples with flow cytometry immunophenotyping. METHOD: After surface staining with CD19 and CD45, intracellular staining for Ki-67 was performed in leukemic mature B-cells. Ki-67 expression was evaluated with flow cytometry. RESULTS: Ki-67 expression was higher in mantle cell lymphoma, Burkitt lymphoma, and diffuse large B-cell lymphoma cases. It was also associated with CD38 mean fluorescence intensity. CONCLUSIONS: Ki-67 expression evaluated by flow cytometry can be a useful tool in the diagnosis of mature B-cell neoplasms. More studies are needed to validate Ki-67 assessment with flow cytometry immunophenotyping.

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“…The cells were finally stained with specific Ki-67 conjugated MoAb and incubated at 4 °C for 60 min in the dark. The fluorescent intensity of the labeled cells was analyzed using Becton Dickinson, FACS caliber flow cytometer operated with Cell Quest software (Becton Dickinson, Franklin Lakes, NJ) (Marcondes et al., 2018 ).…”